SOLICITATION NOTICE
66 -- Upright Spectral Confocal Microscope
- Notice Date
- 7/19/2007
- Notice Type
- Solicitation Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Agriculture, Agricultural Research Service, Pacific West Area Office, 800 Buchanan Street, Albany, CA, 94710-1105, UNITED STATES
- ZIP Code
- 00000
- Solicitation Number
- RFQ05-91H2-07
- Response Due
- 7/31/2007
- Archive Date
- 7/31/2007
- Description
- The USDA, Western Regional Research Center, Albany, California has a requirement for one (1) laser scanning confocal microscope, built around a complete DM 6000 upright bionocular base with optical outfit for upright microscope, complete fluorescence setup, SuperZ Galvo focusing stage, transmitted light detector, and configured with four objectives: HC PL FLUOTAR 10x0.30, HCX PL APO 20x/0.70 multi-immersion, CORR, HCX PL APO CS 40x/1.25-0.75, HCX PL APO 63x/1.20 W CORR CS, HCX PL APO 100x/1.40-0.70 Oil. Must be fully automated upright microscope with motorized focus, filter changer, condenser aperture and field diaphragm, completely integrated into confocal control. Must have interference contrast setup, SP5 scanner module with programmable beam splitter (AOBS), conventional SP5 scanning system, 3x SP detector channel, imaging laser RYB (Red 633nm/Yellow 561nm/Blue 453/488/514nm), exhaust with silencer for Ar laser. Laser port 405nm, imaging laser 405nm cw 50mW, AOTF for controlling 405nm laser intensity, UV correction optics, workstation with 2xLCD, and LAS AF SP5 offline workstation software. The spectral confocal microscope must be completely filter-free in the optical light path of the scanner, including programmable electronic beam splitter for unmatched flexibility in the use of excitation laser lines and increased sensitivity. Use of prism in spectral light path for highest transmission efficiency, up to 96%. Spectral detectors with use of motorized slits in front of each for wavelength selection. Motorized slits are fully computer-controlled for adjustment of the detection of the wavelength of fluorescence signal. Slits are controlled with nm accuracy and changeable during scanning for fine adjustment with a minimum width of 5nm. Scanner head provides exit port for access to confocal emission light path. User can adapt/couple their detection device to the port. Scanner must be upgradeable to be equipped with tandem device to increase scanning speed up to 16000 lines per second. Optional avalanche photo-diode (APDs) with 2 ? 4x higher quantum efficiency available for exit port attachment for low-light and live cell imaging. Scan rotation in any scan speed and in bi-directional mode without effecting scan speed or optical resolution, up to 200 degrees. Sequential scan in line, frame and stack modes. True region of interest (ROI) scan. Multiple ROIs can be designated, and different laser line for each ROI is possible. Imaging in 8/12/16 bit, up to 8k x 8k pixel format. Large scan field (FOV 22); with 10x objective, maximum field size is 1.5mm x 1.5mm. Optical zoom up to 32x. Single detection pinhole for perfect registration of confocal images from all detectors. Daily maintenance is not needed to ensure multi-color image registration. Scanner optics with chromatic aberration correction. Three visible lasers (expandable to 8 ? UV/405-vis-IR) coupled to scanner head, usable simultaneously. Available lasers are argon laser (458nm, 476nm, 488nm, 496nm, 514nm), yellow DPSS laser (561nm), and red HeNe laser (633nm). All laser lines intensity controlled by acousto-optical tunable filter and freely combinable. 405nm laser with AOTF intensity control for ROI scanning mode, plus optical correction for optimized merging with visible laser lines. Three spectral fluorescence detectors (expandable to 5) and one transmitted light detector can be installed. All detectors usable simultaneously and sensitivities individually adjusted on-line during scanning. All internal fluorescence detectors/electronics are water-cooled for low noise imaging. Multi-function digital panel box for direct control of scanning parameters. User-programmable, with LED display of functions. Galvanometer focusing Z stage with 40nm accuracy, travel range 500 micron, expandable to 1.5mm. Offline processing software package with 3d and 4d processing, spectral analysis, co-localization analysis, post FRET/FRAP analysis and general image processing. Funds are not currently available for this acquisition and award will be made subject to the availability of funds. Interested vendors are encouraged to contact Clarice Dixson, USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710 at 510-559-6027 or e-mail cdixson@pw.usda.gov no later than July 31, 2007.
- Place of Performance
- Address: US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Location Support Services, 800 Buchanan Street, Albany, California
- Zip Code: 94710-1105
- Country: UNITED STATES
- Zip Code: 94710-1105
- Record
- SN01346548-W 20070721/070720000805 (fbodaily.com)
- Source
-
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