SOLICITATION NOTICE
65 -- Roche Sequencing kits
- Notice Date
- 11/8/2010
- Notice Type
- Justification and Approval (J&A)
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Agriculture, Agricultural Research Service, Northern Plains Area, Roman L. Hruska U.S. Meat Animal Research Center, Bldg. 1, State Spur 18D, P.O. Box 166, Clay Center, Nebraska, 68933
- ZIP Code
- 68933
- Solicitation Number
- AG6538S11AA06
- Point of Contact
- Donita J. Furman, Phone: 402-762-4145
- E-Mail Address
-
donita.furman@ars.usda.gov
(donita.furman@ars.usda.gov)
- Small Business Set-Aside
- N/A
- Award Number
- AG6538P11AA06
- Award Date
- 11/8/2010
- Description
- The USDA, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska, intends to enter into a single source contract with Roche Diagnostics, Indianapolis, Indiana, for reagents. The set of reagents to be purchased are sufficient to obtain a full genome coverage of a bovid genome and are sold as a "de novo genome" package including: 7 GS Titanium Sequencing Kit XLR70, part number 05 233 526 001 7 GS Titanium PicoTiter Plate Kit 70x75, part number 05 233 682 001 7 GS FLX Titanium LV emPCR Kit Lib-L, part number 05 618 428 001 1 GS Titanium emPCR Breaking Kits LV/MV, part number 05 233 658 001 1 GS Rapid Library Prep Kit, part number 05 608 228 001 1 GS Titanium Paired End Adapter Kit, part number 05 463 343 001 The reagents and supplies are intended to support running a Genome Sequencer FLX system housed at the U.S. Meat Animal Research Center. The Genome Sequencer FLX instrument (GS FLX) is a DNA sequencer designed and built by 454 Life Sciences and distributed by Roche Applied Science. The technology for this system is based on enrichment of fragmented DNA followed by clonal amplification and pyrosequencing. The sequencer can generate up to 500 million bases per 7.5 hour run, achieve reads averaging 350 to 450 bases, attain over 1,000,000 reads per run, generate single-read accuracy that is greater than 99%, and achieve consensus accuracy greater than 99.99%. Reagents required to run this system include GS Titanium compatible library preparation kits for linker modified single stranded DNA, emulsion based PCR kits for preparative reactions of bead-linked DNA fragments for sequencing, Titanium chemistry sequencing kits, and PicoTiter Plate Kits which provide the surface for sequencing of the DNA fragments. Roche is the sole supplier and distributor of the GS FLX instrument. The GS titanium library preparation reagents, emulsion based PCR kits, sequencing kits, and PicoTiter Plate Kits are only available through Roche. The GS FLX warranty is conditional that customers agree to use the instrument in strict accordance with Roche operating instructions, including Roche sole source specifications regarding reagent kits. To our knowledge, the FLX sequencer makes use of very specialized reagents only available from Roche at the present time in a form suitable for use on the instrument.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USDA/ARS/NPAMARC/AG6538S11AA06/listing.html)
- Place of Performance
- Address: State Spur 18D Bldg 1, Clay Cener, Nebraska, 68933, United States
- Zip Code: 68933
- Zip Code: 68933
- Record
- SN02324084-W 20101110/101108234039-4a2de806636e6603296f58f067f97f03 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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