SOLICITATION NOTICE
66 -- Benchtop MALDI-TOF Mass Spectrometer
- Notice Date
- 7/17/2008
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Agriculture, Agricultural Research Service, Pacific West Area Office, 800 Buchanan Street, Albany, California, 94710-1105
- ZIP Code
- 94710-1105
- Solicitation Number
- PSM-24-08
- Archive Date
- 8/8/2008
- Point of Contact
- Clarice E. Dixson,, Phone: 510-559-6027, Clarice E. Dixson,, Phone: 510-559-6027
- E-Mail Address
-
clarice.dixson@ars.usda.gov, clarice.dixson@ars.usda.gov
- Small Business Set-Aside
- N/A
- Description
- BenchtopMatrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer (MALDI-TOF-MS) The USDA, ARS, Western Regional Research Center, Albany, California has a requirement for a state-of-the-art benchtop matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) with a vertical flight tube. The instrument must operate in linear mode only and be capable of MS analysis of biomolecules up to 300,000 m/z. The instrument shall operate in both positive and negative ion mode with an acceleration voltage of  20 kV. A pulsed nitrogen laser with a repetition rate of up to 20 Hz will be provided. A PC-based computer will operate the instrument as well as acquire and analyze data. This computer must allow efficient data archiving by data transfer using CD/DVD "burner" or with a memory stick from a USB port. Software shall be provided for the rapid identification of microorganisms (and dendrogram tree relationships) by analysis of MS spectral similarity between unknown strains and known strains from a MS reference library that will also be included with the instrument. The instrument and associated software must have at least a 1-year warranty. I. Mass Spectrometer Hardware Requirements A. Laser 1. Instrument to be equipped with a pulsed nitrogen laser with a wavelength of 337 nm. 2. Laser to have a variable repetition rate of 1-20 Hz. 3. Pulse energy per pulse of > 150 J. B. Optical system 1. An attenuator will vary the intensity of the laser at the sample stage continuously over the full power range of the laser. 2. The UV optics will focus, size and position the laser beam on the sample stage and must not vary significantly as illumination is changed over the full range. C. Sample Introduction System 1. 1/4 microtiter plate design. 2. X-Y target positioning controlled by PC-mouse. D. Sample Viewing System 1. Vendor will supply as part of basic instrument a video system for viewing the sample surface at sufficient magnification to assess the effect of sample surface morphology on spectral results. 2. Viewing system must indicate the point of laser impact. E. Ion Source 1. A gridless ion source with delayed ion extraction/acceleration to +20/-20 kV. 2. Ion source power supplies must be extremely reliable and stable. F. Analyzers 1. MS Linear a. Linear path length at least 105 cm b. Resolution > 2500 (Bombesin, 1619.8) FWHM c. Sensitivity: 1 fmol[Glu1]-Fibrinopeptide B (m/z 1,570.7) at S/N > 10:1 d. Mass accuracy: < 200 ppm with external calibration < 75 ppm with internal calibration e. MALDI mass range up to 300,000 m/z. G. Detection System 1. An electron multiplier detector that will not saturate due to matrix desorption. 2. Ultra-stable power supplies for detectors. 3. 500 MHz digitizer H. Vacuum System 1. At least one turbomolecular pump capable of providing high vacuum consistent with collision-free conditions for ions traveling from the source to the detector. 2. Turbomolecular pump backed with high performance/low maintenance vacuum pump. 3. A series of redundant vacuum gauges to continuously monitor vacuum pressure and interlocked to high voltage power supplies in the case of loss of vacuum integrity. II Data System Requirements A. Hardware 1. A PC-based computer workstation, > 3.2 GHz dual-Xeon-processor, 1 GByte RAM, 160 GByte hard disk, DVD+R/RW drive, DVD-ROM drive, 1x Ethernet connection for external networks, 500 MHz Digitizer plug-in card; > 20" LCD-Display. 2. All instrument operating conditions, raw spectral data, and mass spectral peak lists are stored in a database accessible through the instrument control software. 3. A local area network (LAN) for remote access data acquisition and data review will be provided. B. Software General: Vendor must supply a fully integrated PC-based software for the operation of the instrument as well as the acquisition and analysis of data. 1. Data acquisition a. Real time display of spectrum for each laser shot during acquisition. b. Display of either time or mass-assigned data. c. Automated instrument re-calibration routines. d. Automated data acquisition for entire MALDI plate target. 2. Instrument optimization software a. Software to provide for adjustment of all relevant instrument parameters. b. Resolution optimization system to be available. c. Auto-tuning: the system can be queued to acquire and automatically update voltages for all user accessible tuning parameters. 3. Data Analysis Software a. Post-run manipulation of raw data to be possible, including peak picking, background subtraction, smoothing and mass assignment using different calibrations to be provided. 4. Microorganism identification software a. Software must be able to be able to rapidly compare and group MS spectra of different microorganisms based on spectral similarity. This should be accomplished using sophisticated pattern recognition algorithms and/or cluster analysis algorithms. The display of the analysis should include identification of dendrogram tree relationships between different microorganisms of strains of known and unknown origin. b. Microorganism identification software must seamlessly and efficiently interface with MS data acquisition software. c. Automated data comparisons immediately after spectral acquisition. d. Data comparison allows selective weighting of ion mass-to-charge (m/z) or range of m/z for discriminating between closely related microorganisms, e.g. sub-speciation. 5. A reference library of over 3000 MALDI-TOF-MS spectra from known microorganism strains from well characterized strain collections. This solicitation incorporates the following commercial FAR clauses by reference 52.212-1, 52.212-2, 52.212-3, 52.212-4, and 52.212-5. Funds are not currently available for this acquisition and award will be made subject to the availability of funds. Interested vendors are encouraged to submit proposals containing descriptive literature of their products to Clarice Dixson, USDA, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710 or by e-mail to Clarice.Dixson@ars.usda.gov no later than COB August 7, 2008.
- Web Link
-
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- Place of Performance
- Address: USDA, Agricultural Research Center, Western Regional Research Center, 800 Buchanan Street, Albany, California, 94710, United States
- Zip Code: 94710
- Zip Code: 94710
- Record
- SN01616132-W 20080719/080717220010-bfc00507161a5ea1c4e815ec490e8e45 (fbodaily.com)
- Source
-
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