SOURCES SOUGHT
66 -- Monolith NT.115 Instrument/Microscale Thermophoresis
- Notice Date
- 2/4/2011
- Notice Type
- Sources Sought
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HG)-2011-093-DLM
- Archive Date
- 2/23/2011
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This Sources Sought Notice (SS) is for information and planning purposes only and shall not be construed as a solicitation or as an obligation on the part of the National Heart, Lung, and Blood Institute (NHLBI). The purpose of this SS is to identify qualified small business concerns [including Small Disadvantaged Businesses (SDB), Woman-owned Small Businesses (WOSB), Historically Underutilized Business Zone (HUBZone) Small Businesses, Veteran-Owned Small Businesses (VOSB) and Service-Disabled Veteran-owned Small Businesses (SDVOSB] that are interested in and capable of providing the below Engineering and Software Support Services for a Laboratory Workflow System. The NHLBI does not intend to award a contract on the basis of responses nor otherwise pay for the preparation of any information submitted. As a result of this Sources Sought notice, the NHLBI may issue a Request for Quote (RFQ). THERE IS NO SOLICITATION AVAILABLE AT THIS TIME. However, should such a requirement materialize, no basis for claims against NHLBI shall arise as a result of a response to this Sources Sought notice or the NHLBI’s use of such information as either part of our evaluation process or in developing specifications for any subsequent requirement. The NHLBI is seeking capability statements from all eligible small business concerns [particularly Small Disadvantaged Businesses (SDB), Woman-owned Small Businesses (WOSB), Historically Underutilized Business Zone (HUBZone) Small Businesses, Veteran-Owned Small Businesses (VOSB) and Service-Disabled Veteran-owned Small Businesses (SDVOSB)] under the North American Industry Classification System (NAICS) code 333314, Optical Instrument and Lens Manufacturing with a business size standard of 500. Based on the responses received from this SOURCES SOUGHT announcement, the proposed acquisition may be solicited as a Total Small Business Set-Aside. All eligible small business concerns responding to this Sources Sought Announcement must have the capabilities to provide the below services within 30 days after receipt of purchase order. Interested parties are expected to review this notice to familiarize yourself with the requirements of this project; failure to do so will be at your firm’s own risk. Background: The Assay Development and Screening Technology (ADST) Laboratory, part of the NIH Center for Translational Therapeutics (NCTT), is requesting a NanoTemper Monolith instrument. This instrument is needed to handle the existing protocols of cell-based assays established by the NIH Chemical Genomics Center (NCGC), a branch of the NCTT. The implementation of existing technology and protocols will ensure consistency and accuracy between all laboratories under the NCTT. The ADST Laboratory is working to develop novel assays as well as to apply existing assays to a spectrum of rare and neglected diseases under the Therapeutics for Rare and Neglected Disease (TRND) initiative. This development of target specific cell based assays for rare and neglected diseases must also remain compatible with the automated robotic qHTS facility at the NCGC (NIH Chemical Genomic Center) to insure the efficient and accurate transfer of assays between laboratories. Description/Specifications: NanoTemper.Monolith NT.115 Instrument Microscale Thermophoresis (MST) enable real-time, immobilization free analysis of biomolecules providing information on the affinity, stoichiometry, enzyme kinetics, and aggregation properties of biomolecules in buffers and complex biological liquids including blood serum and cell lysate. Equipped with the standard sample tray, it can process automatically up to 16 samples per run., 3 models of the Monolith NT.115 instrument are offered, which differ in the excitation/detection spectrum to detect blue, green and/or red fluorescent dyes. Microscale Thermophoresis can monitor the binding of single ions (40Da) or small molecules (300Da) to a target as well as the binding of ribosomes (2.5MDa). This instrument is also easy to handle and allows to measure the binding of biomolecules as well as the activity of enzymes. Microscale Thermophoresis monitors binding and biochemical activity of biomolecules under close-to-native conditions: • immobilization-free; • In a solution of choice, ranging from standard and proprietary buffers to complex bioliquids including blood serum or cell lysates; and, • at a temperature of choice. The capillary format is easy to handle and offers maximum flexibility in the experiment scale. The sample tray format allows to process automatically up to 16 capillaries (e.g. for a detailed KD-analysis), or alternatively to perform smaller pilot experiments or end point measurements involving 2-3 capillaries only. Microscale Thermophoresis requires approximately 100fold less sample material compared to standard technologies. Capillary volume: 3-5 µl. 1-100 nM of the labeled compound. The Monolith NT.115 instrument is supported by a software for data acquisition and analysis. Microscale Thermophoresis (MST) technology uses a fluorescence microscope equipped with a dichroic mirror that couples a IR-Laser into the path of fluorescence light. The heating laser is focused with the same objective used for fluorescence detection. This enables a precise local heating (1-6 K) of the glass capillary and simultaneously to observe and measure local changes of fluorescence intensity due to the motion of labeled molecules in the glass capillaries. Fluorescently labeled molecules or particles are initially distributed evenly and diffuse freely in solution. By switching on the IR-Laser, the molecules experience a thermophoretic force in the temperature gradient and typically move out of the heated spot. In the steady state, this molecule flow is counterbalanced by ordinary mass diffusion. After turning off the laser, the particles diffuse back towards a homogeneous distribution. The following stages are recorded for each sample: fluorescence signal before turning the IR laser on, fast temperature-dependent changes in fluorescence intensity of the analyzed molecules, thermophoresis and back diffusion after switching the laser off. Interested parties should submit a tailored capability statement for this requirement, not to exceed 20 single-sided pages (including all attachments, resumes, charts, etc.) presented in single-space and using a 12-point font size minimum, that clearly details the ability to perform the aspects of the notice described above and in the attached draft statement of work. All proprietary information should be marked as such. Statements should also include an indication of current certified small business status; this indication should be clearly marked on the first page of your capability statement (preferably placed under the eligible small business concern’s name and address). Responses will be reviewed only by NIH personnel and will be held in a confidential manner. All capability Statements sent in response to this SOURCES SOUGHT notice must be submitted electronically (via email) to Dorothy Maxwell, Contract Specialist, at maxwelld@mail.nih.gov in either MS Word or Adobe Portable Document Format (PDF), by February 8, 2011, 12:00 PM, EST. All responses must be received by the specified due date and time in order to be considered.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HG)-2011-093-DLM/listing.html)
- Place of Performance
- Address: NIH, Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN02373623-W 20110206/110204234349-e808d3ed84f909d610b6c1ed7acf2b29 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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