SOLICITATION NOTICE
66 -- Zeiss LMS 780 Confocal Microscope
- Notice Date
- 4/9/2012
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Blvd., EPS Suite 600, Rockville, Maryland, 20852
- ZIP Code
- 20852
- Solicitation Number
- N02RC26001-88
- Archive Date
- 5/3/2012
- Point of Contact
- Melissa R. Ward, Phone: 301-402-4509, Caren N Rasmussen, Phone: (301) 402-4509
- E-Mail Address
-
wardmr@mail.nih.gov, cr214i@nih.gov
(wardmr@mail.nih.gov, cr214i@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- Contracting Office Address Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 6120 Executive Boulevard, EPS Suite 600, Room 6072, Rockville, MD 20852, UNITED STATES. Description The National Cancer Institute (NCI), Center for Cancer Research (CCR), Laboratory of Experimental Carcinogenesis, plans to procure the brand name Zeiss LSM 780 Confocal Miscroscope manufactured by Carl Zeiss Microscope LLC, One Zeiss Drive, Thornwood, NY 10594. This acquisition will be processed under FAR Part 12 - Acquisition for Commercial Items and will be made pursuant to the authority in FAR 15, Contracting by Negotiation. The North American Industry Classification System Code is 334516 and the business size standard in 500. HHSAR 352.223-71 Instruction to Offerors - Sustainable Acquisition Offerors must include a Sustainable Acquisition Plan in their technical proposals that describes their approach and the quality assurance mechanisms in place for applying FAR 23.1 - Sustainable Acquisition Policy (and other Federal laws, regulations and Executive orders governing green purchasing) to this acquisition. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. Delivery: Approximately 14-16 weeks after receipt of award; installation within one week of delivery. The contractor shall provide: 1) one Zeiss LSM 780 Confocal Microscope 34 Channel System (410900196865000) with Scanning Stage and Environmental Chamber; PC computer, 30" monitor, and ZEN 2011 software; and an additional ZEN 2011 software dongle for the pre-existing computer workstation; 2) Complete confocal system must be accommodated in a room measuring 6 ½ ft x 9 ft with adequate space for proper air flow and sitting space for two users of the system. 3) 405 cw Laser Kit (4109009120830000): 4) 594 Laser Kit LSM 710 with 561nm (4109009120820000); 5) System table ELYRA (0000001954842000); 6) ZEN Module 3D VisArt Hardware License Key (4101361024110000); 7) ZEN Module FRET Hardware License Key (4101361011110000): 8) ZEN Module FRAP Analyses Hardware License Key (4101361010110000); 9) ZEN Module Physiology Hardware License Key (4101361019110000); 10) ZEN 2011 Experiment Designer/MT Package (4109009154000000); 11) Zen Mod.FCS GAaSP Hardware License Key (4101361004110000); 12) ZEN desk 2011 US Hardware License Key (4101351104110000); 13) Switching mirror MOT 2x, T-PMT (0000001269455000); 14) T-PMT (0000001410107000); 15) LSM adapter SP-R and T60N-L (42515800000000000); 16) Tube lens R 3.5 (0000001226198000); 17) Binoc Tube 45/23 f/Axio Observer (4255370000000000); 18) Equip laser safety Axio Observer/LSM (4329240000000000); 19) Fastening Kit for Inverted (0000001299421000); 20) Cable set for LSM 710 (0000001479586000); 21) APO Calibration LSM M27 (4206399000700000); 22) Reflector turret 6x mot ACR LSM (4249159010000000); 23) Transmitted light Illuminator LSM (42392300000000); 24) Cable CAN Bus 3.5m (0000001315568000); 25) Eyepiece PL 10x/23 Br foc (444036900000000); 26) X-Cite 120 PC with 1.5m Liquid Light Gu (0000001325338000); 27) Contact Kit Laser Safet X-Cite (0000001308905000); 28) Illumination Adapter HXP/X-Cite (423302000000000); 29) AV4 Module Autofocus (0000001235856000); 30) AV4 Module Mark and Find 2 (0000001304587000); The contractor shall provide on-site user training, toll-free technical support and one-year comprehensive (parts and labor) warranty, and on-site installation. NCI needs to purchase a fourth confocal and image processing system to meet the increased demand for extended time lapse imaging (overnight), to provide GAaSP 34 Channel spectral detector to provide greater sensitivity for low level fluorescent samples. This system must provide high resolution; expanded fluorescence capability; speed and ease of use; and quality image capture, processing, and storage capabilities. The Zeiss LSM 780 Confocal Microscope with Scanning Stage and Environmental Chamber will best meet all of NCI's specifications in one integrated system. This new confocal system must be compatible with NCI's existing equipment. As all of the current equipment is Zeiss, and PC-based, NCI must be able to network this new system to other computers and printers in the laboratory, thus allowing for easy image transfer and/or printing. NCI must be able to interchange parts, such as the PLAN APO objectives, if necessary. User training and implementation of the new system shall be rapid, minimizing any "down time." And this system must also be upgraded relatively easily and inexpensively in the future to add new capabilities as needed. NCI will be able to add this new confocal system to the existing discounted Zeiss preventive maintenance/service contract after the warranty expires, providing additional savings to the laboratory and the NCI in subsequent years. Because the Zeiss LSM 780 Confocal Microscope with Scanning Stage and Environmental Chamber has unique features not available from other known companies, is compatible with existing equipment, and will potentially yield additional savings to the laboratory and NCI in the future, it is the only known instrument able to meet the needs of NCI. The salient characteristics of the Zeiss LSM 780 system include: Microscope:  Computer controlled Metal Halide Fluorescence burner with variable intensity output.  Definite Focus - Integrated focus which maintains focus during long term live cell imaging. Features of High Sensitivity Laser Scanning Confocal Microscope:  Must Incorporate a 34-Channel Quasar(Spectral) GaAsP Detector, consisting of calibrated 32-Array GaAsp and two flanking single PMT detectors, enabling image acquisition with freely definable emission spectra and advanced spectral acquisition and analysis Spectral GaAsp detector allows increased QE(>45%) over standard PMT detectors, critical for our low light fluorescence applications.  System must include the following laser lines 405, 458,488,514,561,594,633  Hardware must support simultaneous acquisition from 34 channels plus TPMT and 2 additional detectors for up to 37 simultaneous 16 bit inputs.  Spectral GaAsP should allow image collecting in photon counting mode for single molecule applications or integration mode for standard fluorescence imaging.  Fluorescence Correlation Spectroscopy (FCS) to analyze single molecules with GaAsP detector. Up to 6 channels can be used in FCS mode, providing greater flexibility in stainings and samples.  Able to perform FCS analysis with an actively cooled, photon counting GaAsP detector.  Incorporates Primary Dichroioc Beam Splitter(Twin Gate) at 10 degree position, you achieve Extremely HIGH laser suppression on the order of 6-7 OD allowing superb reflection free imaging. The System omits all secondary dichroics for high efficiency emission collection.  Point Scanner must obtain at least 8 fps at 512x512 and e.g. 250 fps at 512x16  Digital gain (noise free contrast enhancement prior to bit depth conversion).in addition to standard master gain pedestal.  Free selection of emission bands for detection, omitting secondary dichroics or emission filters. Must be possible to set the emission bands over laser lines and still have efficient laser blocking to obtain superior signal to noise with low light fluorescent samples.  Freely rotatable scan field (0-360 degree in 0.1° steps) with user definable size and shape due to independent galvo-mirrors.  Real time electronics control offers more flexibility in managing image data in parallel WHILE ACQUIRING! Users can image process and review, not only previously collected images, but also the image data set currently being acquired without interfering with the image acquisition. Parallel image Acquisition and Analysis on the Confocal Platform is a requirement.  Multiple (up to 99) freely definable real regions of interest (rROIs). Pixel precise control of the laser intensity and signal detection. Prevention of photo damage outside the rROIs boundaries necessary for work on living cells, FRAP, uncaging, photoactivation and photoconversion experiments.  Individual AOTF settings (line selection and attenuation) per rROI.  Use up to 8 laser lines simultaneously with full power possible from each laser line.  System must offer at least 14 different scan speed levels (28 levels including bi-directional scans) for the wide variety of specimens and applications we will design. This is especially critical for our single molecule experiments.  It must be possible to select different scan speeds for bleach ROIs and image frame.  Variable scan field size (hardware-zoom) from 0.6 to 40 in 0.1 steps for magnifications until the optical limits of resolution, with a scan field of 20 mm diagonally in the intermediate image plane.  Image format from 4x1 to 6144x6144 pixels in up to 35 detection channels simultaneously with up to 16 bit depth (65536 gray values) in all channels. (8, 12 OR 16 bit user selectable bit depth.)  Spline scan for excitation and data acquisition along a curved line, essential for measurements along biological samples (e.g. cell membranes, cell processes).  Multitracking for the prevention of cross-talk by linewise or framewise switching of the laser excitation line, essential for multi-fluorescence applications and co-localization studies.  Automatic brightness compensation during the acquisition of z stacks by stepped incrementation of the excitation (laser intensity) and/or the detection gain (detector sensitivity). Must support at least a 5 point incrementation.  Advanced time-series module with automatic bleach and direct trigger-control to design the entire experimental protocol. All scan strategies can be applied (line, spline, frame, z-line, z-spline, z-stack, rROIs, z-rROIs) in the time series.  Absolute linear scanner movement to ensure equal pixel dwell-times as a prerequisite for any quantitative studies.  Calibration and linearization of the 32-Array GaAsp detector and the two flanking single PMTs, all 34-channels can be used for spectral acquisition and analysis.  34-Channel GaAsP detector (GaAsp-array with 32 PMTs and two flanking single PMTs) in combination with the highly efficient holographic grating to provide an even spectral dispersion over the entire visual spectral range.  Spectral recycling loop efficiently recovers non-separated light, redirecting it onto the holographic grating.  Fast parallel acquisition in one single scan of the entire spectral range as a lambda stack (391-749 nm) covered by the 34-Channel QUASAR Detector. (Suitable for spectral analysis in living cells, e.g. colocalization studies and FRET-experiments). Up to 10 different fluorescent dyes can be imaged and separated simultaneously.  Spectral bandwidth for lambda stack acquisition must be selectable by the user. A full spectral image is used for unmixing (suitable for spectral imaging of living cells and later unmixing without any spectral distortions).  Reproducible high resolution spectral data acquisition with 3.2 nm or 4.9 nm steps through sequential spectral scanning in addition to the 10nm parallel spectral scanning.  Online Fingerprinting: Image channel definition not by emission bands but by reference spectra and live processing unmixing during image acquisition.  Spectra can be created from a Lambda stack.  Free combination of manually defined, automatically extracted and spectra stored in the database is possible to perform unmixing on a lambda stack.  Potential to use the entire emission spectrum for dyes for detecting with much higher signal to noise ratios (in contrast to detection with bandpass techniques).  Optics are designed for high transmission and aberration free imaging for 350 - 1100 nm.  Pigtailed visible Lasers, are easily upgradeable. Plug and play bayonette mounting for easy maintenance and reliable, reproducible operation. No mirror alignment required for lasers.  Quantitative Colocalization is a part of the basic software with an interactive link between image display, scattergram and data table. The software includes interactive or automatic determination of thresholds, quantitative colocalization analysis with parameters like number of pixels, mean intensities +/-SD, colocalization coefficients, weighted colocalization coefficients, overlap coefficient after Manders, correlation coefficients, export of the analysis result and extraction of the colocalizing structures e.g. from image stacks.  Transmitted detector must be included. Combination of a transmitted-light illuminator (e.g. a halogen lamp) with one of the detectors, fluorescent images can be superimposed with non-confocal transmitted light images. Software Options:  Quantitative Physiology package for acquisition of ion indicator dyes and display and analysis of ion concentrations including online and offline ratio for ratiometric dyes, online and offline F/F0 and DF/F for single wavelength dyes, calibration for single-wavelength and ratiometric dyes in situ and in vitro including background correction (after titration and with various curve fits or after Grynkiewicz).  FRET and FRAP acquisition and analysis must be software integrated  Multiple Time Series software package to permit complex, combined time series with changing application configurations, autofocus and bleach functions.  Tiling and Multiposition Scanning fully integrated into Software  Smart Setup Software required for easy hardware control to adapt system configuration according to chosen dyes from a large database of fluorochromes.  Optimal hardware settings for Channel unmixing are required within the Smart Setup Software tool.  System must include self-test tool, with an easy to use maintenance user interface in combination with a calibration objective for automated pinhole adjustment and automatic scanner calibration, securing optimal system performance.  Provide innovative concept for one touch button for reproducibility of experiments for multi-user environments via a REUSE function allows reactivation of all acquisition parameters necessary to reproduce an experiment (each image stores the entire method). This is not a solicitation for competitive proposals. However, if any interested party believes they can meet the above requirement, they may submit a statement of capabilities. All information furnished must be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the above unique specifications described herein. An original and one copy of the capability statement must be received in the NCI Office of Acquisitions on or before 11:00 AM EST on April 18, 2012. No electronic capability statements will be accepted (i.e. email or fax). An original and one copy must be sent to the NCI Office of Acquisitions at the address stated above. All questions must be in writing and can be faxed (301) 402-4513 or emailed to Melissa Ward, Contract Specialist, at wardmr@mail.nih.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the Central Contractor Registration (CCR) www.ccr.gov and the Online Representations and Certifications Applications (ORCA), http://orca.bpn.gov. No collect calls will be accepted. Please reference solicitation number NCI-120046-MW on all correspondence.
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