SOLICITATION NOTICE
66 -- TIRF Microscope
- Notice Date
- 7/14/2008
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- RFQ-NCI-80117-MM
- Archive Date
- 8/7/2008
- Point of Contact
- Melissa P Marino, Phone: 301-402-4509, Caren N Rasmussen,, Phone: (301) 402-4509
- E-Mail Address
-
marinome@mail.nih.gov, cr214i@nih.gov
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI) plans to procure the brand name Nikon Instruments or equal Nikon Ti Microscope with TIRF optics. This acquisition will be conducted under the procedures for acquiring commercial items authorized in FAR Part 12 and under the authority to use simplified procedures for commercial requirements as provided in FAR 13.501. The North American Industry Classification System code is 334516 and the business size standard is 500 employees. Only one award will be made as a result of this solicitation. Contractor shall provide the Ti-E Inverted Microscope with TIRF optics, Dual-View split chip imaging ready for upgrade with photobleaching/photoactivation capabilities or equal. The following salient characteristics apply: 1)Fully automated selection of multiple excitation wavelengths (440, 488, 514, 561, 638) and emission wavelengths, 2)AOTF laser control, 3)Integrated high speed emission filter wheels, 4)Up to 4 TIRF angles can be set in memory, 5)Lens array (fly-eye lens) to provide uniform illumination of the visual field, 6)EMCCD detector (12 bit digital images of 1024 X 1024 pixels), backthinned chip with quantum efficiency 95%, cooled to at least -85°C, compatible with a possible upgrade to a 2 camera synchronized system, 7)All optical elements needed to acquire 2 simultaneous images with the EMCCD detector (Dual View split chip capabilities), Dual View controls must be fully integrated into the system software 8)Z-control with a feedback system to maintain focus that updates every 10 ms with offset height performed optically with linear encoder and offset lens, using an 870nm LED, 9)5 laser lines: 440 nm, 488 nm, 514nm, 561 nm and 638 nm, 10)Sequential image capture of fluorescent probes and viewing and recording of TIRF along with brightfield images, 11)USB controlled HUB for controlling up to 7 motorized accessories, 12)Video playback as single sections or maximum intensity projections in grayscale or pseudocolor, playback possible during image acquisition in a time series, 13)System ready to integrate photobleaching and photoactivation unit, including control of the photobleaching unit through the system software (Nikon Elements) 2Delivery: Contractor shall deliver the item within ninety (90) days after award to: NIH/NCI Bethesda, Maryland 20892. Upon award and delivery, contractor must notify the NCI Project Officer to schedule a delivery date and time. 3Installation: Contractor shall install the equipment and have ready to use within seven (7) days after delivery. 4 Training: Contractor shall provide training for use of equipment, software and related use for one (1) NCI researcher at above location within one week of installation. 5Payment:Payment shall be made after delivery and installation of equipment, upon Government acceptance Justification: Acquisition of a total internal reflection fluorescence (TIRF) microscope will enable more efficient performance of the work performed at NCI that is dependent on observation of microscopic structures. This microscope will allow the NCI researchers to observe structures near the cell surface with a "z" resolution beyond what is possible with normal optics. Furthermore since the rest of the cell is not illuminated, inference from fluorescent molecules inside the cell is greatly reduced, allowing visualization of faint surface features that cannot be seen with other instruments. In order to study the structures involved in cell adhesion, NCI must also distinguish the cell surface from the immediate area inside the cell, which cannot be done with a confocal microscope. NCI needs to observe protein translocation in the social amoeba, Dictyostelium discoideum, as important molecules pass through the plasma membrane. Dictyostelium discoideum cells are easily damaged by light so the limited light penetration of a TIRF instrument is ideal for this organism. NCI is also studying the formation and movement of T cell receptor complexes in activated T-cells. To separate the surface molecules from those that have been internalized NCI needs "z" resolution in the range of the diameter of an endocytic vesicle, about 100nm. This system will also be used to study protein-protein interactions using Forester Resonance Energy Transfer (FRET) imaging with the high "z" resolution of the TIRF instrument in all of these systems. In order to acquire FRET images, we need to be able to capture 2 images simultaneously. In the future, this TIRF microscope will be upgraded to allow photobleaching and photoactivation studies. Potential offerors may request a copy of the solicitation from Melissa Marino, Contract Specialist via electronic mail at marinome@mail.nih.gov or fax (301) 402-4513. If you have questions regarding this notice, they may be addressed to the aforementioned individual noted above. Estimated issued date of the solicitation is July 29, 2008 with an estimated due date of August 12, 2008. Interested parties must provide a point of contact: name, phone number, fax number, email address, complete mailing address, business size standard, and business name. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA). Reference: RFQ-NCI-80117-MM on all correspondence.
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- Record
- SN01613757-W 20080716/080714223357-1f084c2a070b1309f8daf90a54be59db (fbodaily.com)
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