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FBO DAILY ISSUE OF MARCH 19, 2003 FBO #0474
SOLICITATION NOTICE

66 -- 66?-Ratiometric Microscope Imaging System

Notice Date
3/17/2003
 
Notice Type
Solicitation Notice
 
Contracting Office
Department of Health and Human Services, National Institutes of Health, National Institute of Environmental Health Sciences, Acquisitons Management Branch, PO Box 12874, Research Triangle Park, NC, 27709
 
ZIP Code
27709
 
Solicitation Number
DLR30106
 
Archive Date
4/2/2004
 
Point of Contact
Deitra Lunney, Contracting Officer, Phone 919 541-0387, Fax 919 541-5117,
 
E-Mail Address
lunney@niehs.nih.gov
 
Description
The National Institute of Environmental Health Sciences intends to award a commercial sole source purchase order to Intracellular Imaging, Inc., Cincinnati, OH 45219, pursuant to 41 U.S.C. 253(c)(1)?Only one responsible source and no other supplies or services will satisfy agency requirements. This procurement is for the purchase of one (1) each InCyt Im2 Fluorescence Imaging System consisting of Imaging Workstation, Nikon TS-100F Inverted Microscope, Excitation Illumination Source, Computer, and Installation & Training. The imaging system must be based around a simple inverted microscope with dry objectives, both of which are optimized for UV excitation. The system (microscope, illumination system and computer) must have a small footprint due to limited space. Of major importance, because the system must be used by individuals with vastly differing levels of expertise, the software architecture should specifically direct the user in the appropriate method of performing a calcium experiment using the ratiometric calcium-dye technique. Hardware Requirements: 1) Inverted research microscope with infinity-corrected optics: 2) UV excitation wavelength(s) at 340 and 380 nm, i.e. suitable for excitation ratio imaging with Fura-2: 3) Additional filter set for viewing the following fluorochromes: YFP, GFP: 4) Microscope objectives: high NA 10x dry, high NA 20x dry: 5) Microscope stage that accommodates custom sample chambers in addition to standard 35mm petri dishes and 1"x3" slides: 6) Adjustable averaging or integration of detector/camera: 7) Continuous, and simultaneous adjustment of excitation intensity attenuation: 8) The ability to independently adjust the image intensity at each of the excitation wavelengths during data capture: 9) Host computer with flat panel display with at least 1280x1024 resolution and no less than 512 MB RAM. Software Requirements: 1) Software guides the user through the steps necessary for performing a correctly calibrated calcium imaging experiment: 2) Program configuration parameters can be saved and recalled under different configuration names: 3) Program/experiment parameters can be saved and recalled with an experiment's data: 4) Minimum acquisition time interval is no more than 1 sec for Fura-2 imaging of live cells: 5) Software saves images in TIFF format: 6) ROI (Region of Interest) kinetics analysis is available during acquisition as well as during post-acquisition analysis: 7) User can pause and resume acquisition: 8) User is limited to no less than 20 ROIs for concurrent measurement and graphing: 9) User has choice for ROI shape-- rectangular, elliptical, or user-defined: 10) ROIs can be different colors: 11) Image and graph are in separate windows, and can be displayed at the same time: 12) User chooses whether or not to measure ROIs: 13) User chooses whether or not to display graph(s): 14) Optional background subtraction is possible before calculating/plotting: 15) Ratios can be plotted for two-channel data: 16) ROI ratio data can be calibrated to ion concentrations, e.g. [Ca++] from fura-2 ratios, before plotting: 17) User has control over x and y ranges of graph: 18) Placement of text and markers on images and graphs is possible: 19) Images and graphs print out easily on user's demand: 20) Graph data can be output to an ASCII file. All responsible sources may respond by submitting a written narrative statement of capability within 15 days of date of notice to Dee Lunney at the above address for consideration by the agency. Response must include detailed technical information and other technical literature demonstrating the ability to meet these requirements. Responses shall also include itemized pricing that is sufficient to make a determination that competition is feasible. If no affirmative responses are received within 15 days of this notice to determine whether a qualified source is more advantageous to the Government, an order will be placed with Intracellular Imaging, Inc. The NAICS Code/Size is 334516/500EM. This procurement is to be processed using Simplified Acquisition Procedures. There is no solicitation package available. For any questions regarding this announcement, please contact Dee Lunney via email lunney@niehs.nih.gov.*****
 
Record
SN00280941-W 20030319/030317213416 (fbodaily.com)
 
Source
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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