SOLICITATION NOTICE
66 -- Realtime PCR Thermocycler (SmartCycler)
- Notice Date
- 6/15/2005
- Notice Type
- Solicitation Notice
- Contracting Office
- Contracts Management Division 26 W. Martin Luther King Drive Cincinnati, OH 45268
- ZIP Code
- 45268
- Solicitation Number
- PR-CI-05-10948
- Response Due
- 7/27/2005
- Archive Date
- 8/28/2005
- Small Business Set-Aside
- N/A
- Description
- In accordance with FAR Part 12 and Part 13 Commercial and Simplified procedures, the U.S. Environmental Protection Agency intends to seek a quotation on a sole source basis with Cepheid of Sunnyvale, California to provide two (2) SmartCycler Systems. The Water Infrastructure Protection Division (WIPD) has on-going research and assay development that requires quantitative PCR using real-time thermocycler technology. EPA market research indicates the Cepheid SmartCycler is unique among all real-time PCR thermocyclers in that the Cepheid Smartcycler has individualized programmable sample sites that run different protocols simultaneously. Only the Cepheid SmartCycler is capable of running 4 dyes in specified excitation channels simultaneously in each sample processing site using individual protocols for each sample site. No other thermocycler met all of the criteria outlined below. To meet the needs of the WIPD methods development two thermocyclers with the following specifications are required. 1. The instrument must be designed to perform both traditional PCR and realtime PCR. 2. The instrument must have individually programmable sample processing sites that can simultaneously run individual PCR protocols. Each sample processing site must have independent thermal cycling and optical control systems. 3. The instrument must be able to detect four spectral bands using high-intensity LED emitters and silicon detectors for 4 color real-time, simultaneous, optical excitation and detection in each sample processing sites. Detection should not be accomplished via CCD camera. 4. The instrument must be capable of simultaneous excitation in the following ranges: (450-495 nm, 500-550 nm, 565-590 nm, 630-650 nm). 5. The instrument must be factory calibrated to optimally simultaneously detect 4 of the following fluorescent dyes: FAM, Cy3, TET, Texas Red, or Cy5 for Taqman, Beacon, Scorpion, Amplifluor fluorescent probes. 6. The instrument must be factory calibrated to produce melt-curve analyses with SYBR green chemistries. 7. The instrument must have at least 16 sample reaction sites and be expandable to 96 sample reaction sites, but not be in the 96-well microplate format. 8. The instrument must not utilize glass capillary-based sample reaction tubes. 9. The instrument must be capable of performing to factory calibrated standards in a field lab environment. The instrument must be of sufficient size to be transported in a hard carrying case for field environments. THIS NOTICE OF INTENT IS NOT A REQUEST FOR COMPETITIVE PROPOSALS. However, interested parties may identify their interest and capability and respond to the office identified above. Documentation must be received by June 27, 2005 in order to be considered by the EPA. A determination by the Government not to compete this proposed contract based upon assessment of responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Interested parties should submit a written response to Matthew J. Growney by fax (513) 487-2107 or by e-mail growney.matthew@epa.gov to receive consideration for this contract. Telephone responses will not be honored.
- Web Link
-
The Environmental Protection Agency
(http://www.epa.gov/oam/solicit)
- Record
- SN00830580-W 20050617/050615212505 (fbodaily.com)
- Source
-
FedBizOpps.gov Link to This Notice
(may not be valid after Archive Date)
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