SOURCES SOUGHT
66 -- Low Density Microarray Development
- Notice Date
- 5/29/2007
- Notice Type
- Sources Sought
- NAICS
- 541380
— Testing Laboratories
- Contracting Office
- Department of Health and Human Services, Center for Disease Control and Prevention, Procurement and Grants Office (Atlanta), 2920 Brandywine Road, Room 3000, Atlanta, GA, 30341-4146, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- Reference-Number-43237
- Response Due
- 6/8/2007
- Archive Date
- 6/11/2007
- Description
- This is a Sources Sought notice for a project to develop of a low density microarray chip used to detect the DNA and RNA of the Lyme disease spirochete in ticks. This notice is for purposes of obtaining competition sources within the market. This notice does not constitute a commitment by the Government nor will the Government pay for information solicited herein. No solicitation exists; however, the Government?s anticipated requirements are provided below. ***************************************************************** Statement of Work Requirements/Information: I. BACKGROUND The Lyme disease spirochete is transmitted by ticks in the genus Ixodes. Lyme disease is the most common vector borne disease in the United States, with >23,000 cases reported to CDC in 2002. On average, over 20,000 are reported to CDC each year. In order to identify regions where ticks carry the Lyme disease spirochete, rapid and effective tests are needed to screen ticks for spirochetal infection in order to alert the general public of the Lyme disease risk in their area of residence. Although a variety of tests are available to detect spirochetes in ticks, they suffer from high cost, false positive results, and false negative results. Microarray chip technology has been used with pathogens such as influenza virus to cheaply, rapidly, and efficiently detect the nucleic acids of this pathogen in clinical samples. Adaptations of this method for detecting spirochetes in ticks holds promise to assist public health workers combat the Lyme disease epidemic. II. PURPOSE The overall goal of the project is to develop a low density microarray chip that can be used to detect the DNA and RNA of the Lyme disease spirochete in ticks. The microarray chip should be both sensitive in detecting spirochetes that are actually present in these vector ticks, and specific in not producing false positive results by detecting other pathogens present in ticks. The microarray will be used in the field to detect spirochetes in field populations of ticks as well as ticks on individual patients. III. SCOPE OF WORK The vendor shall furnish all labor, materials, and equipment necessary to design a low density microarray that can detect spirochetes in ticks. Based on the known nucleic acid sequence of the Lyme disease spirochete, vendor will design nucleic acid probes that bind sensitively and specifically to the Lyme disease spirochete. Once the microarray is designed, the micoarray must be tested on known positive tick samples to determine the sensitivity and specificity of the test. The microarray will then be tested against field collected ticks to determine its efficiency. IV. TASKS TO BE PERFORMED The vendor shall perform the following tasks in the order shown: 1. Select candidate nucleic acid probes from the known genetic sequence of the Lyme disease spirochete to detect the DNA and RNA of this pathogen 2. Test low density microarray against known positive samples of Lyme disease spirochete nucleic acid. 3. Determine the sensitivity and specificity of the microarray chip against known positive nucleic acid samples. Achieve a sensitivity (positive tests/known positive samples) of >95%. Achieve a specificity (negative tests/known negative samples) of >98%. 4. Determine the sensitivity and specificity of the microarray chip on ticks known to be positive for the Lyme disease spirochete and ticks known to be negative for the Lyme disease spirochete. 5. Test the microarray chip on samples of field collected ticks from areas known to be hyperendemic for Lyme disease. 6. File a report to CDC describing the development of the assay and then provide data on the test results against ticks. ***************************************************************** Potential offerors who believe they are capable of providing the services referenced herein, shall submit all responses to this notice to the point of contact listed below. All responses shall be provided not later than 6/8/07 and shall be submitted via e-mail to: tdc0@cdc.gov. No telephone call will be expected, all inquiries must be in writing and provided as stated above. ##
- Place of Performance
- Address: Atlanta, GA
- Zip Code: 30341
- Country: UNITED STATES
- Zip Code: 30341
- Record
- SN01304500-W 20070531/070529220219 (fbodaily.com)
- Source
-
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