SOLICITATION NOTICE
66 -- Qiagen - RotorGene Q 5flex HRM real-time PCR system and QIAgility Liquid Handling System
- Notice Date
- 8/31/2009
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- NCI-90222-NG
- Archive Date
- 9/30/2009
- Point of Contact
- Malinda L Holdcraft, Phone: (301) 402-4509, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
holdcram@exchange.nih.gov, cr214i@nih.gov
(holdcram@exchange.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Pathology (LP) plans to procure as a sole source the brand name Qiagen - RotorGene Q 5flex HRM real-time PCR system and QIAgility Liquid Handling System from Qiagen, Inc., 27220 Turnberry Lane, Suite 200, Valencia, CA 91355. The services herein are being procured in accordance with the simplified acquisition procedures authorized by FAR Part 13.106-1 (b)(1). The North American Industry Classification System Code is 334516 and the business size standard is 500 employees. Delivery shall be made within four to six weeks after award. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI to determine if the party can meet this requirement. One (1) original and one (1) copy of the capability statement must be received in the contracting office by 11:00 a.m. ET on September 15, 2009. All questions must be in writing and can be faxed to 301-402-4513 or sent via email to Malinda Holdcraft: holdcram@exchange.nih.gov. It is the vendor’s responsibility to ensure questions have been received. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractor must have valid registration and certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Application (ORCA). Please reference NCI-90222-NG on all correspondence. Part I: Background information and description of requirements: The Molecular Diagnostics Laboratory, Laboratory of Pathology performs molecular testing to assist in the diagnosis of a variety of cancers. It is currently the only CLIA and College of American Pathology approved clinical lab within the NCI certified for performing molecular oncology testing on materials from NIH patients. The use of PCR and in particular real-time PCR for cancer testing has multiple applications in the molecular diagnostics laboratory for the detection of clonality, chromosomal translocations, minimal residual disease detection, disease associated polymorphisms, and gene expression signatures that correlate with diagnosis and prognosis. The development of new tests requires the rapid evaluation of hundreds of primer sets. To facilitate efficient development of relevant primer sets, perform clinical laboratory testing, including melting temperature analysis, for the various purposes described above, the Molecular Diagnostics Laboratory requires a real-time PCR machine. JUSTIFICATION FOR OTHER THAN FULL AND OPEN COMPETITION Part II: Facts and Reasons to Justify Other than Full and Open Competition: Statutory Authority Statute: This justification has been prepared under simplified acquisition procedures as set forth in 13.106-1(b)(1). Justification The platform that best fits the needs of the Molecular Diagnostics Laboratory, Laboratory of Pathology, NCI is the RotorGeneQ real-time PCR instrument. In addition, to accommodate the higher volume of primer testing and molecular diagnostics tests performed by the laboratory, the laboratory requires a robotic platform that can perform set up and dilutions of the PCR reactions, and be compatible with the RotorGeneQ real time PCR instrument and several other platforms already in the laboratory. Qiagen has developed the QIAgility Robotic Liquid handler to interface with the RotorGeneQ and it is the only instrument that has this flexible capability. The purchase of these instruments will greatly increase the efficiency and speed of the NCI clinical laboratory test development, with resultant benefit to patient care. The following salient characteristics apply to the RotorGene Q 5flex HRM real-time PCR system, and QIAgility Liquid Handling System: 1. The real-time PCR system shall be capable of high resolution analysis for mutation detection High Resolution Melting Option- system must be capable of H.R.M. analysis allowing for mutation detection and resolution of single nucleotide polymorphisms (SNP’s) using high saturation intercalating dyes. 2. The real-time PCR system shall be capable of analyzing DNA association (annealing), dissociation (melting) and heteroduplex formation. 3. The real-time PCR system shall be designed with a centrifugal format allowing for samples to spin in a rotor at 400 rpm during heating and 400 rpm during cooling. 4. The real-time PCR system must allow for a uniform thermal chamber for heating and cooling of samples with a temperature uniformity specification of 0.01ºC. 5. The real-time PCR system must be a completely open platform capable of running all real-time chemistries (i.e. intercalating dyes, dual-labeled probes, FRET/quenched FRET probes, beacons, Eclipse probes) and be capable of multiplexing up to 6 dyes in a single tube (channel for H.R.M. should be an available option). 6. The system shall include up to 6 individual high power LED’s for excitation at 365nm, 470nm, 530nm, 585nm, 625nm and 670nm. 7. The system shall include 6 detection filters with wavelengths of 470nm, 510nm, 555nm, 610nm, 660nm, and 710nm. 8. The dimensions for the instrument shall be no larger than 370mm(w) x 420mm(d) x 275mm(h) and weigh no more than 14 kg. 9. The heating and cooling rate shall be at least 2.5ºC/sec for the tube temperature and 10.0ºC/sec for the air temperature. 10. Samples must be able to cycle in a standard.2ml PCR tube in a 36 place rotor or a 1ml tube in a 72 place rotor allowing for a 5ul total reaction volume. 11. HTS consumables must be available for 72 and 100 well applications with robotics integration. 12. Software must be easily upgradeable via the internet, free of charge, without any licensing fees associated, and no limit to the end user 13. The controlling computer and instrument must communicate via a serial RS-232 connection or USB 2.0 14. The software shall include wizards for automated programming and ease of use 15. The software shall include a text sensitive help menu program accessible from any part of the software with detailed explanations for each section. 16. The software shall allow for the following types of data analysis: real-time raw data in all available channels, real-time normalized data in all available channels, comparative quantitation analysis, auto-find threshold function, allelic discrimination analysis, melting curve analysis with genotyping definitions, comparative quantitation, 2 standard curves relative quantitation, LinReg, endpoint analysis, scatter plot, delta delta CT, Relative Expression Software Tool (REST), concentration analysis and H.R.M. 17. Sample IDs and analysis results must be exportable to excel and all graphs must be exportable to.jpeg and.bmp files 18. Reporting functions must be available for all analysis options and able to be emailed, saved to disc, sent to word, or printed from the same window 19. The real-time PCR system must allow for remote temperature calibration with a unique TLC calibration rotor and results should be analyzable through the use of e-mailed or internet accessible reporting. 20. Optical denaturation must be an option available to the end user for the optimization of reactions. The robotic component shall be fully compatible with the RotorGene Q 5flex HRM and must have the following features: 1. The workstation shall be specifically designed for PCR and QPCR applications 2. The workstation shall have the capability to normalize final template concentrations based on importing data directly from LIMS/Excel systems and setup intermediate dilutions plates if needed 3. The workstation shall have the capability to prepare at least four separate master mixes in a single run 4. The workstation shall have an integrated UV lamp and HEPA filter preferable in the hood of the robot 5. The workstation shall incorporate an integrated hood to protect pipetting arm during movement and facilitate a controlled and shielded environment. 6. The workstation Pipettor shall be designed with a helical lead screw to allow for minimal dead air volume and the dead air volume must be adjustable within the software. 7. The software must be easily upgradeable, free of charge, without any licensing fees associated. 8. The Pipettor must have liquid level sensing through the use of conductive tips but must also run with non-conductive tips. 9. The unit must have a small foot print, no larger than 533x626x443 (mm, WxDxH). 10. The pipetting range shall be 1ul-200ul with C.V. < 1% at volumes  5ul. 11. The robot must communicate with laptop or desktop computer via serial port RS-232. 12. The robot must allow for pipetting into both standard formats 96 and 384 well plates as well as allow for pipetting into non-standard formats to include 0.1ml 72 position block for Rotor-Gene Q™ Real-Time PCR System, Gene Disc 100 and 72, glass capillaries for Roche Light Cycler, Cepheid Smart Cycler tubes, Roche COBAS system and other preset plate definition files 13. The software must generate a diagnostic email report and bundle relevant files for technical assistance with a single mouse click. 14. The Pipettor must have multi-eject function for repeat pipetting, and tips should be ejected outside of the machine. 15. The software must be able to support commonly used qPCR and Forensic STR-PCR reaction setup protocols. 16. The robot must have auto height calibration function to set Z height and use Z-head retraction while dispensing. 17. The software must support setup wizards for ease of use in programming such as automatic standard curve dilution series creation varied from 1:2 to 1:10 fold dilution. 18. The tube/reagent racks must be made of aluminum and be removable for storage and refrigeration. 19. The software must be able to import sample names easily from any existing sample management system and export sample information to any real-time thermal cycling system.
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-
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- Record
- SN01934455-W 20090902/090901002300-fba3ca21bf07d105cba0313e57f79734 (fbodaily.com)
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