SOLICITATION NOTICE
66 -- Stopped Flow Instrument for the Analysis of Rapid Enzymatic Reactions
- Notice Date
- 8/18/2006
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd. EPS Suite 600, Rockville, MD, 20852
- ZIP Code
- 20852
- Solicitation Number
- Reference-Number-NCI-60153-NV
- Response Due
- 8/28/2006
- Archive Date
- 9/12/2006
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Cellular Oncology (LCO) plans to enter into a sole source procurement for a KinTek SF-2400 Stopped Flow Instrument from KinTek Corportion, 7604 Sandia Loop, Suite C, Austin, TX 78735. The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. This acquisition will be processed under FAR Part 12 ? Acquisition for Commercial Items. KinTek Corporation is the only source known to the NCI researcher that has a stopped-flow instrument that provides features that are required for their research and detailed below. However, if any interested party believes it can perform the requirement as detailed they may submit a statement of capabilities. The statement of capabilities and any other furnished information must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can perform this requirement. An original and one copy of the capability statement must be received in the contracting office by 1:00 pm EDT, on August __, 2006. Faxed and emailed capability statements are NOT authorized. All questions must be in writing and may be emailed to Debbie Moore at dm170b@nih.gov or faxed (301) 402-4513. It is the vendor?s responsibility to call (301) 402-4509 and verify that questions were received. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award from the NCI contractors must be registered in the Online Representations and Certifications Applications (ORCA). Please refer to http://orca.bpn.gov in order to register. In addition, contractors must be registered in the Central Contractor Registration (CCR) www.ccr.gov. No collect calls will be accepted. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION Part I: Background information and Description of Acquisition Title: Stopped flow analysis of rapid reactions. Purpose/Brief History: The Section on Membrane Trafficking studies a family of proteins that catalyze the hydrolysis of GTP bound to the small G-protein Arf. The current work is motivated by two critical findings. First, two members of the family, ASAP1 and ASAP3, contribute to malignancy. ASAP1 is a target of Src that is necessary for the formation of invadopodia in mammary carcinoma cells and has been identified as an oncogene in uveal melanoma. ASAP3 has been found to contribute to the pathology of hepatocellular carcinoma. Second, although at its many sites of action Arf is regulated by GAPs with similar primary structure, there are mechanistic differences among the GAPs revealed by mutagenesis of Arf. In current studies in the laboratory, we will molecularly define the mechanistic differences so that they may be exploited therapeutically. This work requires the ability to study single turnover kinetics and to examine individual steps within the reaction sequence. A stopped flow instrument is the only tool currently available for studying reactions in the necessary time scales (1 msec ? 10 sec) that allows for the monitoring of specific protein-protein, protein-lipid and GTP hydrolysis continuously in this time scale. The stopped-flow instrument has the following features that are required for the LCO?s research: 1) Motor Drive: A computer-controlled servo motor drive to provide precise and reproducible mixing of 2 or 3 solutions. The motor should be digitally controlled with 4096 counts per revolution with resolver feedback for precise positioning, have high torque, low inertia, brushless motor and high performance and a digital power supply to provide essentially instantaneous starts and stops of the drive syringes. 2) Syringe chamber and cell assembly: The syringe/cell assembly should be easily removed for maintenance or for changing of cell types. The syringe chamber is transparent and thermostatted by circulating water. 3) Valve position sensors: Optical sensors to detect the load and fire positions of the sample load valve to prevent incorrect operation so as to preserve precious samples. 4) Light Source: A water-cooled 150 watt Mercury-Xenon arc lamp with parabolic mirror, and computer-controlled monochromator to provide a maximum intensity, stable light source. 5) Optical fiber: A quartz optical fiber bundle to bring light from the exit slit of the monochromator to the sample cell. 6) Detectors: A UV-sensitive photomultiplier and a UV-enhanced for simultaneous measurement of fluorescence and absorbance and a second photomultiplier to simultaneous measure two wavelengths or for real time polarization anisotropy measurements. 7) Software: Stopped-Flow system software that runs under Windows XP?, providing precise and complete computer control of data collection, analysis and printing and export of data to other PC programs for data analysis and presentation. In addition software for computer simulation of complex kinetic processes and fitting of data directly to mechanism using nonlinear regression and global analysis to fit an entire data set to a single reaction pathway is required. 8) Dead Time: A dead time of less than 1 millisecond. 9) Sample Volumes: A reaction volume of 20 ?l or less. 10) Data Collection and Analysis: 413 kHz 16 bit A/D converter to provide fast and accurate data collection. 11) Double Mixing Experiments: Ability to perform double mixing experiments. PART II: Facts and reasons to Justify Other than Full and Open Competition Statutory authority FAR 13.106-1(b)(1) b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). Justification: The KinTek stopped-flow instrument is the only instrument that uses a servo motor, rather than a pneumatic drive or stepper motors, to drive mixing. The motor is long lasting and gives very reproducible mixing times. In addition the SF-2400 stopped-flow instrument is the only instrument that has these critical features that are required for the LCO?s research: a) dead time of less than 1 ms; b) the greatest sensitivity; c) smallest sample size, which is especially important when reagents are difficult and expensive to prepare; d) collection of up to 3 signals simultaneously; e) Titration module; and f) Shutter to minimize photobleaching.
- Record
- SN01119197-W 20060820/060818220545 (fbodaily.com)
- Source
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