PRESOL | 68 | The US Fish & Wildlife Service is seeking a contractor to synthesize approximately 2,500 g of bisazir to be used as a chemosterilant to control male sea lampreys in the Great Lakes.

FBO DAILY ISSUE OF JULY 30, 2010 FBO #3170
SOLICITATION NOTICE

68 -- The US Fish & Wildlife Service is seeking a contractor to synthesize approximately 2,500 g of bisazir to be used as a chemosterilant to control male sea lampreys in the Great Lakes.

Notice Date: 7/28/2010
Notice Type: Combined Synopsis/Solicitation
Contracting Office: Construction and Acquisition Division, Region 3 U.S. Fish and Wildlife Service1 Federal Drive, Room 695 Fort Snelling MN 55111
ZIP Code: 55111
Solicitation Number: 30181AQ400
Response Due: 8/13/2010
Archive Date: 7/28/2011
Point of Contact: Jeff Worthley Contract Specialist 6127135188 jeff_worthley@fws.gov;
E-Mail Address: Point of Contact above, or if none listed, contact the IDEAS EC HELP DESK for assistance
(EC_helpdesk@NBC.GOV)
Small Business Set-Aside: N/A
Description: This is a combined synopsis/solicitation for gravel prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. Proposals will be evaluated on the basis of total cost (all or nothing). Please include shipping charges in your price. MATERIAL REQUIREMENTS: Line 1 Synthesize 2,440 grams of bisazir (P,P-bis[1-aziridinyl]-N-methylphosphinothioic amid) Weight of bisazir will be exact to a tenth of a gram. 5 containers each to contain 1.0 g, for a total of 5 g3 containers each to contain 5.0 g, for a total of 15g4 containers each to contain 10.0 g, for a total of 40 g122 containers each to contain 20.0 g, for a total of 2,440 gIndividual containers will be sealed shut and packaged in secondary containment (i.e. sealed plastic bag). The supplier will provide a chemical analysis of the material. The product must have a minimum melting point of 70 C. A purity of >95% will be shown by gas chromatographic analysis (flame ionization detector). A copy of the chromatogram will be provided with the product. The product will be packaged in individual containers, each size different in color or shape, and with proper labeling. The supplier will provide a material data safety sheet. The product will be shipped according to all federal and state regulations and the shipping packaging will be clearly marked for easy identification by receiving personnel. The U.S. Fish and Wildlife Service will verify purity before payment is made. SOLICITATION / QUOTE TIMING & REQUIREMENTS: Quotes are due on or before Friday, August 13, 2010. Please fax or e-mail bids to Contract Specialist Jeff Worthley at fax number 612-713-5151 or jeffrey_worthley@fws.gov. Please call Jeff with any questions at 612-713-5188. Be sure to note whether you have an up to date ORCA certification (https://orca.bpn.gov/). If you do not have a current ORCA certification please include a completed 52.212-3 Offeror Representations and Certifications (http://farsite.hill.af.mil/vffara.htm). For technical questions please contact Michael Twohey 906-226-1226. DELIVERY LOCATION:Michael Twohey U.S. Geological SurveyBiological Resources DivisionHammond Bay Biological Station11188 Ray RoadMillersburg, MI 49759 The product must be delivered by February 1, 2011 Procedures for the Synthesis of Bisazir from the U.S. Department of Agriculture, Insect Chemosterilants Laboratory in Beltsville, Maryland Bis(aziridinyl) methylaminophosphine sulfide was prepared according to the following reactions: 2CH3NH2 + PSCl3 ether'0-5 CH3NHPSCl2 + CH3NH2-HCl CH3NHPSCl2 + 2HN + 2Et3N benzene'0-5 CH3NHPS(N )2 (Bisazir) + 2Et3NHCL Methylaminothiophosphoryl chloride was obtained by careful addition of 275 g (8.86 mole) of methylamine to 750 g (4.4 mole) of thiophosphoryl chloride in 3 L of anhydrous ether. The amine had been collected by cooling in a flask and was allowed to distill into the reaction mixture through a tube that extended under the liquid. The reaction mixture was vigorously stirred and temperature maintained below 5 C with a salt-ice bath. After the addition had been completed, the mixture was washed three times with 1 L portions of water and once with 500 ml of a saturated sodium chloride solution. The ether was removed under vacuum and the residue was held at 50 C under 20 mm pressure to constant weight. Yield 675 g (4.1 moles). Bis(aziridinyl)methylaminophosphine sulfide was prepared by adding the 675 g (4.1 moles) of dichloride to a solution of 390 g (9.0 moles) of ethylamine and 850 g (8.5 moles) of triethylamine in 5 L of benzene. The reaction mixture was vigorously stirred and cooled with a salt-ice bath. The temperature was held below 8 C during the addition and then raised to 35 C for 1 hr. The mixture was washed twice with 2 L portions of water and once with 500 ml of sodium chloride solution. The benzene was stripped under vacuum and the solid residue recrystallized once from 2 L of cyclohexane and once from 1 L of 95% ethanol. Yield 350 - 450 g (melting point 70-72.5 C). Thiosulfate titration 99% of theoretical. STANDARD LABORATORY OPERATING PROCEDURES FOR CONDUCTING ANALYSIS Procedure Title: Determination of the concentration of bisazir in water using Waters Breeze HPLC. Area of Applicability: This procedure is applicable to chemistry laboratory staff at the Hammond Bay Biological Station. Scope: To describe procedures for the determination of bisazir (P,P-bis(1-azirdinyl)-N-methylphosphinothioic amide) in water and waste water samples. Principle: To use a standard method for measurement of bisazir in water and waste water samples. Equipment: Waters Breeze High Performance Liquid Chromatographic System:1. Waters 717 WISP Auto injector2. Waters 1525 Binary Pump3. Waters 2487 Dual Absorbance Detector4. Waters Breeze software Eppendorf pipettes ranging in size from 10 uL to 100 uL Solid phase columns:1. Phenomenex Luna, 10 micron, C18(2) reverse phase column (4.5 x 250 mm) 2. Phenomenex Strata-X 33micron, polymeric sorbent 60mg/3mL (part #8B-S100-UBJ) Procedure: General:1. Bisazir is a suspected mutagenic and carcinogenic compound. Proper safety clothing and equipment will be used when analyzing any samples for bisazir. Follow HBBS SOP 339.0a. cotton scrub suitsb. gloves--latex exam gloves worn under a pair of natural rubber latex gloves of 18 mil or greater thicknessc. Tyvek coveralld. Tyvek head cover with capee. Tyvek shoe coversf. Racal Breathe Easy Turbo Unit forced air respirator Reagents used:1. Methanol (MEOH), reagent HPLC grade2. Water, reagent HPLC grade3. Bisazir, Chemical Abstracts Services #13687-09-074. Ethanol, (EtOH) 5. Bisazir Standard Solution (100 mg/L active ingredient)a. In a 100 mL volumetric flask: Dissolve 10.0 mg (active ingredient) bisazir in approximately 5 mL EtOH.b. Dilute up to 100mL using HPLC grade water.c. If refrigerated, the standard has a shelf life of about 30 days.6. Working Standardsa. Prepare new working standards weekly and keep refrigerated.b. Use filtered lake water for dilution if testing effluents. Use HPLC water for dilution if preparing and working with lab samples only. c. 25 ug/L--dilute 25 uL of 100 mg/L bisazir to 100 mL d. 50 ug/L--dilute 50 uL of 100 mg/L bisazir to 100 mL e. 75 ug/L--dilute 75 uL of 100 mg/L bisazir to 100 mL f. 100 ug/L-dilute 100 uL of 100 mg/L bisazir to 100mL Sample preparation:1.If samples are extremely dirty they may be filtered by vacuum filtration using a flask and glass fiber filter. 2.SPE conditioning:a. Place SPE on the a vacuum manifold. b. Pass 5 mL of MEOH through the SPE. The flow rate should be about 10 mL/min or less; if greater, remove more caps from the manifold so less suction is applied to the SPE. Note: Do not allow SPE to dry before passing a sample through it.c. Rinse the column with at least 10 mL of HPLC water. 3.Sample extraction:a. Be sure the 1000 mL water trap is empty and add 0.5 mL conc. H2SO4 to the trap to destroy any residual bisazir. b. Label the SPE columns (conc. of standard or sample identifier). c. Place 75 mL reservoir on SPE. d. Pass 100 mL sample or standard through the labeled and conditioned SPE; flow rate should be 10 mL/min or less. e. Using a wash bottle, rinse the 100-mL graduated cylinder two times using filtered lake water. f. Using a wash bottle, rinse the reservoir with filtered lake water. g. Remove empty 75 mL reservoir from SPE. h. Allow SPE to dry on the vacuum manifold for an additional 3 minutes (SPEs may be refrigerated and eluted at a later time). 4.Sample recovery:a. Using the proper sized holding rack, place HPLC vials directly under SPEs to be extracted. b. Using an Eppendorf pipette for the final extraction, pass 2.5mL methanol through each SPEc. Cap vials as soon as possible to prevent evaporation. 5.HPLC analysis:a.Always follow manufacturer's instructions and station's instrument operating procedures. b.Be sure HPLC waste container is empty and add 1 mL concentrated H2SO4 to destroy bisazir remaining after elution through column. c.HPLC systems, columns, and sample matrix may be slightly different and the following procedures may have to be modified slightly.d.Operating conditions:1) Stationary phase-4.6 x 250 mm Phenomenex LUNA column 2) Mobile phase-gradient:a) 2.5 mL/minb) 85% water/15% methanol 0-25mingradient to 100% methanol 25-30min100% methanol 30-35minGradient to 85/15 35-40minc) Use on-line degassing system or helium sparge3) Injection volume--50 ?L. 4) Detector wavelength--210 nm. 5) Retention time--about 21 min. 6. Record data in HPLC Loga. Record date, procedure, mobile phase, column identification, injection volume, detector wavelength, concentration of standards, peak area, and retention time. Calculations:1. The Waters Breeze software will determine retention times, peak area, correlation, slope, intercept and concentration. CLAUSES::52.212-01 Instructions to Offerors--Commercial Items 52.212-3 Offeror Representations and Certifications -- Commercial Items.52.212-04 Contract Terms and Conditions--Commercial Items 52.212-05 CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS--COMMERCIAL ITEMS X (8)(i) 52.219-06, Notice of Total Small Business Set-Aside (June 2003) (15 U.S.C. 644). X (18) 52.219-28, Post Award Small Business Program Rerepresentation (APR 2009) (15 U.S.C. 632(a)(2)). X (19) 52.222-03, Convict Labor (June 2003) (E.O. 11755). X (20) 52.222-19, Child Labor-Cooperation with Authorities and Remedies (AUG 2009) (E.O. 13126). X (21) 52.222-21, Prohibition of Segregated Facilities (Feb 1999). X (22) 52.222-26, Equal Opportunity (MAR 2007) (E.O. 11246). X_ (24) 52.222-36, Affirmative Action for Workers with Disabilities (Jun 1998) (29 U.S.C. 793). X_ (31)(i)52.225-03, Buy American Act-Free Trade Agreements-Israeli Trade Act (JUNE 2009) (41 U.S.C. 10a-10d, 19 U.S.C. 3301 note, 19 U.S.C. 2112 note, 19 U.S.C. 3805 note, Pub. L. 108-77, 108-78, 108-286, 108-302, 109-53, 109-169, 109-283, and 110-138. X (33) 52.225-13, Restrictions on Certain Foreign Purchases (JUNE 2008) (E.O's, proclamations, and statutes administered by the Office of Foreign Assets Control of the Department of the Treasury). X (38) 52.232-33, Payment by Electronic Funds Transfer-Central Contractor Registration (Oct 2003) (31 U.S.C. 3332). (i) 52.203-13, Contractor Code of Business Ethics and Conduct (DEC 2008) (Pub. L. 110-252, Title VI, Chapter 1 (41 U.S.C. 251 note)). 52.211-16 VARIATION IN QUANTITY(a) A variation in the quantity of any item called for by this contract will not be accepted unless the variation has been caused by conditions of loading, shipping, or packing, or allowances in manufacturing processes, and then only to the extent, if any, specified in paragraph (b) below. GOVERNMENT LIAISON - SERVICE DIRECTION (a) The performance required herein shall be subject to the service direction of the Government Liaison (GL) as identified below. As used herein, "service direction" is defined as direction to the contractor that fills in details, suggests possible lines of approach, or otherwise supplements the scope of the work set forth herein and shall not constitute a new assignment, and does not supersede or modify any article or clause of this contract. Additionally the Government Liaison may certify invoices on behalf of the Contracting Officer. (b) The Government Liaison is not authorized to perform, formally or informally, any of the following actions:(1) Promise, award, agree to award, or execute any contract, contract modification, or notice of intent that changes or may change this contact;(2) Waive or agree to modification of the delivery schedule;(3) Make any final decision on any contract matter subject to the Disputes Clause;(4) Terminate, for any reason, the contractor's right to proceed;(5) Obligate in any way, the payment of money by the Government. Only a warranted Contracting Officer is authorized to obligate funds on this or any other contract action. (c) The contractor shall immediately notify the Contracting Officer in writing if the Government Liaison has taken an action (or fails to take action) or issues direction (written or oral) that the contractor considers exceeding the above limitations. (d) The Government Liaison assigned for this contract is:Names: Michael Twohey Telephone Number: 906-226-1226 USGS Biological Resouce DivisionHammond Bay Biological Station1118 Ray RoadMillersburg, MI 49759 (e) Only the Contracting Officer may designate a different Government Liaison. 52.212-02 Evaluation - Commercial Items (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: U.S. Fish and Wildlife Service will verify purity is greater than 95% before payment is made. Technical and past performance, when combined, are ________________________. (Contracting Officer state, in accordance with FAR 15.304, the relative importance of all other evaluation factors, when combined, when compared to price.) (b) Options. The Government will evaluate offers for award purposes by adding the total price for all options to the total price for the basic requirement. The Government may determine that an offer is unacceptable if the option prices are significantly unbalanced. Evaluation of options shall not obligate the Government to exercise the option(s). (c) A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer's specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award.
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Place of Performance: Address: U.S. Geological SurveyBiological Resouces DivisionHammond Bay Biological Station11188 Ray RoadMillersburg, MI 49759; Zip Code: Local Contact: Michael Twohey 906-226-1226
Record: SN02220997-W 20100730/100728235657-2b2d28fadd82e961e2c29b350aa755f7 (fbodaily.com)
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68 -- The US Fish & Wildlife Service is seeking a contractor to synthesize approximately 2,500 g of bisazir to be used as a chemosterilant to control male sea lampreys in the Great Lakes.