SOLICITATION NOTICE
R -- CONDUCT MICROARRAY ANALYSES AND ASSAYS FOR OXIDATIVE INJURY
- Notice Date
- 7/15/2005
- Notice Type
- Solicitation Notice
- NAICS
- 541990
— All Other Professional, Scientific, and Technical Services
- Contracting Office
- Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), Western Administrative Support Center, 7600 Sand Point Way, Northeast, Seattle, WA, 98115-6349
- ZIP Code
- 98115-6349
- Solicitation Number
- WASC-5-1041
- Response Due
- 7/29/2005
- Archive Date
- 8/13/2005
- Description
- ASSIST WITH THE DEVELOPMENT OF NEW EXPERIMENTAL METHODS TO INVESTIGATE THE SUBLETHAL IMPACTS OF CURRENT USE PESTICIDES ON NEUROBIOLOGY AND BEHAVIOR OF THREATENED OR ENDANGERED SPECIES IN THE PACIFIC NORTHWEST. EFFORT IS BEING CONDUCTED FOR THE NATIONAL MARINE FISHERIES SERVICE/NW FISHERIES SCIENCE CENTER (NMFS). THE STUDY WILL FOCUS ON THE OLFACTORY NERVOUS SYTEM, WHICH UNDERLIES BEHAVIORS THAT CONTRIBUTE TO THE SURVIVAL, DISTRIBUTION, AND REPRODUCTION OF WILD PACIFIC SALMON. PERIOD OF PERFORMANCE IS 8/1/2005 ? 12/31/2006. WORK WILL INVOLVE THE FOLLOWING SPECIFIC TASKS: 1) CONDUCT MICROARRAY ANALYSES TO MONITOR CHANGES IN TRANSCIRPTONE OF SALMON OLFACTORY RECEPTOR NEURONS IN RESPONSE TO SUBLETHAL PESTICIDE EXPOSURES; 2) CONDUCT ASSAYS FOR OXIDATIVE INJURY IN PESTICIDE EXPOSED SENSORY NEURONS; 3) ANALYZE SALMON SENSORY NEURONS FOR INDICATIONS OF PESTICIDE-INDUCED APOPTOSIS; AND 4) DEVELOP EIGHT NEW RQ-PCR ASSAYS USING SYBERGREEN TO VALIDATE THE RESULTS OF THE MICROARRAYS. NMFS HAS IDENTIFIED THE UNIVERSITY OF WASHINGTON AS THE ONLY RESPONSIBLE SOURCE THAT CAN PERFORM THE REQUIRED STUDY. DR. GRAHAM YOUNG POSSESSES OVER 25 YEARS WORKING IN AREAS OF FISH PHYSIOLOGY AND ENDOCRINOLOGY, WITH A FOCUS ON REPRODUCTION IN FISH. DR. YOUNG?S LABORATORY HAS DEVELOPED UNIQUE METHODS AND REAGENTS REQUIRED FOR THIS RESEARCH. HIS LAB HAS DEVELOPED SEVERAL IN VITRO MODEL SYSTEMS TO STUDY EFFECTS OF ENDOGENOUS AND XENOBIOTIC COMPOUNDS ON STEROID PRODUCTION. THE MOLECULAR CLONING WORK HAS LED TO THE DEVELOPMENT OF A NUMBER OF QUANTITATIVE REAL-TIME PCR ASSAYS FOR THE MEASUREMENT OF GENE TRANSCRIPTS WITHIN THE GONAD, WHICH ARE REQUIRED TO DEVELOP SENSITIVE BIOMARKERS FOR CONTAMINANT EXPOSURE STUDIES. THESE ASSAYS ARE NOT COMMERCIALLY AVAILABLE. FURTHER, DR. YOUNG IS A WIDELY-PUBLISHED EXPERT IN AREA OF STEROID BIOSYNTHESIS IN FISH. SOURCES WITH SIMILAR QUALIFICATIONS AND EXPERIENCE MAY SUBMIT SAME IN WRITING. SUBMISSIONS WILL ONLY BE USED TO DETERMINE WHETHER TO SEND OUT A COMPETITIVE SOLICITATION. THIS IS A SIMPLIFIED ACQUISITION WITH AN ESTIMATED VALUE OF LESS THAN $100,000.
- Record
- SN00848127-W 20050717/050715211846 (fbodaily.com)
- Source
-
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