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FBO DAILY ISSUE OF SEPTEMBER 02, 2009 FBO #2839
SOLICITATION NOTICE

66 -- Hi-SKY Complete Cytogenetic Imaging System

Notice Date
8/31/2009
 
Notice Type
Presolicitation
 
NAICS
334510 — Electromedical and Electrotherapeutic Apparatus Manufacturing
 
Contracting Office
Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
 
ZIP Code
20852
 
Solicitation Number
NCI-90121-MM
 
Archive Date
9/29/2009
 
Point of Contact
Melissa P Marino, Phone: 301-402-4509, Caren N Rasmussen, Phone: (301) 402-4509
 
E-Mail Address
marinome@mail.nih.gov, cr214i@nih.gov
(marinome@mail.nih.gov, cr214i@nih.gov)
 
Small Business Set-Aside
N/A
 
Description
The National Cancer Institute (NCI), Center for Cancer Research (CCR), plans to procure on a sole source basis the brand name Hi-SKY Complete Cytogenetic Imaging System with Applied Spectral Imaging, Inc. (ASI), 1497 Poinsettia Ave., Suite 158, Vista, CA 92081. The North American Industry Classification System Code is 334510 and the business size standard is 500 employees. This acquisition will be processed in accordance with simplified acquisition procedures as stated in FAR Part 13.106-1(b)(1). Delivery shall be in 4-6 weeks upon receipt of purchase order. The purpose of this acquisition is to procure and install a Hi-SKY system for the specific purpose of performing Spectral Karyotyping (SKY) analyses and development of Fluorescencee in situ hybridization (FISH) diagnostics to be performed on samples from metaphase chromosomes, touch preparations from frozen tissue samples, as well as sections from frozen/paraffin embedded tissue samples and obtained from cancer patients. Facts and Reasons to Justify Other than Full and Open Competition: 1.Statutory authority: Statute: This justification has been prepared under the authority of 41 U.S.C. 253 (c)(1) as set forth in 13.106-1 (b)(1). (b) Soliciting from a single source. (1) For purchases not exceeding the simplified acquisition threshold, contracting officers may solicit from one source if the contracting officer determines that the circumstances of the contract action deem only one source reasonably available (e.g., urgency, exclusive licensing agreements, or industrial mobilization). No other requirement and/or type for supply or service will satisfy agency need. Applied Spectral Imaging's HiSKY (spectral karyotyping) is proprietary to ASI. The interferometer optical head is only offered by Applied Spectral Imaging. The DNA Spectral Karyotyping Reagents are designed to enable simultaneous visualization of all chromosomes in different colors combinations across the full spectral range. The distinction between the dyes can be performed only with the SKY spectral imaging system from Applied Spectral Imaging. The unique protocol is intended for hybridization of the Spectral Karyotyping Reagents on a normal metaphase slide preparation. The salient characterstics that apply to the ASI Hi-SKY Complete Cytogenetic Imaging System are: 1.Hi-SKY stands for High Resolution Spectral Karyotyping. It is based on a unique electro-optical hardware that enables measuring the spectral image of chromosomes from a metaphase spread, previously stained, such that each chromosome has a different color (created typically from different combinations of 5 or more basic fluorescent dyes). While the staining scheme of multicolor-FISH (m-FISH) is identical, SKY's measurement scheme is very different than the one used in m-FISH, which is basically standard FISH acquisition done using multiple microscope filters. 2.All dyes are excited and acquired simultaneously using a single triple band filter cube using single focusing. 3.Hi-SKY achieves good Signal to Noise Ratio. Typical measurement time is 40-60 seconds; however, even 15 seconds acquisitions give better results than mFISH. 4.The fact that SKY measures the entire spectrum at each point enables the completely extraction of the emission spectrum of each dye. Errors due to cross talk, high background or wrong contrast of specific layers are minimized. The outcome is therefore a robust classification of chromosomes. 5.Accurate quantification of non specific staining background is subtracted accurately. The acquisition is invariant the intensity variation of dyes. 6.Ability to analyze overlaps and inter-mix chromosomal translocations seen consistently in cancer. 7.The level of automation of a system also influences the accuracy, repeatability and mainly the throughput. SKY is fully automatic, from the acquisition stage right through to the analysis and verification. 8.Interferometer (Optical Head) is manufactured only by ASI and programmed to function with ASI's HiSKY imaging software. Spectral range of interferometer: 400-1000 microns (with special setup even in the UV from 350-400nm). 9.User defined spectral resolution (from 6nm to 50nm or larger gap per channel). ASI's spectral imaging system can be setup to give as low as 3nm FWHM at 400nm (Full Width at Half Maximum of the peak of a narrow signal). 10. This system can also work for non-polarized light. ASI's interferometer imaging system does not block transmitted light. Has a higher resolution per image pixel (16-bit spectral data per pixel). 11.Cooled Digital Camera - works with cooled to -20 degrees Celsius (as well as non-cooled) 12 bit digital camera with 1.3M pixels, ~70% peak quantum efficiency. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the researcher to determine if the party can perform this requirement. An original and one copy of the capability statement must be received in the contracting office on or before 11:00 am ET on September 14, 2009 to Melissa Marino, Contract Specialist. All questions must be in writing and can be faxed to 301-402-4513 or sent via email to marinome@mail.nih.gov. It is the vendor's responsibility to ensure questions have been received. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractors must be registered in and have valid certifications in the Central Contractor Registration (CCR), www.ccr.gov and the Online Representations and Certifications Application (ORCA) www.orca.bpn.gov. Please reference NCI-90121-MM on all correspondence.
 
Web Link
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/NCI-90121-MM/listing.html)
 
Record
SN01934518-W 20090902/090901002430-5ed68bbd3442a248999047acba94b50f (fbodaily.com)
 
Source
FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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