MODIFICATION
B -- Sequencing of Rhesus Monkey Brain Genes
- Notice Date
- 9/2/2009
- Notice Type
- Modification/Amendment
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, Food and Drug Administration, Office of Field Finance & Acquisition Services, HFT-320, 3900 NCTR Road, Jefferson, Arkansas, 72079-9502
- ZIP Code
- 72079-9502
- Solicitation Number
- 09-222-SOL-00113
- Archive Date
- 9/19/2009
- Point of Contact
- Nicholas E Sartain, Phone: 870-543-7370
- E-Mail Address
-
nick.sartain@fda.hhs.gov
(nick.sartain@fda.hhs.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a combined synopsis/solicitation for commercial items prepared in accordance with the Federal Acquisition Regulation (FAR) format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. This requirement is being issued in conjunction with FAR Part 13 Simplified Acquisition Procedures, as applicable. The solicitation number is 09-222-SOL-00113. This solicitation is issued as a Request for Quote (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2005-35. The associated North American Industry Classification System (NAICS) Code is-541711 Research and Development in Biotechnology; Small Business Size Standard is 500 employees. The Food and Drug Administration is soliciting for a firm to capture exons expressed in the brain from genomic DNA of Rhesus monkeys. The captured fragments of DNA will then be sequenced on the NextGen Sequencer. The goal of this project is to identify novel DNA sequence variants in rhesus monkeys, focusing on genes that are expressed in the brain. This will be accomplished by sequencing the exons and 5' flanking sequences of several hundred (-380) genes from rhesus macaques. DNA samples from ten rhesus monkeys will be sequenced for each gene segment (exon or 5' flanking region), and those sequences will be compared across individual macaques to identify single nucleotide polymorphisms and other molecular variations among these ten animals. The contractor will also provide the government 5-10 additional rhesus macaques in order to expand the available information concerning between animal variations. Study Design DNA samples for ten rhesus macaques (Macaca mulatta) will be provided the FDA. The contractor will design a Nimblegen microarray that will capture exons and 5' flanking sequences for the selected rhesus macaque brain genes. The ten FDA macaque samples will be used to make DNA capture libraries, and then five of the samples will be pooled for sequencing. The resulting aliquot of eluted DNA (substantially enriched for the target genes) will be prepared for Roche 454 Titanium DNA sequencing. The first pool of five samples will undergo two runs of Roche 454 Titanium sequencing, resulting in average sequence coverage of approximately 6X of each animal for each gene. The second set of five FDA DNA samples will undergo the same library construction and Nimblegen DNA capture using an equivalent microarray with the same -380 genes. The second five samples will pooled and sequenced in one run of Roche 454 Titanium sequencing, to produce about 3X coverage for each animal. BCM will analyze the resulting sequences and determine SNP and other DNA polymorphisms that distinguish these ten FDA animals. BCM will also provide the equivalent sequence information for 5 additional animals, which will allow further identification and validation of segregating polymorphisms within rhesus macaques for these genes. The contractor will make available to NCTR the full DNA sequence information obtained for the -380 genes (exons and 5'flanking sequences) from the ten DNA samples provided by the FDA and the five additional samples provided by the contractor. The contractor will also provide to FDA a list of DNA sequence variants derived from the combined set of ten FDA animals and 5 contractor provided animals. Contract Type: Commercial Item-Firm Fixed Price. Simplified acquisition procedures will be utilized. FOB Point destination. To be delivered 365 days or less after receipt of order (ARO) FOB Point of Delivery for Services and Supplies will be the FDA/NCTR located at 3900 NCTR Road, Jefferson, AR 72079-9502. Payment terms net 30 days. Standard commercial warranty is required if applicable. The provision at FAR 52.212-1 Instructions to Offerors-Commercial Items applies to this solicitation. The following addenda have been attached to this provision: None The provision at FAR 52.212-2 Evaluation-Commercial Items is applicable to this solicitation. The specific evaluation criteria to be included in paragraph (1) of that provision are as follows: The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be the most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate quotes: (i) Technical capability of the items/services offered to meet the Government's requirement. (ii) Past Performance (iii) Price. Technical and past performance, when combined, are significantly more important when compared to price in determining the best value to the government. Technical acceptability will be determined by review of information submitted by the offeror which must provide a description in sufficient detail to show that the product offered meets the government's requirement. The tradeoff process will be utilized meaning that tradeoffs among price and technical capability will allow the government to accept other than the lowest priced technically acceptable offer. The Government will evaluate the technical capability factor as to whether the offer meets or exceeds the minimum specifications. The contractor must be experienced with genomics research, sequencing technology and computational analysis for genomics. Past performance will be evaluated based on information provided by the offeror as to actual sales of the proposed products to industry or government agencies. Past performance will consider the ability of the items to meet technical specifications as advertised by the offeror, past record of meeting delivery schedules and service/maintenance reputation. The government reserves the right to make an award without discussions. The government is not responsible for locating or securing any information, which is not identified in the proposal. To ensure information is available, vendors must furnish as part of their proposal all descriptive material necessary for the government to determine whether the product and/or service meets the technical requirements. The offer shall include technical specifications, descriptive material, literature, brochures and other information corresponding to each minimum required item, which demonstrates the capabilities of the offeror. The Government reserves the right to obtain information for use in the evaluation from any and all sources including sources outside of the Government. The Provision at FAR 52.212-3, Offeror Representations and Certifications-Commercial Items, applies to this acquisition. An offeror should complete only paragraph (b) of this provision if the offeror has completed the annual representations and certifications electronically at http://orca.bpn.gov. The clause at 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. The following addenda have been attached to the clause. The supplies and/or services delivered hereunder shall be inspected and accepted at destination by the contracting officer's representative (COR) specified at award. If the supplies or services are acceptable, the COR shall promptly forward a report of inspection and acceptance to the paying office. If the supplies or services are not acceptable, the COR shall document the nonconforming items/services and immediately notify the contracting officer. The clause at FAR 52.212-5, Contract Terms and Conditions Required to Implement Statues or Executive Orders-Commercial Items applies to this acquisition. The following additional FAR clauses cited in this clause are applicable: 52.222-3, 52.222-19, 52.222-21, 52.222-26, 52.222-36, 52.225-1, 52.225-13, and 52.232-33. The following additional provisions and/or clauses apply: 52.204-7. Clauses and provisions are incorporated by reference and apply to this acquisition. Clauses and provisions can be obtained at http://www.arnet.gov/far/ CCR Requirement - Company must be registered in the Central Contractor Registration (CCR) system before an award could be made to them. If company is not registered in CCR, they may do so by going to CCR web site at http://www.ccr.gov. The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable to this solicitation notice. It is the offeror's responsibility to monitor the internet site for the release of an amendment to the combined synopsis/solicitation (if any). Offerors that fail to complete the required representations and certifications, or reject the terms and conditions of the solicitation, may be excluded from consideration. All responsible sources may submit an offer, which if timely received, shall be considered. The offer must reference solicitation number 09-222-SOL-00113. The offers are due in person, by postal mail, fax or email to the point of contact listed below on or before September 4, 2009 by 13:00 hours (Central Standard Time in Jefferson, Arkansas) at the Food and Drug Administration, OC/OSS/OFFAS, Attn: Nick Sartain, 3900 NCTR Road, HFT-320, Jefferson, AR 72079-9502. For information regarding this solicitation, please contact Nick Sartain at (870) 543-7370, FAX (870) 543-7990 or email nick.sartain@fda.hhs.gov.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/FDA/NCTR/09-222-SOL-00113/listing.html)
- Place of Performance
- Address: 3900 NCTR Road, Jefferson, Arkansas, 72079, United States
- Zip Code: 72079
- Zip Code: 72079
- Record
- SN01937923-W 20090904/090903001128-caf91df81e91d664389c873f6c33acfd (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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