SOLICITATION NOTICE
66 -- Optical Instrument and Lens
- Notice Date
- 7/29/2008
- Notice Type
- Presolicitation
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-PB-AR-2008-214-RCO
- Archive Date
- 8/27/2008
- Point of Contact
- Rosie C Owens,, Phone: (301) 435-7920, Kathleen Marsden,, Phone: 301.435.0364
- E-Mail Address
-
rowens@mail.nih.gov, marsdenk@nhlbi.nih.gov
- Small Business Set-Aside
- N/A
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NHLBI-PB-(AR)-2008-214-RCO and is issued as a Request for Quotation (RFQ). The solicitation will include all applicable provisions and clauses in effect through Federal Acquisition Circular 05-26 (as of 06/12/2008). The North American Industry Classification (NAICS) Code is 333314, and the business size standard is 500. This acquisition is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. However, this solicitation is set aside for small businesses. It is the intent of the National Heart, Lung, and Blood Institute’s (NHLBI) Consolidated Operations Acquisition Centers (COAC) on behalf of National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) to procure the services from Leica Microsystems Inc., 2345 Waukegan Road, Suite 200, Bannockburn, IL 60015, for the following product and service: a) Total Internal Reflection Fluorescence (TIRF) no. 11888383, b) Multi Colour Box w/ 405nm no.11888364, c) Leica Workstation D965/6400 AF6000 o.SW no. 11600247, d) Obj. HCX PL APO 40x/1.25-0.75 oil, 0.17/D no. 11506250 Summary: The above laboratory equipment is essential to NIAMS’s continued research efforts: Background Research in the Section focuses on the cell biology of skeletal muscle. Skeletal muscle is made of uniquely organized large multinucleated cells, built through successive developmental changes in cell morphology and subcellular organization. We are currently particularly interested in the organization of the Golgi complex (GC). One of the important functions of the GC is the targeting of membrane proteins. This is an essential function in a cell with distinct membrane domains. Muscle fibers have several membrane domains: the plasma membrane, the T-tubules, the neuromuscular junction, the myotendinous junctions, to which proteins must be specifically targeted. Some of these domains extend at large distances from the nuclei. Instead of compact Golgi complexes associated with the nuclei, muscle fibers have thousands of small Golgi complexes dispersed throughout the fibers. We have shown that their distribution is fiber type-specific and regulated by the patterned contractile activity of the muscle fiber. Although the organization of the GC in muscle appears specific to this tissue, we have shown that it relies on principles common to all mammalian cells linking the Golgi complex, the microtubule cytoskeleton and the endoplasmic reticulum (ER). Our present work focuses on understanding how the GC changes are regulated during muscle development and are caused by contractile activity. Experiments in progress include the relative localization of GC, ER exit sites and centrosomal proteins by immunofluorescence, immunogold EM, and by observation of GFP-tagged proteins in live cells, as well as investigations of the signaling pathway(s) involved. We are currently investigating whether microtubule stabilization, which takes place during muscle differentiation, plays a role in the relocation of the GC. We are also using transgenic mice lacking cytoskeletal proteins to probe their role. As part of our research on muscle cytoskeleton, a single fiber from a mouse Tibialis Anterior muscle was stained with antibodies against the proteins syne (cytoskeleton) and caveolin-3 (cell surface), and counterstained with Hoechst, a fluorescent dye that labels nuclei. The fiber was photographed in the Zeiss LSM510 confocal microscope. This image is the superposition of a transmitted light image of the fiber (white) and of the immunofluorescence images (red for syne, green for caveolin-3, blue for the nuclei). The blood vessels that surrounded the muscle fiber in the muscle have been removed but have left ditches that appear as sinuous red channels. Ralston E, Swaim B, Czapiga M, Hwu WL, Chien YH, Pittis MG, Bembi B, Schwartz O, Plotz P, Raben N. Detection and imaging of non-contractile inclusions and sarcomeric anomalies in skeletal muscle by second harmonic generation combined with two-photon excited fluorescence. J Struct Biol. 2008 Jun;162(3):500-8. Epub 2008 Apr 8. Ralston, E., Lu Z., Biscocho, N., Soumaka, E., Mavroidis, M., Prats, C., Lømo, T., Capetanaki, Y. & Ploug, T. Blood vessels and desmin control the positioning of nuclei in skeletal muscle fibers. J. Cell. Physiol. Published Online: 13 Sep 2006 
DOI: 10.1002/jcp.20780. Bugnard E, Zaal KJ, Ralston E. Reorganization of microtubule nucleation during muscle differentiation. Cell Motil Cytoskeleton. 2005; 60(1): 1-13. Ralston, E., Ploug, T., Kalhovde, J., and Lømo, T. Golgi complex, ER exit sites and microtubules in skeletal muscle fibers are organized by patterned activity. J. Neurosci. 2001; 21, 875-883. The sole source determination is based upon the fact that Leica Microsystems Inc., is the manufacturer of the DMI6000B Microscope and the TIRF MC Retrofi Kit 3 color must be compatible to fit existing microscope. The TIRF is the only one in which orientation of the light beam is calculated automatically. Also, the TIRF is the only source with 50 to 250nm penetration of light beam angle. Leica is the only known system that meets the Government’s minimum needs. Delivery date: Thirty days (30) after receipt of purchase order The offeror must include a completed copy of the following provisions: 1) FAR Clause 52.212-1 Instructions to Offerors Commercial; 2) FAR Clause 52.212-2, Evaluation Commercial Items. As stated in FAR Clause 52.212-2 (a), The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: Technical Evaluation and Price. 3) FAR Clause 52.212-3, Offeror Representations and Certifications Commercial Items; 4) FAR Clause 52.212-4, Contract Terms and Conditions Required To Implement Statues or Executive Orders Commercial Items, Contract Terms and Conditions Commercial Items; and 5) FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders Commercial Items Deviation for Simplified Acquisitions. This action is under the authority of 41 U.S.C. 253(c) (1), as set forth in FAR 6.302-1 and HHSAR 306-302-1. Only one responsible source and no other supplies or services will satisfy agency requirement. This notice of intent is not a request for competitive quotations however; all responses received within 15 days from the date of publication of this synopsis will be considered by the Government. Interested parties may identify their interest and capability to respond to the requirement or submit proposals. However, the determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. The offeror must include in their quotation, the unit price, the list price, shipping and handling cost. Note: In order to receive an award from the NHLBI contractors must have a valid registration in the Central Contractor Registration (CCR) www.ccr.gov and registered in Dun and Bradstreet (DUNS), the Taxpayer Identification and the certification of business size www.bpn.gov. The clauses are available in full text at http://www.arnet.gov/far. Quotations will be due fifteen (15) calendar days from the publication date of this synopsis or by August 12, 2008, 5pm, Eastern Standard Time. Responses will only be accepted if dated and signed by an authorized company representative. Interested vendors capable of furnishing the government with the item specified in this synopsis should submit their quotation to the below address. Inquires to this notice, referencing number NHLBI-PB-(AR)-2008-214-RCO, submitted in writing to the National Heart, Lung and Blood Institute, Office of Acquisition, Procurement Branch, 6701 Rockledge Drive, Suite 6128, Bethesda, Maryland 20892-7902. Attention: Ms. Rosie C. Owens, Contract Specialist. If submitted electronically: Email: Rosie C. Owens, Contract Specialist rowens@nhlbi.nih.gov or Ms. Kathleen Marsden, Contracting Officer marsdenk@nhlbi.nih.gov. Faxed copies will not be accepted.
- Web Link
-
FedBizOpps Complete View
(https://www.fbo.gov/?s=opportunity&mode=form&id=38156c103cf0d0df24c31ed2487d36ea&tab=core&_cview=1)
- Place of Performance
- Address: Bethesda, Maryland 20892-0001, Bethesda, Maryland, 20892-0001, United States
- Zip Code: 20892-0001
- Zip Code: 20892-0001
- Record
- SN01625198-W 20080731/080729223109-38156c103cf0d0df24c31ed2487d36ea (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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