SOURCES SOUGHT
66 -- Applied Biosystems TaqMan MicroRNA Arrays
- Notice Date
- 8/14/2009
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- SS-90180-2009-MM
- Archive Date
- 9/8/2009
- Point of Contact
- Melissa P Marino, Phone: 301-402-4509, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
marinome@mail.nih.gov, cr214i@nih.gov
(marinome@mail.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- RECOVERY SOURCES SOUGHT NOTICE: TaqMan Human microRNA Array A & B Notice Number: SS-90180-2009-MM Issued By: National Cancer Institute (NCI), (http://www.nci.nih.gov) Office of Acquisitions (OA) Key Dates: Capability Statement Due Date: August 24, 2009 11AM EST. General Information: This Sources Sought Notice (SS) is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Cancer Institute (NCI). Purpose: The purpose of this Sources Sought Notice is to identify qualified small business concerns including 8(a), HUBZone or Service-Disabled Veteran-owned business concerns that are interested in and capable of providing the equipment described herein. The NCI does not intend to award a contract on the basis of responses received nor otherwise pay for the preparation of any information submitted. As a result of this Sources Sought Notice, the NCI may issue a Request for Quotation (RFQ). THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NCI shall arise as a result of a response to this Sources Sought Notice or the NCI's use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. Description of Information to be included in the Capability Statement: Contractor Requirements: Equipment capabilities and facility requirements Specifically, information is sought regarding equipment with the following salient characteristics: Unique features of the Applied Biosystems TaqMan MicroRNA Arrays include: 1.)Reaction vessel uses microfluidic technology in a 384-well plate format. 2.)The reaction vessel has 8 sample loading ports and must use centrifugation to distribute the samples into each of the 48 wells serviced by each port. 3.)The reaction vessel is compatible with the Applied Biosystems 7900HT Sequence Detection System (a real-time PCR instrument). NCI already has this system in the lab, so there is no need to purchase additional equipment. 4.)The reaction vessel is pre-loaded with pre-designed 5'-nuclease assays using TaqMan® probes. a. The assay contains the necessary primers and probes to perform both reverse transcription of the targeted miRNA, and real time PCR detection of that miRNA transcript only. b. The assay id specific for the mature form of the miRNA transcript. c.The real time PCR detection method includes TaqMan® probes (5' nuclease chemistry) with MGB quenching the 3' end of the probe. d.The TaqMan® probe is cleaved when the probe hybridizes to the target. e.The assay has a minimum dynamic range of 6 logs. 5.)The vendor shall supply all the necessary consumables to perform real-time quantitative PCR, including PCR reagents designed for use with the fluorogenic 5' nuclease assay. All PCR reagents contain a passive internal reference dye to minimize well-to-well variability. 6.)The vendor shall provide comprehensive assay design and development guidelines for real-time quantitative PCR. 7.)The vendor shall offer Telephone Technical Support and Field Applications/Sales/Service Support to help solve chemistry and instrumentation problems encountered with real-time quantitative PCR. 8.)High specificity: single base pair discrimination of mature miRNA. 9.)Sensitivity: single copy of target 10.)Linear Dynamic Range: 7-9 logs of linear dynamic range 11.)Ease of use: Easily implementable on existing 7900HT, with only 2 steps that take approximately 3-10 minutes total hands on time average per sample when operating in high throughput (15-30 minutes per sample hands on time in low throughput) 12.)Low sample input: Ability to assess single cells or low cell population (Non-biased assessment of single cells with the TaqMan Array). Ability to do Laser Capture Microdissection (LCM), FFPE (Formalin Fixed Paraffin Embedded) tissues, and every other tissue with minimally 1 cell and no limitation on abundant sample. 13.)Highly parallel miRNA profiling/detection in the TaqMan Array Cards (TLDA) with TaqMan miRNA Gold Standard Assays NAICS Code and Size Standard: In the event an RFQ is issued, North American Industry Classification System (NAICS) code 334516 with a size standard of 500 employees is being considered. How to Submit a Response: Page Limitations: Interested qualified small business organizations should submit a tailored capability statement for this requirement not to exceed 10 single sided pages including all attachments, resumes, charts, etc. (single space, 12 point font minimum) that clearly details the ability to perform the aspects of the notice described above. All proprietary information should be marked as such. Statements should also include an indication of current certified small business status; this indication should be clearly marked on the first page of your capability statement (preferable placed under the eligible small business concern's name and address). Responses will be reviewed only by NCI personnel and will be held in a confidential manner. Due Date: Capability statements are due no later than 11a.m. August 24, 2009. Number of Copies and Delivery Point: Please submit one (1) original and three (3) copies of your response as follows: If hand-delivered or delivery service:If using U.S. Postal Service Melissa Marino - Contact SpecialistMelissa Marino - Contract Specialist 6120 Executive Boulevard, Room 60706120 Executive Boulevard, Room 6070 Rockville, MD 20852-7194Rockville, MD 20852-7194 All capability statements sent in response to the Sources Sought Notice must be submitted in writing to Melissa Marino. All responses must be received at NCI by the specified due date and time in order to be considered. No faxed or electronic mail submissions will be accepted. Point of Contact: Inquiries concerning this Notice may be direct to: Melissa Marino Contract Specialist 6120 Executive Boulevard, Room 6070 Rockville, MD 20852-7194 marinome@mail.nih.gov
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/SS-90180-2009-MM/listing.html)
- Record
- SN01912158-W 20090816/090815001849-b69cc5acbb079ef5900ad6b9b155b604 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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