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FBO DAILY ISSUE OF MARCH 28, 2003 FBO #0483
SOLICITATION NOTICE

66 -- Singer MSM System 300 Tetrad Dissection Microscope

Notice Date

3/26/2003
 

Notice Type

Solicitation Notice
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Research Contracts Br., 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
 

ZIP Code

20852
 

Solicitation Number

NCI-30037-NG
 

Archive Date

4/25/2003
 

Point of Contact

Malinda Holdcraft, Purchasing Agent, Phone (301) 402-4509, Fax (301) 402-4513, - Renita Smith, Contract Specialist, Phone 301-496-8612, Fax 301-480-0241,
 

E-Mail Address

holdcram@exchange.nih.gov, rs442i@nih.gov
 

Description

The National Cancer Institute (NCI), Laboratory of Molecular Cell Biology (LMCB), plans to procure a Singer MSM System 300 Tetrad Dissection Microscope from Singer Instrument Co. Ltd., Roadwater, Watchet, Somerset TA23 0RE England, United Kingdom. The acquisition is being conducted under the simplified acquisition procedures authorized in FAR Part 13. The North American Industry Classification System code is 334516 and the business size standard is 500 employees. The Laboratory of Molecular Cell Biology (LMCB) investigates basic cellular processes of homeostasis, growth, division and differentiation, with an emphasis on the genetic and molecular biology of chromosomes and the cell nucleus. Accordingly, LCMB is engaged in research on the structure of chromatin and how chromatin is packaged to regulate gene expression and maintain genomic integrity. The research involves the identification and characterization of interactions of chromosomal proteins and genes and the effects of these interactions on gene expression. Specifically, the project analyzes proteins involved in organization of the genome into higher-order structures and their effects on genome wide gene expression and maintenance of genomic integrity. This research involves extensive use of yeast genetic techniques, and requires a reliable tetrad dissection microscope system to manipulate the individual cells/spores produced from genetic crosses. The unique computerized control offered by Singer MSM 300 Tetrad Dissection System permits several functions that are not possible with other systems. For example, this system allows automation of repetitive aspects of the procedures described above. The microprocessor controller which, with its built in program for yeast manipulations, operates the motor driven stage automatically. The microprocessor always remembers the place that a cell or spore was deposited on the matrix allowing returning to same matrix points at later times for periodic observation of growing cells. Moreover, this system includes a high quality integral microscope and an easy to operate micromanipulator. The trinocular head of the microscope allows easy fitting of the CCTV and camera provided with this system, which is invaluable for techniques involving time-lapse image capture of cells. The X15 widefield eyepiece are coupled with a special XLWD X20 flatfield objectives; the ideal combination for yeast dissections and observation. Other unique features of this system include a LWD condenser with an anti-convection stage window and a hinged microscope overarm for easy loading of petri dishes containing yeast cultures. Finally, this is the only system that comes with a micro-zapper, an electronically controlled device that is necessary for efficient separation of spores during tetrad dissections. The Singer MSM System 300 Tetrad Dissection System is the only known piece of equipment on the market that meets the flexible requirements necessary for this type of research. Singer Instrument Company is the only source known to the NCI that can provide an instrument that can give the lab the capability and flexibility to conduct the described research. More specifically, the Singer MSM 300 computer-controlled tetrad dissection system shall allow NCI to automate many of the repetitive aspects of the necessary procedures such as the pedigree analysis of single cells, cell and zygote isolation, cell cycle progression of mutant cells and the genetic analysis of meiotic progeny produced during genetic crosses. This instrument shall allow the laboratory the capability to perform genetic analysis of highly conserved chromosomal proteins in the yeast Schizosaccharomyces pombe and to elucidate the function of these proteins in higher-order chromatin assembly and in regulating gene activity. This is not a request for competitive quotations. However, if any interested party believes it can provide the above supplies, it may submit a statement of capabilities. All information furnished shall be in writing and must contain sufficient detail to allow NCI to determine if it can meet the above minimum specifications described herein. Capability statements must be received in the Contracting Office by 1:00 PM EST (local Washington D.C. time) on April 10, 2003. If you have questions, please contact Malinda Holdcraft, Purchasing Agent via electronic mail at holdcram@excange.nih.gov or by fax at (301) 402-4513. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received shall be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted.
 

Place of Performance

Address: NIH/NCI, 9000 Rockville Pike, Bethesda, MD

Zip Code: 20892

Country: USA
 

Record

SN00288162-W 20030328/030326213311 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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