SOLICITATION NOTICE
66 -- Amaxa Nucleofector 96-Well Shuttle
- Notice Date
- 6/2/2006
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
- ZIP Code
- 20852
- Solicitation Number
- Reference-Number-NCI-60076-NV
- Response Due
- 6/13/2006
- Archive Date
- 6/28/2006
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Genetics Branch (GB) plans to enter into a sole source procurement for a Amaxa Nucleofector 96-Well Shuttle and Nucleofector II Device from Amaxa Inc., 205 Perry Parkway, Suite 7, Gaithersburg, MD 20877. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. This acquisition will be processed under FAR Part 12 ? Acquisition for Commercial Items. Amaxa Inc. is the only source known to the NCI researcher that has a 96 well shuttle system that provides features that are required for their research and detailed below in their JOFOC. However, if any interested party believes it can perform requirement as detailed in the JOFOC they may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can perform this requirement. An original and one copy of the capability statement must be received in the contracting office by 1:00 pm EDT, on June 13, 2006. Faxed and emailed capability statements are NOT authorized. All questions must be in writing and may be emailed to Debbie Moore at dm170b@nih.gov or faxed (301) 402-4513. It is the vendor?s responsibility to call (301) 402-4509 and verify that questions were received. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award from the NCI contractors must be registered in the Online Representations and Certifications Applications (ORCA). Please refer to http://orca.bpn.gov in order to register. In addition, contractors must be registered in the Central Contractor Registration (CCR) www.ccr.gov. No collect calls will be accepted. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION Part I: Background information and Description of Acquisition Title: Acquisition of Amaxa Nucleofactor II/ HT 96 Shuttle System. Purpose/Brief History: Electroporation is a commonly used methodology for transferring nucleic acids, including RNA into cells. The Amaxa system (a curvette based, low throughput system) is widely used by investigators at NCI particularly for transfection of cell types such as primary cells that cannot be transfected by other methods. The mission of the Gene Silencing Section is to use the transfer of specific RNA molecules into cells to study gene function with a particular emphasis on performing this type of analysis on a high throughput basis. The new Amaxa 96 well based shuttle and Nucleofector II system is the first electroporation system that has been developed that will enable us to perform high throughput based electroporation of RNA into cell types that many of our NCI collaborators have requested us to consider for large scale gene function analysis. Amaxa Inc. has developed a high throughput 96 well plate module that is the only one equipped to work with the current Amaxa Nucleofector system (serial number: 240-582), based on their proprietary discovery of how to insert transfected DNA and RNA into the nucleus of cells. The Amaxa Inc. Nucleofector II and 96 Well HT Shuttle module work together to transfects 96 well plates of cDNA, shRNA and/or siRNA into primary cells and cell lines with transfection efficiency up to 90%. This is accomplished by transfecting the selected target directly into the nucleus of the cells, followed by selection of specific electrical parameters which have been customized for individual cell types. Current commercial methods cannot provide this type of transfection efficiency, viability or flexibility. This company also has three separate patents pending which make their technology unique and not readily available from other commercial sources. The Amaxa Nucleofector II and the HT 96 well Shuttle Module can transfect both primary cell types and individual cell lines. In addition to cDNA, shRNA or siRNA libraries (targets) transfection capability, the Amaxa 96 Well HT Shuttle module has the capabilities to transfect from 1 individual cell type up to 96 different cell types at a time. The Shuttle also has optimized protocols to transfect selected targets at high efficiency and high cell viability into primary cells and cell lines. These characteristics are unique to the Nucleofector II / Shuttle System. The results can only be obtained by purchasing the HT 96 well Shuttle module that works with the current Amaxa Nucleofector II instrument. No other manufacturer has access to this proprietary Technology which includes the reagents and instrument. PART II: Facts and reasons to Justify Other than Full and Open Competition Statutory authority FAR 13.106(b)(1) b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). Justification: As stated above Amaxa Inc is the only manufacturer of such an instrument (Nucleofactor II/HT 96 Shuttle System) that can insure 90% efficiency of transfecting cDNA, shRNA and /or siRNA into primary cells and cell lines in 96 well plates.
- Record
- SN01061306-W 20060604/060602220431 (fbodaily.com)
- Source
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