SOLICITATION NOTICE
66 -- Monolith NT.115 Instrument/ Microscale Thermophoresis (MST)
- Notice Date
- 2/17/2011
- Notice Type
- Presolicitation
- NAICS
- 333314
— Optical Instrument and Lens Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NHLBI-CSB-(HG)-2011-104-DLM
- Archive Date
- 3/9/2011
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) intends to negotiate and award a purchase order on a noncompetitive sole source basis to NanoTemper Technologies, Amalienstr.54, Munchen Bayern 80799, DEU to procure a Monolith NT.115 Instrument/ Microscale Thermophoresis (MST). The sole source determination is based on the fact that National Institute of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency conducting and supporting making medical discoveries that improve people’s health and save lives. The mission of the NIH Chemical Genomics Center (NCGC) is to apply the tools of small molecule screening and discovery to the development of chemical probe research tools, for use in the study of protein and cell functions, and biological processes relevant to physiology and disease. The Assay Development and Screening Technology (ADST) Laboratory, part of the NIH Center for Translational Therapeutics (NCTT), is requesting a NanoTemper Monolith instrument. This instrument is needed to handle the existing protocols of cell-based assays established by the NIH Chemical Genomics Center (NCGC), a branch of the NCTT. The implementation of existing technology and protocols will ensure consistency and accuracy between all laboratories under the NCTT. The ADST Laboratory is working to develop novel assays as well as to apply existing assays to a spectrum of rare and neglected diseases under the Therapeutics for Rare and Neglected Disease (TRND) initiative. This development of target specific cell based assays for rare and neglected diseases must also remain compatible with the automated robotic qHTS facility at the NCGC (NIH Chemical Genomic Center) to insure the efficient and accurate transfer of assays between laboratories. NanoTemper.Monolith NT.115 Instrument Microscale Thermophoresis (MST) enable real-time, immobilization free analysis of biomolecules providing information on the affinity, stoichiometry, enzyme kinetics, and aggregation properties of biomolecules in buffers and complex biological liquids including blood serum and cell lysate. Equipped with the standard sample tray, it can process automatically up to 16 samples per run., 3 models of the Monolith NT.115 instrument are offered, which differ in the excitation/detection spectrum to detect blue, green and/or red fluorescent dyes. Microscale Thermophoresis can monitor the binding of single ions (40Da) or small molecules (300Da) to a target as well as the binding of ribosomes (2.5MDa). This instrument is also easy to handle and allows to measure the binding of biomolecules as well as the activity of enzymes. Microscale Thermophoresis monitors binding and biochemical activity of biomolecules under close-to-native conditions: • immobilization-free; • In a solution of choice, ranging from standard and proprietary buffers to complex bioliquids including blood serum or cell lysates; and, • at a temperature of choice. The capillary format is easy to handle and offers maximum flexibility in the experiment scale. The sample tray format allows to process automatically up to 16 capillaries (e.g. for a detailed KD-analysis), or alternatively to perform smaller pilot experiments or end point measurements involving 2-3 capillaries only. Microscale Thermophoresis requires approximately 100fold less sample material compared to standard technologies. Capillary volume: 3-5 µl. 1-100 nM of the labeled compound. The Monolith NT.115 instrument is supported by a software for data acquisition and analysis. Microscale Thermophoresis (MST) technology uses a fluorescence microscope equipped with a dichroic mirror that couples a IR-Laser into the path of fluorescence light. The heating laser is focused with the same objective used for fluorescence detection. This enables a precise local heating (1-6 K) of the glass capillary and simultaneously to observe and measure local changes of fluorescence intensity due to the motion of labeled molecules in the glass capillaries. Fluorescently labeled molecules or particles are initially distributed evenly and diffuse freely in solution. By switching on the IR-Laser, the molecules experience a thermophoretic force in the temperature gradient and typically move out of the heated spot. In the steady state, this molecule flow is counterbalanced by ordinary mass diffusion. After turning off the laser, the particles diffuse back towards a homogeneous distribution. The following stages are recorded for each sample: fluorescence signal before turning the IR laser on, fast temperature-dependent changes in fluorescence intensity of the analyzed molecules, thermophoresis and back diffusion after switching the laser off. Industry Classification (NAICS) Code is 333314, Optical Instrument and Lens Manufacturing, with size standard of 500 employees. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001).and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-48 (January 31, 2011). This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this synopsis, by February 22, 2011, 7:30 a.m. Eastern Standard Time. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Inquires to this announcement, referencing synopsis number NHLBI-CSB-(HG)-2011-104-DLM, may be submitted to the National Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI-CSB-(HG)-2011-104-DLM/listing.html)
- Place of Performance
- Address: NIH/NHGRI, Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN02382926-W 20110219/110217234759-1fe50690d90e845f4a2350b8922f2668 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
| FSG Index | This Issue's Index | Today's FBO Daily Index Page |