SOLICITATION NOTICE
66 -- Quantitative Real Time PCR Machine - SF-18
- Notice Date
- 7/13/2011
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Agriculture, Agricultural Research Service, Pacific West Area, Maricopa, AZ, 21881 North Cardon Lane, Maricopa, Arizona, 85239, United States
- ZIP Code
- 85239
- Solicitation Number
- AG81J8S11AA20
- Archive Date
- 8/3/2011
- Point of Contact
- Sheri Marcelo, Phone: 520-316-6314, Fred Gifford, Phone: 510-559-6017
- E-Mail Address
-
sheri.marcelo@ars.usda.gov, Frederick.Gifford@ars.usda.gov
(sheri.marcelo@ars.usda.gov, Frederick.Gifford@ars.usda.gov)
- Small Business Set-Aside
- N/A
- Description
- SF-18 The U.S. Department of Agriculture, Agricultural Research Service, Pest Management and Biocontrol and Plant Physiology Research units in Maricopa, AZ has a requirement for a has a requirement for a quantitative real time PCR machine. The Pest Management and Biocontrol Research Unit mission is to develop unique and improved biological, behavioral, cultural, and genetic based methods to reduce losses by insects and mites affecting crops in arid land production areas of the U.S. The goals are economically, socially and environmentally acceptable pest population suppression technologies based on the use of behavioral chemicals, genetics, cultural practices, biological control, sampling and decision aids, modeling and population dynamics of pests and their natural enemies. Emphasis is placed on development of fully integrated, ecologically-based management strategies in a changing agricultural environment that includes recognition of the agriculture-urban interface. The mission of the Plant Physiology and Genetic Research Unit is to increase the productivity, profitability and sustainability of agriculture in arid environments by developing new germplasm for both traditional and alternative crops and by improving plant responses to abiotic stresses and to changes in the global environment. Duns Number: Contractor must be registered in CCR Shipping Cost: FOB Destination Preferred Method; however if you choose to quote FOB Origin, please provide estimated shipping cost. Delivery Schedule: Please provide your estimated delivery date with your quotation Evaluation: Quotations received in response to this synopsis/solicitation shall be evaluated on best value to the government. The following FAR Clauses apply to this solicitation. Offerors may obtain full text versions of these clauses electronically at www.arnet.gov.far FAR 52.212-4 Contract Terms and Conditions-Commercial Items (June 2010) FAR 52.212-5, Contract Terms & Conditions Required to Implement Statutes or Executive Orders-Commercial Items (May 2011) FAR 52.204-7 Central Contractor Registration (April 2008) FAR 52.223-15 Energy Efficiency in Energy-Consuming Products (December 2007) All quotes must be submitted by Tuesday, July 19, 2011 by 9:00 am Mountain Standard Time by fax (520) 316-6329 or email sheri.marcelo@ars.usda.gov Attachment - SF-18 Brand Name or Equal - Specifications - Bio-Rad CFX96 Real Time PCR Detection System •· Dynamic gradient thermocycler - single reduced-mass C1000 sample block with six independently controlled thermal electric units resulting in temperature accuracy of +/-0.2 degC and temperature uniformity of +/-0.25 degC within 10 seconds after clock start. •· Automatic calculation of temperature differences and produce gradient with eight different temperature zones (i.e. rows) ranging from 1-24 degC. •· Open platform for reagents and consumables, including strip tubes, 96-well plates, enzymes, and fluorophores. •· Reaction volumes of 1-50 microliters. •· Multiplex capable of up to five dyes in single well using six excitation LEDs specifically matched with five different filtered photodiodes for specific fluorophore detection and a sixth LED matched for FRET applications. Filter sets over 6 channels include detection of FAM and SYBR Green in channel 1, VIC, HEX, TET, Cal Gold 540 in channel 2, ROX, TEXAS RED, and Cal Red 610 in channel 3, CY5 and Quasar 670 in channel 4, Quasar 705 in channel 5, and FRET chemistry in channel 6. •· Capacity to store up to 20 data files and capable of automatic archive/email of data files •· Functional and user friendly software - includes quick start wizards, email notification with attached data files, and advanced data analysis modules, with automatic standard curve generation and quantification of unknowns (absolute quantification), melt curve analysis, allelic discrimination (precision melt software), end point fluorescence analysis, gene expression analysis (up to 50 plates with flexible plate configuration), and BioGazelle's qBase+ analysis software. •· Flexible relative gene expression analysis including delta CT, 2-delta-delta CT, Pfaffl, and/or Vandesompele methods. •· Precision Melt Analysis Software •· Multiuser friendly - capable of multiple users to create profiles for saving preferences for plate and protocol setup, data analysis, file folder use, and email addresses. •· Unlimited standard analysis software installation and use. •· Technical support - includes installation and training, training support CD, online trouble-shooting/technical support. •· Two year on-site warranty. Thermal Cycler Chassis C1000 Maximum ramp rate, °C/sec 5 Average ramp rate, °C/sec 3.3 Heating and cooling method Peltier Lid, °C Heats up to 105 Temperature Range, °C 0-100 Accuracy, °C ±0.2 of programmed target at 90°C Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C Gradient Operational range, °C 30-100 Programmable span, °C 1-24 Optical Detection Excitation 6 filtered LEDs Detection 6 filtered photodiodes Range of excitation/emission wavelengths, nm 450-730 Sensitivity Detects 1 copy of target sequence in human genomic DNA Dynamic range 10 orders of magnitude Scan Time All channels, sec 12 FAM/SYBR ® Green only, sec 3 Software Operating systems Windows XP, Windows Vista, Windows 7 Multiplex analysis Up to 5 targets per well System Licensed for real-time PCR Yes Sample capacity, wells 96 Sample size, µl 1-50 (10-25 recommended) Communication interface USB 2.0 Electrical approvals IEC, CE Dimensions (W x D x H), cm/in 33 x 46 x 36 /13 x 18 x 14 Weight, kg/lb 21/47
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USDA/ARS/PWAPh/AG81J8S11AA20/listing.html)
- Place of Performance
- Address: USDA-ARS, 21881 N Cardon Lane, Maricopa, Arizona, 85138, United States
- Zip Code: 85138
- Zip Code: 85138
- Record
- SN02497110-W 20110715/110713234726-0b0b3339d123c839bb498dac67b1aa4a (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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