Loren Data's SAM Daily™

FBO DAILY ISSUE OF SEPTEMBER 05, 2003 FBO #0647
MODIFICATION

B -- Design and Synthesis of 70-mer Oligonucleotide Probes

Notice Date

9/3/2003
 

Notice Type

Modification
 

Contracting Office

Department of Agriculture, Agricultural Research Service, Pacific West Area Office, 800 Buchanan Street, Albany, CA, 94710-1105
 

ZIP Code

94710-1105
 

Solicitation Number

003-91H2-03
 

Response Due

9/19/2003
 

Archive Date

9/19/2003
 

Point of Contact

Clarice Dixson, Contract Specialist, Phone 510-559-6027, Fax 510-559-5638,
 

E-Mail Address

cdixson@pw.usda.gov
 

Description

The USDA, Agricultural Research Service in Albany, California has a requirement for the design and synthesis of 70-mer Oligonucleotide probes for microarray experiments. Must provide full bioinformatics service to design 10,000-20,000 70-mer oligonucleotides from private or public assembly of wheat expressed sequence tags (EST). Designed from 750-1000 bp from the 3? end of available gene; Tm normalized and designed to minimize homopolymeric stretches, highly repetitive regions, low-complexity regions, and secondary structure. The sequence must be optimized by BLAST against all known open reading frames (ORF) to minimize cross-hybridization. The synthesis must be 70-mer oligonucleotide probes; quality control by OD and batch PAGE or CE analysis; and provided as salt-free, concentration normalized and lyophilized in 96-well plate format. Must provide free aliquoting of probes into 3-4 daughter plates; 200 nM synthesis scale with 16-18 nmole guaranteed yield; and provide a 96-well test plate of oligos for printing and hybridization test. Delivery must be two weeks from receipt of oligo order.
 

Place of Performance

Address: USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, California

Zip Code: 94710

Country: USA
 

Record

SN00423401-W 20030905/030903213156 (fbodaily.com)
 

Source

FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)

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