SPECIAL NOTICE
66 -- Genetic Analyzer
- Notice Date
- 4/20/2005
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, Center for Disease Control and Prevention, Procurement and Grants Office (Atlanta), 2920 Brandywine Road, Room 3000, Atlanta, GA, 30341-4146
- ZIP Code
- 30341-4146
- Solicitation Number
- Reference-Number-000HCRJ1-2005-20120
- Response Due
- 4/25/2005
- Archive Date
- 4/25/2005
- Description
- The Centers for Disease Control and Prevention intends to issue a sole source purchase order to Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA., for a 3130 Genetic Analyzer. The instrument required is a 4-capillary, fluorescence-based genetic analysis system. It must be fully automated from polymer loading and replacement, DNA separation, detection, and data analysis. Run conditions must be optimized for several applications, such as de novo or comparative DNA sequencing and DNA fragment analysis. A full complement of chemistry kits, software, and accessory products should be available from the vendor. The instrument must be capable of analyzing multiple runs of samples?4 samples per run. The system must be fully automated from polymer loading to analyzed sequence or size-called results. The instrument must use a computer workstation for instrument operation and data analysis?specifically a Dell Workstation running a Windows XP or higher operating system. The Windows XP operating system should also be designed for networking with a larger laboratory management-computing environment. The system should use software for sample import and instrument control that can regulate the functions of the instrument and automatically process the data once the instrument has detected it. It must also provide several options for instrument diagnostics and automatic import of sample plate information. The instrument must use analysis software and algorithms that perform either basecalling for DNA sequencing or size calling for DNA fragment analysis. One application software type must be included with the purchase of the instrument system, the other must be available to be selected as an add-on kit. Data files should be generated in industry standard ABIF format, which must be viewed on a Windows computing system. The instrument must operate without user intervention (unattended) for 24-hours with a sample processing throughput greater than 144 sequencing or 144 fragment analysis samples per 24-hours. The system must be able to detect and analyze five fluorescent dyes simultaneously. The instrument must have: four capillaries, automated polymer delivery system, autoloading of samples performed from a single 96-well microtiter plate, CCD detection technology and a spectrograph for color separation, simultaneous dual-side illumination detection system to maximize signal uniformity and sensitivity that in turn reduces the requirements placed on the user for sample preparation and cleanup, active temperature cooling/heating that can maintain temperatures from 15 to 65 degrees C., a thermally stable detection region of the capillary array. Reagents optimized for use with this instrument must be available through the instrument?s vendor. The vendor must supply application-specific kits that are optimized for the instrument in the area of human identification, agriculture, molecular microbiology, and genetic disease research . The instrument must include software that has: electronic sample information that can be automatically imported from an external data-handling system, chemometric algorithmic processing of raw signal data, basecalling and size-calling algorithms that have been optimized for data from the instrument, instrument verification to check status of critical system components, available options for further downstream data analysis and data management, ability to run sequencing and fragment samples on a single plate, ability for the user to customize the run order, security, audit trail and electronic signature features that assist with 21 CFR Part 11 requirements.. The system?s capillary arrays and polymers must have the following specifications: support for POP-4?, POP-6?, and POP-7? Polymers, dynamically coat the capillary walls to control for electro-osmotic flow, have capillary arrays consisting of 4 capillaries, have capillary arrays available in four different sizes: 22 cm, 36 cm, 50 cm, and 80 cm, employ capillary arrays that use bare silica capillaries with a useful life that exceeds 150 runs. The instrument must be able to perform with: unattended operation, one to several runs spanning a period of 24 hours; rapid sequencing: using DNA control standards and protocol provided with an installation kit, the genetic analyzer must sequence over 500 bases at 98.5% accuracy with no more than 2% N-called bases, it should handle 41 runs with up to 164 samples in 24 hours; standard sequencing: using DNA control standards and a protocol provided with an installation kit, the genetic analyzer must sequence over 850 bases at 98.5% accuracy with no more than 2% N-called bases, it should handle 12 runs with up to 48 samples in 24 hours; long read sequencing: using DNA control standards and protocol provided with an installation kit, the genetic analyzer must sequence over 950 bases at 98.5% accuracy with no more than 2% N-called bases, it should handle 8 runs with up to 32 samples in 24 hours; DNA sizing: using DNA control standards and a protocol provided with an installation kit, the genetic analyzer must provide single-base detection of up to 400 bases with 0.15 standard deviation, it should handle 41 runs with up to 164 fragment analysis samples in 24 hours; Mutation validation/screening: single nucleotide polymorphism identification using five-color fluorescence with up to 120 bases. If using a 10-loci multiplex kit, it should handle 48 runs with up to 1,920 genotypes in 24 hours on a 36 cm array, single nucleotide polymorphism identification using five-color fluorescence with up to 120 bases. If using a 10-loci multiplex kit, it should handle 96 runs with up to 3,840 genotypes in 24 hours on a 22 cm array. The purchase of the instrument must include: system installation and operator training performed by a vendor service engineer, a one-year warranty on hardware parts and labor, a 90-day warranty of software and chemistry kits, service contracts offered for additional years of service. Purchase of the system must include: local service engineers, regional technical support/applications training, on-site, in-lab customer training, technical phone support, support via the Internet, support via a fax-back document system. CDC believes that this requirement is met by only one provider. This procurement will be processed under the authority of FAR 6.302-1 and 6.302-2. Only one responsible source and no other sources will satisfy agency requirements. No solicitation is being issued. Interested persons may identify their interest and capability to respond to this requirement. However, information received will be considered solely for information purposes only. This procurement is not set-aside for small business. For contractual questions contact Beth West at llw5@cdc.gov.
- Place of Performance
- Address: Atlanta, GA
- Zip Code: 30341
- Country: United States
- Zip Code: 30341
- Record
- SN00792538-W 20050422/050421083435 (fbodaily.com)
- Source
-
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