SOURCES SOUGHT
B -- Field Sample Collection Services to be provided in Ohio
- Notice Date
- 3/12/2013
- Notice Type
- Sources Sought
- NAICS
- 541990
— All Other Professional, Scientific, and Technical Services
- Contracting Office
- USGS OAG DENVER ACQUISITION BRANCHPO BOX 25046204 DENVER FEDERAL CENTERDENVERCO80225-0046US
- ZIP Code
- 00000
- Solicitation Number
- G13PS00258
- Response Due
- 3/16/2013
- Archive Date
- 4/15/2013
- Point of Contact
- Lisa Hayes
- E-Mail Address
-
ldhayes@usgs.gov
(ldhayes@usgs.gov)
- Small Business Set-Aside
- N/A
- Description
- Sources Sought Notice for Small Business Search (Please see attached document for details). The U.S. Geological Survey (USGS) Ohio Water Science Center has a requirement for services to be provided for field sample collection at four sites in the western Lake Erie area: Maumee Bay State Park Lake Erie Beach, Maumee Bay State Park inland beach, Lions Park (Sandusky), and one additional site on Sandusky Bay or Lake Erie. In Ohio, local health officials have identified the presence of harmful algal blooms (HABs) during the summer and early fall seasons at some Lake Erie and inland lake beaches. The HABs are caused by cyanobacteria that produce microcystin and other toxins. The overall goal of the project is to apply new tools over an entire season to better understand the link between cyanobacteria community structure, environmental and water-quality factors, and HAB toxicity. The new tools include (1) assays for a molecular method, quantitative polymerase chain reaction (qPCR), that target the genes for cyanobacteria and toxin producers and (2) optical sensors based on the fluorescent properties of chlorophyll (indicative of total phytoplankton abundance) and phycocyanin (indicative of cyanobacterial abundance). The USGS is seeking small business sources to provide the following services: Scope of Work: This scope of work is for the collection of field data and samples from May through December, 2013, by the contractor and includes the following elements: 1. Eight sampling trips throughout the season on days agreed upon between the contractor and the USGS project chief. Some sampling trips will be scheduled to target HABs. A tentative schedule is one sampling trip in May, one in July, two in August, two in September, one in October, and one in November. 2. Samples will be collected at the following sites during each trip: - Maumee Bay State Park Lake Erie beach - Maumee Bay State Park inland beach (control site not generally affected by HABs) - Two additional sites on Sandusky Bay or western Lake Erie. Potential sites include Lions Park (Sandusky), Bay View West (Sandusky), East Harbor State Park, and Port Clinton. 3. During each sampling trip the following constituents will be measured: - Specific conductance, dissolved oxygen, pH, temperature, chlorophyll, and phycocyanin at the time of sampling using an in-situ probe. The USGS will provide equipment for all these measurements. The contractor is expected to maintain and calibrate equipment per manufacturers instructions, and record calibrations in a log book. - In addition to measurements during the 8 sampling trips, these measurements will also be made regularly (weekly or more often) throughout the season. - Secchi depth at the time of sampling. The USGS will provide equipment, if needed. - The contractor will completely and accurately fill out field observations on field forms (provided by the USGS) at each site during each sampling trip. - At each beach, a sample is collected from six locations (three transects at knee- and hip-deep water) within the designated swimming area and composited into one sample. If an accumulation of cyanobacteria occurs outside of the transect location, an additional sample will be collected from the accumulation. - The composited sample will be split into aliquots for different analyses. All bottles, preservation supplies, filtering equipment and supplies, and shipping materials will be provided by the USGS. Analytical service request forms (ASRs) will be filled out for each sample type (provided by the USGS), keeping a copy for the file and shipping the original paperwork with the sample. - Nutrients. Samples filtered and preserved on ice and shipped to the USGS National Water Quality Laboratory, in Denver, Colo. on ice within 48 hrs of sampling. - Cyanotoxins (microcystin, saxitoxin, and cylindrospermopsins by ELISA (total). Two 100-mL samples are frozen (total toxins) within 24-hours of collection. One sample from each pair is shipped on dry ice to the USGS Kansas Organic Geochemistry Laboratory. Samples are shipped twice during the sampling period. -Phytoplankton abundance and community structure by microscopy. Samples are preserved with lugols solution and stored in the refrigerator. They are shipped in a box to BSA Environmental in Cleveland, Ohio, twice during the sampling period. - Cyanotoxins by liquid chromatography-mass spectrometry (LC-MS). This will be done for approximately 25 percent of samples if a more detailed analysis is warranted (i.e., the phytoplankton community structure consists of anatoxin producers). Samples are processed and preserved as described above for ELISA. - Cyanobacteria by qPCR. Three sample aliquots of approximately 30-mL are filtered in the field or within 15 min of sampling, stored on dry ice during transport, kept frozen in the laboratory, and transported on dry ice by personnel from the USGS to the USGS Ohio Water Microbiology in Columbus, Ohio. Samples will be transported to Columbus twice during the sampling period. Training in field data collection methods and sample processing will be provided by the USGS Ohio Water Science Center. Regular QA/QC performance checks will be conducted by USGS Ohio Water Science Center staff on contractor's personnel throughout the specified project timeframe. The USGS is in search of small business firms who are able to provide the above mentioned service. Small business firms who feel they can furnish the required service or its functional equivalent (based on criteria specified herein) are invited to submit in writing an affirmative response to this announcement. An affirmative response would include literature, brochures and such other materials, which correspond to the required supply/service stated herein. This information will be used to determine if competitive opportunities exist. Since no solicitation document exists, request for such documents without accompanying information will be considered non-responsive to this request. POC: Lisa D. Hayes, U. S. Geological Survey, P. O. Box 25046, MS-204, Denver, CO 80225, e-mail: ldhayes@usgs.gov
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/DOI/USGS/USGS/G13PS00258/listing.html)
- Record
- SN03010036-W 20130314/130312235214-d57e93ca992b6b54fcd2e8e785dad195 (fbodaily.com)
- Source
-
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