AWARD
66 -- TaqMan Human microRNA Array A&B
- Notice Date
- 3/25/2010
- Notice Type
- Award Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- RFQ-NCI-90180-MM
- Archive Date
- 4/9/2010
- Point of Contact
- Melissa P Marino, Phone: 301-402-4509, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
marinome@mail.nih.gov, cr214i@nih.gov
(marinome@mail.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Award Number
- HHSN261201000194P
- Award Date
- 3/25/2010
- Awardee
- Applied Biosystems LLC DUNS# 001184480, 850 Lincoln Centre Drive, Foster City, California 94404-1128, United States
- Award Amount
- $109,800.00
- Description
- Contractor Requirements: The contractor shall provide eighty Applied Biosystems Taqman MicroRNA Arrays. Unique features of the Applied Biosystems TaqMan MicroRNA Arrays include: 1.)Reaction vessel uses microfluidic technology in a 384-well plate format. 2.)The reaction vessel has 8 sample loading ports and must use centrifugation to distribute the samples into each of the 48 wells serviced by each port. 3.)The reaction vessel is compatible with the Applied Biosystems 7900HT Sequence Detection System (a real-time PCR instrument). NCI already has this system in the lab, so there is no need to purchase additional equipment. 4.)The reaction vessel is pre-loaded with pre-designed 5'-nuclease assays using TaqMan® probes. a. The assay contains the necessary primers and probes to perform both reverse transcription of the targeted miRNA, and real time PCR detection of that miRNA transcript only. b. The assay id specific for the mature form of the miRNA transcript. c.The real time PCR detection method includes TaqMan® probes (5' nuclease chemistry) with MGB quenching the 3' end of the probe. d.The TaqMan® probe is cleaved when the probe hybridizes to the target. e.The assay has a minimum dynamic range of 6 logs. 5.)The vendor shall supply all the necessary consumables to perform real-time quantitative PCR, including PCR reagents designed for use with the fluorogenic 5' nuclease assay. All PCR reagents contain a passive internal reference dye to minimize well-to-well variability. 6.)The vendor shall provide comprehensive assay design and development guidelines for real-time quantitative PCR. 7.)The vendor shall offer Telephone Technical Support and Field Applications/Sales/Service Support to help solve chemistry and instrumentation problems encountered with real-time quantitative PCR. 8.)High specificity: single base pair discrimination of mature miRNA. 9.)Sensitivity: single copy of target 10.)Linear Dynamic Range: 7-9 logs of linear dynamic range 11.)Ease of use: Easily implementable on existing 7900HT, with only 2 steps that take approximately 3-10 minutes total hands on time average per sample when operating in high throughput (15-30 minutes per sample hands on time in low throughput) 12.)Low sample input: Ability to assess single cells or low cell population (Non-biased assessment of single cells with the TaqMan Array). Ability to do Laser Capture Microdissection (LCM), FFPE (Formalin Fixed Paraffin Embedded) tissues, and every other tissue with minimally 1 cell and no limitation on abundant sample. 13.)Highly parallel miRNA profiling/detection in the TaqMan Array Cards (TLDA) with TaqMan miRNA Gold Standard Assays These Arrays must be compatible with the 7900HT Real-Time PCR machine and Sorvall Legend RT+ centrifuge. Brand name justification: Use of a different platform would result in a decrease of sensitivity and specificity. Further, alternate platforms would require procurement of additional, costly equipment and re-training of staff.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/Awards/HHSN261201000194P.html)
- Record
- SN02102659-W 20100327/100325235050-4bac5528deb6ee151c375daf93cf50ed (fbodaily.com)
- Source
-
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