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FBO DAILY ISSUE OF AUGUST 26, 2011 FBO #3562
DOCUMENT

A -- RESEARCH STUDY OF BONE CONTENT AND METABOLISM - Attachment

Notice Date

8/24/2011
 

Notice Type

Attachment
 

NAICS

541712 — Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
 

Contracting Office

Department of Veterans Affairs;Network Contracting Activity 8;Commerce Building;300 E. University Ave. Ste. 180;Gainesville FL 32601-3460
 

ZIP Code

32601-3460
 

Solicitation Number

VA24811RI1283
 

Response Due

8/29/2011
 

Archive Date

10/28/2011
 

Point of Contact

Susan Dela Cruz
 

Small Business Set-Aside

N/A
 

Description

The Department of Veteran Affairs, NCA 8 NF/SG is seeking responsible sources to provide service as outlined in the Statement of Work. This is a market research for informational and planning purposes only. This is not a solicitation and does not obligate the government to issue a solicitation. This is to gain knowledge of potential qualified sources related to NAICS 541712. Please be advised that the Government will not pay for any information submitted under this announcement. Your response should identify your company name, point of contact with contact information, indicate all areas you are capable of providing service, capabilities statement and identify your business size, and if you are any of the following: Federal Supply Schedule holder, Small Business, 8(a) Disadvantaged, Woman-Owned, Veteran-Owned, Service Disabled Veteran Owned or HUB-Zone certified. Responses to this notice shall be e-mailed or faxed to Susan E. Dela Cruz, Contract Specialist, FAX 352- 381-5746; E-mail address: susan.delacruz@va.gov. Telephone responses will not be accepted. Responses must be received in writing no later than 12:00 p.m. EDT Aug 29, 2011. Responses should clearly state the ability to provide the service. If a solicitation is issued, it will be announced at a later date, and all interested parties must respond to that solicitation announcement separately. STATEMENT OF WORK Objective: The overall objective is to acquire professional services to assist in the execution of the project titled Bone Content and Metabolism of Sex Steroid Hormones. The primary scope of this service request will include: bone histomorphometric analysis and GCMS analysis of bone steroid hormones. Personnel Qualifications: The ideal contractor to perform the specifications will have a minimum qualifications equivalent to a PhD degree with good interpersonal, communication, and organizational skills. Research experience with assessment of bone mechanical strength is required. SCOPE OF WORK 1. Bone histomorphometric assessment Rat bones (tibia) are obtained at sacrifice by the laboratory of the VA Principal Investigator. Bones are stored frozen and delivered to the Contractor's Representatives. The Contractor's Representatives will analyze bones. The Contractor's Representative will also teach the above techniques to members of the laboratory of the VA Principal Investigator. Technical details: Bones are placed in 10% phosphate-buffered formalin (pH 7.4) for 24 hours before transfer to 70% ethanol and dehydration in increasing concentrations of ethanol. Sample bones are then embedded undecalcified in modified methyl methacrylate and sectioned longitudinally at 4 and 8 vm thickness with Leica/Jung 2050 or 2165 microtomes. The 4- µm-thick sections are stained by the Von Kossa method with a tetrachrome counterstain. Structural and cellular variables are measured in these sections with the Osteometrics System (Atlanta, GA and the Piquant Elite Bone Morphometry System (R&M Biometrics, Nashville, TN). The sample area within the proximal tibial metaphysis begins 1 mm distal to the growth plate to exclude the primary spongiosa. The bone variables listed below will be measured or calculated. Cancellous Bone Volume (%) Trabecular Width (pm) Trabecular Separation (pm) Node to Free End Ratio Osteoid Surface (%) Osteoclast Surface (%) Osteoid Volume (%) Trabecular Number (#/mm) Node to Node Ratio Free End to Free End Ratio Osteoblast Surface In addition, the following fluorochrome-based data will be collected from unstained, 8 thick bone sections with the same quantitative systems described above. Rate of Longitudinal Bone Growth (mm/day) Mineralizing Surface (%) Mineral Apposition Rate (mm/day) Bone Formation Rate (tissue level, surface referent, mm3/mm2/day) Each year of the contract, analysis will be performed on 84 rat bone specimens and 30 mouse bone specimens. 2. GCMS analysis of bone steroid hormones and establish chemical identity. Rat bones (femur), mouse bones (femur and tibia), and human bones (part of the fibula) are crushed and extracted by the laboratory of the VA Principal. Extracts are stored frozen and delivered to the Contractor's Representatives. The Contractor's Representatives analyze the hormone content of the extracts by gas chromatography, mass spectroscopy (GCMS) as follows. Technical details: To each extract, 300 pL of DMF and 200 pL of BSTFA with 1% TMCS are added, after which the solutions are vortexed, capped, and allowed to incubate for 20 min at 70 °C. The derivatized products are then taken to dryness under nitrogen, redissolved in 500 pL of acetonitrile, spiked with internal standard d12-pyrelene (from US-108) to a final concentration of 1 pg/mL, and analyzed via GCMS. As the additional solid phase extraction isolation steps (discussed previously) are determined, this method will change accordingly. Any samples that require deconjugation will be hydrolyzed prior to the first solvent evaporation that prepares the extracts for derivitization. From a single sample extract, estradiol, estrone, and estriol are assayed using an HP-6890 gas chromatograph (Hewlett-Packard, Palo Alto, CA) with split/splitless inlet operated in splitless mode at 260 °C. The sample is introduced in a 1-pL injection and separated across an HP-5MS column (30 m x 0.25 mm; 0.25-pm film thickness; J & W Scientific, Folsom, CA) under a temperature program that begins at 200 °C, is held for 2 min, then increases at 10 °C/ min to 300 °C with a 10-min hold. Estrogen detection is accomplished using an HP 5973 mass selective detector operated in electron impact mode with a source temperature of 230 °C and a quadrupole temperature of 150 °C. Identification of all analytes is conducted in full scan mode, where all ions are monitored. For improved sensitivity, selected ion monitoring is used for quantitation. The ions monitored for 1713-estradiol are m/z = 285 and 416, for estriol, m/z = 504 and 345, of d for estrone, m/z = 342 and 257. For quantitation, a standard curve containing at least six points is prepared for each analyte (R2 = 0.995). Calibration standards are prepared in acetonitrile and fresh curves are analyzed with each set of samples. Each year of the contract, analysis will be performed on 84 rat bone specimens, 30 mouse bone specimens and 12 human bone specimens. LOCATION OF CONTRACT PERFORMANCE The service analyses performed by Contractor Representative based upon GCMS analysis of bone steroid hormones and established chemical identity, and bone histomorphometric assessment will be conducted at Contractor's designated facility. It is contractor representative's responsibility to identify testing location suitable per above statement of work.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/VA/VANFSGHS/DVANoFSoGHS/VA24811RI1283/listing.html)
 

Document(s)

Attachment
 

File Name: VA-248-11-RI-1283 VA-248-11-RI-1283.docx (https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=240605&FileName=VA-248-11-RI-1283-000.docx)

Link: https://www.vendorportal.ecms.va.gov/FBODocumentServer/DocumentServer.aspx?DocumentId=240605&FileName=VA-248-11-RI-1283-000.docx

 

Note: If links are broken, refer to Point of Contact above or contact the FBO Help Desk at 877-472-3779.
 

Record

SN02548832-W 20110826/110825001256-507eaf95e4bdfed9fb7e44dc2b67bad0 (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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