SOLICITATION NOTICE
R -- Biologist
- Notice Date
- 3/19/2003
- Notice Type
- Solicitation Notice
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Nat'l Institute of Diabetes, Digestive, & Kidney Diseases, 2 Democracy Plaza, Suite 700W 6707 Democracy Blvd., MSC 5455, Bethesda, MD, 20892-5455
- ZIP Code
- 20892-5455
- Solicitation Number
- NIH-NIDDK-03-896
- Archive Date
- 4/23/2003
- Point of Contact
- Patricia Haun, Purchasing Agent, Phone (301) 594-8855, Fax (301) 480-4226,
- E-Mail Address
-
haunp@extra.niddk.nih.gov
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information contained in this notice. This procurement is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. This solicitation document and incorporated provisions and clauses are those in effect through the September 2001 Federal Acquisition Regulation revision, which includes the consolidation of all Federal Acquisition Circulars through 2001-12. It is the intent of the National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) to procure services of a Biologist from Henry M. Jackson Foundation, 1401 Rockville Pike, Suite 600, Rockville, MD 20852. The contractor should have extensive expertise in state-of-the-art Nuclear Magnetic Resonance (NMR) Spectroscopy, as well as complete familiarity with the relevant molecular biology and biochemical techniques to structural biology. The specific tasks are as follows and will be included in the Request for Quote (RFQ). The service will be for a Base Year with four (4) option years. The contractor shall provide personnel resources for research and development for the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) within the National Institutes of Health. The activities to be conducted under these categories concern the application of multidimensional nuclear magnetic resonance (NMR) spectroscopy to the determination of three-dimensional structures of proteins and protein compexes. A fundamental knowledge and understanding of state-of-the-art multidimensional NMR spectroscopy, of the application of this method to three-dimensional structure determination of larger proteins, of methods of protein expression, purification and mutagenesis, and of computational methods required to convert the experimental data into three-dimensional atomic structures, are key factors in accomplishing the tasks. Specifically the contractor must provide key personnel with a PhD degree that involved extensive use of macromolecular NMR spectroscopy and its application to 3D structure determination, in conjunction with molecular biology techniques. Task Area 1. Protein NMR Section, NIDDK Task 1. The Contractor shall provide support for the 3D structure determination of proteins using multidimensional NMR spectroscopy and associated computational techniques with special emphasis on complexes of the bacterial phosphotransferase (PTS) pathway. The task requires familiarity with NMR spectroscopy, biochemistry/molecular biology, and computer environments. The effort shall initially focus on the 60 kDA dimeric complexes of IIAmannose with HPr and IIBmannose , and will subsequently be extended to the 90 kDA trimeric complex of IIAlactose and HPr. The effort involves extensive interaction with personnel in the Protein NMR Section. Initial work will focus on expressing and purifying appropriate quantities of isotopically labeled (15N, 13C and/or 2H) proteins. This will be followed by the application of multidimensional separated and isotopically-filtered NMR experiments to derive (a) sequential backbone and sidechain assignments of the protein components within the complexes; (b) derivation of structural information in the form of interproton distances obtained from multidimensional nuclear Overhauser enhancement measurements, long range restraints from paramagnetic relaxation enhancement measurements, and orientational restraints from measurements in oriented samples in dilute liquid crystalline media; and (c) finally calculation of 3D structures from the experimental NMR data using computational algorithms involving the application of simulated annealing methodologies (and in particular torsion angle and conjoined rigid body/torsion angle dynamics). NIDDK has determined that based upon their experience and expertise, The Foundation is the only source with the unique qualifications that can successfully accomplish the specified tasks. This notice of intent is not a request for competitive proposals however, all responses received, within 20 days from the date of publication of this synopsis will be considered by NIH. A determination by the Government not to compete this proposed acquisition is based upon responses to this notice and is solely for the purpose of determining whether to conduct a competitive procurement. Request for copies of the Request for Quote (RFQ) must be in writing and may be faxed to (301) 480-8261. Quotations must be submitted in writing to the National Institute of Diabetes and Digestive and Kidney Disease, 6707 Democracy Blvd., Room 773, Bethesda, Maryland 20817. Attention: Diana Ly (301) 594-7730.
- Place of Performance
- Address: 9000 Rockville Pike, Bethesda, MD
- Zip Code: 20892
- Country: USA
- Zip Code: 20892
- Record
- SN00282773-W 20030321/030319222054 (fbodaily.com)
- Source
-
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |