Loren Data's SAM Daily™

FBO DAILY ISSUE OF JULY 11, 2002 FBO #0221
MODIFICATION

B -- Perform Cloning and DNA Sequencing for USDA, Agricultural Research Station, Southern Regional Research Station, New Orleans, LA 70179

Notice Date

7/9/2002
 

Notice Type

Modification
 

Contracting Office

Department of Agriculture, Agricultural Research Service, Pacific West Area Office, 800 Buchanan Street, Albany, CA, 94710-1105
 

ZIP Code

94710-1105
 

Solicitation Number

RFP26-95CV-02
 

Response Due

8/26/2002
 

Archive Date

9/10/2002
 

Point of Contact

Louise Snitz, Contracting Officer, Phone 510-559-6022, Fax 510-559-6023,
 

E-Mail Address

lsnitz@pw.ars.usda.gov
 

Description

Provide complete sequence of aflatoxin biosynthetic gene cluster from 5 isolates of Apsedrgillus with an accuracy better than 1 error per 2000 pb (99.95%). The sequence is between 65 and 75 KB. ARS will provide high quality genomic DNA sequence information derived from a single aflatoxin producing strain. Sequence information is available for all genes in the cluster, as well as, most non-coding regions. Sequence is available for both ends and the middle of the cluster. The contractor will develop the necessary cloning strategy to separate the required sequence from other sequences, carry out the sequencing with sufficient coverage to provide the requisite accuracy, and perform sequence assembly. Finally, the contractor will provide the sequence in the format accessible to common databases, such as Genbank. Contractor will provide: 1) Cosmid, fosmid or BAC genomic libraries for each isolate. 2) Isolate clones from these libraries containing the entire 75 kb aflatoxin biosynthetic cluster using known sequence information for comparison. 3) Shotgun subclone form the socmid clones. 4) Sequence subclones to at least 6X coverage to provide sequence accuracy of >99.95%. 5) Assemble sequences from subclones. 6) Provide sequence in database accessible format. We would prefer a strategy based on cloning and shotgun sequencing. However, other strategies (i.e. long range PCR with high fidelity polymerase) will be considered.
 

Web Link

Link to FedBizOpps document.
(http://www.eps.gov/spg/USDA/ARS/PWAO/RFP26-95CV-02/listing.html)
 

Place of Performance

Address: USDA, REE, ARS, SRRC ATTN: Peter Cotty P.O. Box 19687 New Orleans, LA

Zip Code: 70179

Country: US
 

Record

SN00113026-F 20020711/020709214734 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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