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FBO DAILY ISSUE OF MAY 18, 2006 FBO #1634
SPECIAL NOTICE

66 -- Equipment for Multiplex Detection of Botulinum Toxin Genes A Through G

Notice Date

5/16/2006
 

Notice Type

Special Notice
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

Department of Health and Human Services, Center for Disease Control and Prevention, Procurement and Grants Office (Atlanta), 2920 Brandywine Road, Room 3000, Atlanta, GA, 30341-4146
 

ZIP Code

30341-4146
 

Solicitation Number

000HCRP9-2006-31561
 

Response Due

5/23/2006
 

Archive Date

5/24/2006
 

Description

The Centers for Disease Control and Prevention intends to issue a sole source purchase order to Applied Biosystems, 850 Lincoln Center Drive, Foster City, CA 94404-1128 for purchase of a system for the purpose of multiplex detection of botulinum toxin genes A through G. The requirements for a system to accomplish this task are as follows: the instrument must be an integrated system designed to perform both real-time PCR (polymerase chain reaction) and post-PCR (end-point) analysis. The instrument must be capable of analyzing 96 samples simultaneously in a 96-well plate format. The instrument must provide specialized application specific software that collects and analyzes the fluorescence data for the applications of absolute quantitation, relative quantitation, allelic discrimination/SNP (Single Nucleotide Polymorphism) detection and plus/minus assays that utilize internal positive controls. The relative quantitation software should incorporate the ability to simultaneously visualize and analyze up to ten 96-well plates of gene expression data. The instrument must support two homogeneous reaction chemistries, the fluorogenic 5' nuclease assay using TaqMan? probes and the SYBR? Green I double stranded DNA binding dye chemistry. The instrument must utilize a tungsten-halogen lamp, a cooled charge coupled device (CCD) camera, and an optical system which utilizes multiple filters sets to enable multiple wavelength detection. Instrument software should utilize a multicomponenting algorithm to provide precise deconvolution of multiple dye signals, to enable the simultaneous detection of multiple fluorophores with reduced crosstalk. The five position filter wheel must have individual filters designed for use with a dedicated set of fluorescent dyes, which are available from the vendor as reporter dyes on fluorogenic probes. The instrument must be able to perform quantitative real-time PCR applications in less than 40 minutes in a 96-well format consumable with out the use of glass capillary tubes. The instrument must be capable of supporting three thermal cycling modes; Fast, standard and 9600 emulation. A heated lid assembly must heat the top half of the sample plates and their sealing devices so that no refluxing occurs in the sample plates. The heated lid assembly must apply sufficient sealing force to the reaction plate to ensure effective sealing and minimize reaction mixture evaporation. The instrument must have real-time quantitative PCR installation specifications which demonstrate the ability to distinguish between 5,000 and 10,000 template copies with a 99.7% confidence level. The instrument must be supplied with automated oligonucleotide design software. The instrument must have a footprint of less than 237 in2 (1,530 cms2) and must be supplied with an optional notebook computer to further reduce complete system footprint. The instrument must be supplied with either a dedicated notebook or tower computer platform (Pentium? M Centrino or Pentium? IV or above) running the Windows? XP Operating System. A factory-trained service engineer must be available to perform installation and all repairs. Optional service contracts after warranty period must be offered. The vendor must be able to supply all the necessary consumables to perform real-time quantitative PCR, and SNP genotyping, including PCR reagents designed for use with the fluorogenic 5' nuclease assay, PCR reagents designed for use with SYBR? Green I dye assay chemistry, fluorogenic probes, reaction plates and adhesive plate sealing covers. All PCR reagents should contain a passive internal reference dye to minimize well-to-well variability. The vendor must provide comprehensive assay design and development guidelines for real-time quantitative PCR and SNP genotyping assays. The vendor must be able to offer Telephone Technical Support and Field Applications/Sales/Service Support to help solve chemistry and instrumentation problems encountered with real-time quantitative PCR and SNP genotyping. CDC believes that this requirement is met by only one provider. This procurement will be processed under the authority of FAR 6.302-1 and 6.302-2. Only one responsible source and no other sources will satisfy agency requirements. No solicitation is being issued. Interested persons may identify their interest and capability to respond to this requirement. This procurement is not set-aside for small business. For contractual questions contact Linda M. Young.
 

Place of Performance

Address: 1600 Clifton Road, Building 17, Atlanta, GA

Zip Code: 30341

Country: USA
 

Record

SN01048781-W 20060518/060516220325 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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