SOLICITATION NOTICE
R -- Biologist
- Notice Date
- 11/19/2003
- Notice Type
- Solicitation Notice
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Nat'l Institute of Diabetes, Digestive, & Kidney Diseases, 2 Democracy Plaza, Suite 700W 6707 Democracy Blvd., MSC 5455, Bethesda, MD, 20892-5455
- ZIP Code
- 20892-5455
- Solicitation Number
- NIH-NIDDK-04-152
- Response Due
- 12/4/2003
- Archive Date
- 12/19/2003
- Point of Contact
- Patricia Haun, Purchasing Agent, Phone (301) 594-8855, Fax (301) 480-4226,
- E-Mail Address
-
haunp@extra.niddk.nih.gov
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NIH-NIDDK-04-152, and is issued as a Request for Quotation (RFQ). This procurement is being conduced under Simplified Acquisition Procedures in accordance with FAR Part 13. This solicitation will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2001-14. This acquisition is 100% small business set-aside and the NAICS code is 621511 with a small business size standard $11.5 million. It is the intent of the National Institutes of Health (NIH) to purchase the services of a Biologist to Maintain and Generate Muscarinic Receptor Mutant Mice and Assist in their Molecular and Physiological Analysis in the Laboratory of Biooranic Chemistry from Yinghong Cui, 11171 Captains Walk CT., Gaithersburg, MD 20878. The service will be for a Base Year with four (4) option years. Duties to be performed by the Biologist shall be: A) Animal Husbandry: ? Maintains multiple mutant mouse colonies housed in Bldg. 14C-North (4 racks) and Bldg. 8 (1 rack) (about 2000 mice total) ? Identifies all mice by ear tags ? Sets up matings to ensure that the different mutant mouse lines are present at sufficient numbers and ages ? Keeps electronic records of all of Dr. Wess? mutant mice housed in Bldg. 14C-North and Bldg. 8. For each individual mouse, these records will show mouse sex (male or female), date of birth, genetic background, generation number, and whether or not the mouse has a specific mutation ? Generates mice by homozygous or heterozygous matings for experiments to be carried out in Dr. Wess? lab or by Dr. Wess? collaborators. It is estimated that 1500 mice will be needed per year ? Uses PCR or Southern blot analysis to determine whether the mice used for experiments are wild-type or mutant mice. Based on this analysis, mice will be classed into different experimental groups. B) Lab Work (to be carried out in Bldg. 8A): ? Assists in the generation of conditional muscarinice receptor mutant mice by carrying out PCR or Southern blot analyses to identify mouse embryonic stem cells and, subsequently, mice that carry the desired mutation ? Assists in the molecular and biochemical analysis of muscarinic receptor mutant mice by studying the expression patterns of individual muscarinic receptors. This will require the use of RT-PCR, radioligand binding, Western blotting and immunoprecipitation techniques ? Assists in the physiological analysis of muscarinic receptor mutant mice. Specifically, islets will be prepared from the mouse pancreas of wild type and muscarinic receptor mutant mice. The ability of the pancreatic islets to release insulin following different physiological stimuli (e.g. glucose, acetylcholine) will be examined. This analysis will show which muscarinic receptor subtypes are involved in the regulation of insulin release. The individual shall have a Bachelor?s degree in Biology or a related science. It is also necessary that the individual work 40 hours a week. NIDDK has determined that Yinghong Cui is qualified to accomplish the specific tasks, listed above, and based upon her previous experience and expertise, in the Section of Molecular Signaling, LBC. This notice of intent is not a request for competitive quotations however, all responses received, within 15 days from the date of publication of this synopsis will be considered by the Government. A determination by the Government not to compete this proposed acquisition is based upon responses to this notice and is solely for the purpose of determining whether to conduct a competitive acquisition. The offeror must include a completed copy of the provision of FAR Clause 52.212-3, Offeror Representations and Certifications ? Commercial Items with its offer. The provisions of FAR Clause 52.212-4, Contract Terms and Conditions ? Commercial Items, applies to this acquisition. The FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders ? Commercial Items ? Deviation for Simplified Acquisitions, applies to this acquisition. The clauses are available in full text at http://www.arnet.gov/far. Interested vendors capable of furnishing the government with the item specified in this synopsis should submit their quotation to the below address. Quotations will be due fifteen (15) calendar days from the publication date of this synopsis or December 4, 2003. The quotation must reference ?Solicitation Number? NIH-NIDDK-04-152. All 8(a) small businesses may submit a quotation, which if timely received, shall be considered by the agency. Quotations must be submitted in writing to the National Institutes of Diabetes and Digestive and Kidney Diseases, 6707 Democracy Blvd., Room 774C, Bethesda, Maryland 20817, Attention: Patricia Haun. Faxed copies will not be accepted.
- Place of Performance
- Address: 9000 Rockville Pike, Bethesda, md
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN00472562-W 20031121/031119211904 (fbodaily.com)
- Source
-
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)
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