SOLICITATION NOTICE
66 -- Reagents Requirements IDIQ - Base plus four option years
- Notice Date
- 11/22/2011
- Notice Type
- Presolicitation
- NAICS
- 325413
— In-Vitro Diagnostic Substance Manufacturing
- Contracting Office
- US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014
- ZIP Code
- 21702-5014
- Solicitation Number
- W81XWH12T0052
- Response Due
- 11/22/2011
- Archive Date
- 1/21/2012
- Point of Contact
- Colleen Barnes, 301-619-9599
- E-Mail Address
-
US Army Medical Research Acquisition Activity
(colleen.barnes@us.army.mil)
- Small Business Set-Aside
- N/A
- Description
- The United States Army Medical Research Acquisition Activity (USAMRAA) has a requirement in support of the U.S, Army Medical Research Institute of Infectious Diseases (USAMRIID). This requirement will be for a Requirements Indefinite Delivery Indefinite Quantity contract (IDIQ). The Period of Performance for this contract will be a base year with four options years. This is a Request for Quotation (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2001-27. This is an Unrestricted Competition. The North American Industry Classification System (NAICS) code is 325413. Quantities and prices are annual estimates. They are dependent on actual funding requirements, and the government is not obligated to purchase. Vendor shall provide products with a shelf life no shorter than six months from the date of the delivery. Under this contract, the customer places an order as required, and the vendor delivers as early as the next business day but no later than 5 business days. This award is for the reagents kits on an as-needed basis. The Minimum Essential Characteristics for this requirement are the following: Minimal Essential Characteristics for Genomics Sciences Sequencing Reagents for use on the Illumina Genome Analyzer IIx and HiSeq 2000 systems The following minimal essential characteristics are required: All of the below reagents are required to be licensed for use on the Illumina OR NAME BRAND OR EQUAL TO Genome Analyzer IIx and HiSeq 2000 system. Kit for cluster amplification on a paired-end flow cell: This kit is required to provide reagents for cluster amplification on paired-end flow cells using the cBot cluster amplification system. These reagents are to be provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation for use on a cBot cluster amplification system. The kit is required to include reagents for cluster resynthesis of the reverse strand on the Genome Analyzer IIx. Kit of reagents for high-throughput sequencing (36 cycle): This kit is required to be optimized for the Genome Analyzer IIx system. The kit is required to allow users to extend read lengths to 2 x 150 and achieve up to 95 Gb of mappable data. Reagents are to be ready-to-load on the Genome Analyzer systems. Kit of reagents for high-throughput sequencing (36 cycle) (20pk) : This kit is required to provide kitted, master-mixed reagents for 20 runs of 36 cycles of sequencing on the Genome Analyzer in a high-throughput form. Manifold for cluster generation on a flow cell: This manifold is required to provide for cluster generation on a flow cell for use on a cBot Cluster Generation system with a Genome Analyzer platform. Multiplexing Sequencing Primer and PhiX control Kit: This kit is required to have kitted multiplexing sequencing primers, control DNA, and buffers for 10 single read or paired end flow cells for use with the Genome Analyzer. 67 Prism with base: This prism is required to be compatible with use on a Genome Analyzer IIx System. DNA Sample Preparation Kit Set A: This kit is required to provide reagents for sample preparation of 48 DNA samples for single read, paired end, or multiplexing sequencing. Six unique indexes sufficient for eight individual samples are to be included in the kit. DNA Sample Preparation Kit Set B: This kit is required to provide reagents for sample preparation of 48 DNA samples for single read, paired end, or multiplexing sequencing. Six unique indexes sufficient for eight individual samples are to be included in the kit (different from Set A). TruSeq PE Cluster Kit v3 - cBot -HS: This kit is required to provide reagents that bind samples to complementary adapter oligos on the flow cell, enabling copying of the DNA strand after the first sequencing run. This copied strand is used to sequence the other fragment end. Clonal clusters of ~1000 copies each are required to be produced for sequencing. TruSeq SBS Kit - HS (200 cycle): This kit is required to provide reagents for up to 209 cycles of sequencing on the Illumina OR BRAND NAME OR EQUAL TO HiSeq sequencing system. This kit is required to contain ready-to-load reagents for sequencing each cluster on a flow cell using sequencing by synthesis technology. Multiplexing Sample Preparation Oligo Kit (96 samples): This kit is required to contain 12 unique oligonucleotides to tag libraries for pooling in one flow cell lane. Required to allow for up to 96 samples to be sequenced on a single flow cell using the Illumina OR BRAND NAME OR EQUAL TO Genome Analyzer. TruSeq SR Cluster Kit v3-cBot-HS: This kit is required to provide reagents for cluster amplification on single-end flow cells using the Illumina OR BRAND NAME OR EQUAL TO cBot cluster amplification system. The reagents are to be provided in a pre-mixed, 96-well plate format. This kit is required to enable up to 300 Gb of sequence output per run on the Illumina OR BRAND NAME OR EQUAL TO HiSeq 2000. TruSeq SR Cluster Kit v2-cBot-GA: This kit is required to provide reagents that bind DNA library samples to complementary adapter oligonucleotides attached on the flow cell surface. The reagents in this kit are required to be in a pre-mixed 96-well plate format. Diagnostics Systems Division Minimum Essential Characteristics I.Platforms utilized a.MiSeq personal sequencing system - This system utilizes sequencing by synthesis technology to sequence small genomes, transcriptomes, and other sequencing requirements. In this process, starting nucleic acid sample is reverse transcribed and/or fragmented, and sequencer-specific adaptors are ligated onto the fragments. Millions of labeled fragments are then sequenced using fluorescently labeled, reversible terminator-bound dNTPs. b.Genome Analyzer (GA) - This sequencing system is similar to the MiSeq, but there is significantly increased throughput of up to 95 gigabases with up to 640 million paired-end reads. Supplies required a. Library preparation kits - For sequencing, we require an Illumina-compatible library preparation kit. 1.TruSeq DNA and RNA Sample Prep Kits generate DNA libraries from DNA or RNA appropriate for Illumina-based sequencing. The resulting libraries are 300-500 base pairs for single, paired-end, and multiplexed sequencing. There are two sets with unique indices so multiple, separate libraries can be indexed for one sequencing run. The TruSeq Small RNA Sample Prep Kit is used to generate sequencable libraries from small RNA starting material. The TruSeq Dual Index Sequencing Primer Kit, Paired End, is used for sequencing dual index libraries. 2. Nextera DNA sample Preparation Kit and the accompanying Nextera Index Kits are also used to generate sequencable libraries. b. Reagents required for MiSeq system operation 1. We will also require reagents for sequencing the DNA/cDNA libraries we generate. The current anticipated sequencing cartridges are the 1) MiSeq 50-cycle Paired-End Kit, and the 2) MiSeq 300-cycle Paired-End kit. These cartridges would be compatible with the MiSeq and would contain the reagents required for sequencing appropriately generated DNA/cDNA libraries. c.Reagents required for the GA system operation 1.The TruSeq PE and SR Cluster Kits contain the reagents required for cluster generation and binding of the DNA library to complementary adapter oligonucleotides. The TruSeq SBS Kit contains the reagents required for 36 cycles of sequencing on the GA. Additional kits can be added during the sequencing run for longer sequencing reads. Specific Items Required: Library preparation kits - For sequencing, we require an Illumina-compatible library preparation kit. TruSeq DNA and RNA Sample Prep Kits generate DNA libraries from DNA or RNA appropriate for Illumina-based sequencing on the MiSeq platform. The resulting libraries are 300-500 basepairs for single, paired-end and multiplexed sequencing. There are two sets, A and B, with unique indexes so multiple, separate libraries can be indexed for one sequencing run. The TruSeq Dual Index Sequencing Primer Kit is used for sequencingdual index libraries. TruSeq small RNA Sample Prep Kits are used to generate DNA libraries from small RNAs. Nextera DNA sample preparation kits are also used to generate libraries using transposons. The Nextera Index kits contain unique indices in order to label individual samples for multiplexed sequencing runs. These reagents must be compatable with the Illumina MiSeq system for which they will be used. Reagents required for MiSeq system operation We will also require reagents for sequencing the DNA/cDNA libraries we generate. The current anticipated sequencing cartridges are the 1) MiSeq 50-cycle Paired-End Kit, and the 2) MiSeq 300-cycle Paired-End kit. These cartridges would be compatible with the MiSeq and would contain the reagents required for sequencing appropriately generated DNA/cDNA libraries. These reagents must be compatable with the Illumina MiSeq system for which they will be used. The TruSeq PE and SR Cluster Kits contain the reagents required for cluster generation and binding of the DNA library to complementary adapter oligonucleotides. The TruSeq SBS Kit contains the reagents required for 36 cycles of sequencing on the GA. Additional kits can be added during the sequencing run for longer sequencing reads. All responsible sources may submit a quote, which shall be considered by the agency. The competitive solicitation, solicitation amendments and all questions and answers relating to this procurement shall be made available via the Internet at https://acquisition.army.mil/asfi/ or https://www.fedbizopps.gov. The solicitation is anticipated for release to the vendor community on or about, November 22, 2011, and will only be available until December 22, 2011. Specifications of the requirement are provided in solicitation: W81XWH-12-T-0052. Potential offerors are responsible for accessing the website. Interested parties must respond to the solicitation in order to be considered for award of any resultant contract. To be eligible for an award, offerors MUST be registered with the Central Contractor Registry at https://ccr.gov/ and may have completed its representations and certification found at https://orca.bpn.gov/. All questions should be addressed in writing to Colleen Barnes, Contract Specialist at colleen.barnes@us.army.mil. Questions shall be submitted not later than 1200 hours (Eastern Day Light Savings Time) on December 6, 2011. Only one set of questions shall be received from each offeror. Numerous emails from one offeror shall not be accepted. Telephone requests will not be honored and bidder lists will not be maintained. All interested parties shall provide their quote to Colleen Barnes at colleen.barnes@us.army.mil by 10:00 a.m. ET on December 22, 2011.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/W81XWH12T0052/listing.html)
- Place of Performance
- Address: US Army Medical Research Acquisition Activity ATTN: MCMR-AAA, 820 Chandler Street Frederick MD
- Zip Code: 21702-5014
- Zip Code: 21702-5014
- Record
- SN02627482-W 20111124/111122234532-3589e262400ce1912719ccb0c9ba3318 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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