SOLICITATION NOTICE
R -- READ HISTOSLIDES OF ACROPORID TISSUES
- Notice Date
- 7/21/2011
- Notice Type
- Presolicitation
- NAICS
- 541990
— All Other Professional, Scientific, and Technical Services
- Contracting Office
- Department of Commerce, National Oceanic and Atmospheric Administration (NOAA), Eastern Region Acquisition Division-KC, 601 East 12th Street, Room 1756, Kansas City, Missouri, 64106, United States
- ZIP Code
- 64106
- Solicitation Number
- WC133F-11-RQ-0899SLW
- Point of Contact
- Sharon L. Walker, Phone: (816)426-7470
- E-Mail Address
-
sharon.walker@noaa.gov
(sharon.walker@noaa.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- DESC: The U.S. Department of Commerce, National Marine Fisheries Service, Protected Resources Division in St. Petersburg, FL intends to award a Purchase Order to Dr. Ester C. Peters to read histoslides of acroporid tissues and development of a dataase of the tissue and zooxanthellae condition and the presence of several parasitic and pathogenic microorganisms and preparation of a manuscript of the findings. Dr. Peters is an aquatic toxicologist and pathobiologist with more than 35 years of experience in aquatic biology, as well as extensive environmental consulting, project management, quality assurance, and teaching experience. She has performed extensive work on the comparative histopathology of invertebrates and fishes, particularly carcinogenesis, as well as the relationships between adverse environmental conditions and diseases caused by pathogens and parasites. Dr. Peters, now an internationally recognized expert on comparative invertebrate histopathology who has published more than 40 articles in this field. No other coral reef scientist has this level of expertise in comparative histopathology and no other scientist has the extensive sets of histoslides and samples that Dr. Peters has collected of diseased and apparently healthy Acropora spp., nor has made arrangements with molecular and microbiology expert collaborators to work towards the goal of understanding why the Caribbean acroporids have been dying out since the early 1970s. The two species of Acropora that are listed as threatened under the ESA have been devastated by diseases since the late 1970s. Histoslides are created from tissue samples obtained from the corals. These samples have been taken from healthy and diseased corals from multiple locations ranging throughout their range over the past 40 years. The tissue samples are then processed and prepared for reading under a microscope, producing a histoslide. The samples have been collected from multiple locations over the course of 40 years and represent the disease or health condition of that particular coral at that particular moment in time. No one else has collected these tissue samples. Because we cannot recreate the conditions in the environment at the particular time the sample was taken, the collection of slides is not reproducible. Dr. Peters is the only known scientist to have collected the amount of samples at the spatial extend to provide a range-wide understanding of the potential pathogens or sources of coral diseases. Objectives • Reading of histoslides of acroporid tissues from the following collections: o Late 1970's Florida Keys o 1980-1981 Peters White Band Disease o 2003 Florida Keys o Smith Broward County o Sutherland o Kline Panama o Carne Belize o 2010 Key Largo • Development of a database of the tissue and zooxanthellae condition and the presence of several parasitic and pathogenic microorganisms • Preparation of a manuscript of the findings The results of this project will be used to inform the recovery of the ESA-listed corals. Tasks Task 1 - Preparation of histoslides from recently collected acroporid tissues The acroporid tissue samples recently collected will be prepared for reading by light microscopy. Task 2 - Reading of histoslides Histoslides of acroporid tissues will be read for tissue and zooxanthellae condition and the presence of parasitic and pathogenic microorganisms. Task 3 - Database development All data collected in Task 2 will be entered into a database for analysis. Task 4 - Manuscript preparation A manuscript of the findings of the study will be prepared. Deliverables Electronic copy of database and manuscript of the findings of the project. Special Material Requirements Tissue processing supplies and reagents including agarose, 10% EDTA solution, paraffin, hematoxylin and eosin, stains Place of Performance Performance will be at the Contractor's facility. The NAICS code for this action is 541990. Small business size standard shall not to exceed $7.0M. To be considered, firms must furnish detailed information concerning their capability to provide the services and satisfy or exceed the Government's needs. Such information shall include, at a minimum, name, telephone number and address of concerns for whom you have furnished this service previously, and detailed narrative that described your facility and ability and methods to provide this service to include resumes of the key personnel. Interested parties must respond within (15) fifteen days after publication of this notice. The government knows of only one source the Dr. Ester C. Peters that is capable of providing this service. The Eastern Region Administrative Division (ERAD), requires that all contractors doing business with the Acquisition Office must be registered with the Central Contractor Registry (CCR). No award can be made unless the vendor is registered in CCR. For additional information and to register in CCR please access the following website: http://www.ccr.gov/. In order to register with the CCR offerors must have a Dun & Bradstreet Number. A Dun & Bradstreet number may be acquired free of charge by contacting Dun & Bradstreet on-line at https://www.dnb.com/product/eupdate/requestOptions.html or by phone at (800) 333-0505. Faxed information can be sent to 816-274-6983 or physically sent Department of Commerce, Attn: Sharon Walker, 601 E. 12th St, Room 1756, Kansas City, MO 64106. Email responses can be sent to Sharon.walker@noaa.gov.
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