SOLICITATION NOTICE
66 -- Zeiss LSM 510 META Confocal Microscope system with FCS Confocor 2 Attachment
- Notice Date
- 8/8/2003
- Notice Type
- Solicitation Notice
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Research Contracts Br., 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
- ZIP Code
- 20852
- Solicitation Number
- Reference-Number-NCI-30107-NV
- Response Due
- 8/14/2003
- Archive Date
- 8/29/2003
- Point of Contact
- Deborah Moore, Purchasing Agent, Phone (301) 402-4509, Fax (301) 402-4513, - Caren Rasmussen, Lead Contract Specialist, Phone (301) 402-4509, Fax (301) 402-4513,
- E-Mail Address
-
dm170b@nih.gov, cr214i@nih.gov
- Description
- The National Institutes of Health (NIH), National Cancer Institute, Center for Cancer Research (CCR) Confocal Core Facility in the Laboratory of Receptor Biology and Gene Expression (LRBGE) plans to procure an LSM 510 META Confocal Microscope system with FCS Confocor 2 Attachment Configured with Motorized Axiovert 200 Inverted Research Microscope from Carl Zeiss Inc., One Zeiss Drive, Thornwood, NY 10594. The LRBGE has the need for a system that provides confocal analysis and fluorescence correlation spectroscopy of a wide range of biological specimens. This approach will enable sensitive analysis of molecular mobilities and interactions in living cells. This system can also function as a conventional confocal for fixed and live cell studies by using the visible Argon and HeNe lasers. The FCS capability will enable measurement of molecular mobilities as well as interactions between molecules. The Zeiss LSM 510 FCS has a fourth PMT, the Meta detector, which enables users to collect hard to separate fluorophore pairs like CFP/YFP and GFP/YFP. Since the Confocal Core Facility is a multi-user environment they require a system that is easy to operate and provides reliable quantitative data. This microscope provides the following features: 1) each detector is equipped with its own pinhole for the possibility of simultaneous scanning with identical slice thickness; 2) full 360 degree scan rotation, scan zoom and offset with no x/y stage centering; 3) digital scan control which allows for the following hardware/software functionality: constant scanning velocity for all speeds, even illumination along scanning fields with identical pixel conditions, short fly back time (less than 2% of frame cycle), on-line modification of scanning and detector settings, on-line histogram and lut's for optimization, sample preservation via beam blanking only in regions of interest and FRAP (Fluorescence Recovery After Photobleaching); 4) system is equipped with a spectral scanner that incorporates a 32 PMT using a grating for high speed, efficient, electronic switching between spectrally separated detector elements, both binning and spectral scanning is electronically managed by the software; 5) system is capable of emission fingerprinting with at least 10 nm collection upon delivery of any LSM and supports all acquisition modes x, y, z, t and lambda rapidly; 6) the spectral detector allows electronic high speed selection wavelength range including quick and easy switching between overlapping band pass channels; 7) optical correction of individual objective lenses via fully motorized collimation optics integrated and software controlled; and 8) system provides single button control to implement identical system conditions from any previously collected image. This system is commercial as defined in FAR Part 2, and this acquisition is being made in accordance with the test program using simplified procedures for certain commercial items as authorized in FAR 13.5. Carl Zeiss Inc. is the only organization known to the NCI researcher that has all the aforementioned features that are required and believed to be unique. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement and the noted features, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can perform this requirement. An original and one copy of the capability statements must be received in the contracting office by 1:00 p.m. EDT, on August 14, 2003. All questions must be in writing and can be faxed to (301) 402-4513 or emailed to dm170b@nih.gov . It is the vendor?s responsibility to call (301) 402-4509 to insure questions have been received. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect call will be accepted. NAICS 334516
- Record
- SN00395985-W 20030810/030808213411 (fbodaily.com)
- Source
-
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