SOLICITATION NOTICE
66 -- SRM 968e, Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Frozen Human Serum
- Notice Date
- 8/13/2008
- Notice Type
- Modification/Amendment
- NAICS
- 325414
— Biological Product (except Diagnostic) Manufacturing
- Contracting Office
- Department of Commerce, National Institute of Standards and Technology (NIST), Acquisition Management Division, 100 Bureau Drive, Building 301, Room B129, Mail Stop 1640, Gaithersburg, Maryland, 20899-1640
- ZIP Code
- 20899-1640
- Solicitation Number
- SB1341-08-RQ-0552
- Response Due
- 8/25/2008 3:00:00 PM
- Archive Date
- 9/9/2008
- Point of Contact
- Andrea A Parekh, Phone: (301)975-6984, Todd D Hill,, Phone: 301-975-8802
- E-Mail Address
-
andrea.parekh@nist.gov, todd.hill@nist.gov
- Small Business Set-Aside
- Total Small Business
- Description
- The following are responses to questions received in regards to the subject solicitation: Question 1. Since the ultimate goal is to have serum, why not produce off the clot serum (or better serum produced by CLSI C37-A) and add the NIST additives to this? The number of analytes listed for SRM 968 (cholesterol, retinol, retinyl palmitate, alpha tocopherol, trans beta carotene, total beta carotene (trans plus cis isomers), total alpha carotene and lutein as well as non-certified values for tocopherol (includes beta tocopherol, delta tocopherol, zeaxanthin, beta cryptoxanthin, trans-lycopene, trans alpha carotene, total lycopene, 9 cis carotene, 13 plus 15 cis carotene and 15 cis carotene) would mandate caution in how much manipulation of the source material is advisable. All procedures to convert plasma to serum represent potential matrix effects that could cause variation in analysis among different systems. This is certainly true at least for cholesterol as demonstrated by the lipid standardization program through the CDC among many other programs. Answer 1. NIST produced SRMs 968, 968a, 968b, and 968c in house. Plasma was converted to serum through freeze-thaw cycles and filtration and we have not had commutability issues. The question raises a good point in that some matrix effects have been seen in SRM 968d, perhaps as a result of the method used for converting the plasma to serum, which was through the addition of bovine thrombin and calcium chloride followed by dialysis and the addition of salts. Question 2. If plasma must be the starting material, what is the method or chemical used to defeat clotting? This is probably citrate, but if not it would be helpful to know for any conversion to serum. Answer 2. Yes, 4% sodium citrate is added. Question 3. There are a number of ways to convert citrated plasma to serum, the most common being to add calcium to clotting levels. However, since this is an SRM is there not a standard procedure that the NIST wishes to follow to mimic as closely as possible the preceding SRMs? If so what is it? Answer 3. We thaw the frozen plasma and vacuum filter through Whatman 541 filter paper (or Gelman P1 QUAL MED FLW (part no. 61000), blend and spike if necessary, equilibrate overnight, vacuum filter again as above, and bottle
- Web Link
-
FedBizOpps Complete View
(https://www.fbo.gov/?s=opportunity&mode=form&id=aebb9297e7ea50b6afe0fa5dc9485118&tab=core&_cview=1)
- Place of Performance
- Address: 100 Bureau Drive, MS 1640, Gaithersburg, Maryland, 20899, United States
- Zip Code: 20899
- Zip Code: 20899
- Record
- SN01639168-W 20080815/080813223323-aebb9297e7ea50b6afe0fa5dc9485118 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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