SOURCES SOUGHT
66 -- Confocal Multi-photon Imaging Detection System
- Notice Date
- 5/10/2013
- Notice Type
- Sources Sought
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIDA-SSSA-SS-13-0509
- Archive Date
- 6/1/2013
- Point of Contact
- Hunter A. Tjugum, Phone: 301 435 8780
- E-Mail Address
-
tjugumha@mail.nih.gov
(tjugumha@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The National Institute of Drug Abuse (NIDA), on behalf of the National Institute of Neurological Disorders and Stroke (NINDS), seeks to obtain information regarding the availability and capability of all qualified sources to provide the potential requirement described in this announcement. The purpose of this sources sought is to seek capability statements from qualified small businesses (e.g. 8(a), veteran-owned small business, services-disabled veteran owned small business, HUB Zone small business, small disadvantaged business, or women-owned small business) having the capabilities to provide an advanced microscope imaging system with the technical specifications listed below. Based on the responses received from this Sources Sought announcement, the proposed acquisition may be solicited as a Total Small Business Set-Aside. All eligible small business concerns responding to this announcement must have the capabilities to provide the below product within 120 days after receipt of order. Delivery will be made to NINDS located on the NIH main campus in Bethesda, MD. The Dendrite Morphogenesis and Plasticity unit at NINDS is in need of a confocal multi-photon imaging system to perform in-vivo and in-vitro analyses of the central nervous system in Drosophila. System needs to be capable of acquiring and analyzing high resolution confocal images for both morphological and physiological studies, where high sensitivity, resolution and flexibility are critical. The advanced imaging system needs to fulfill three (3) technical specifications, required to perform experiments on three different preparations serving the following distinct purposes: 1. Confocal/Multi-photon microscope with near infrared laser (NIR) Purpose is to perform experiments on brain tissues or whole animals expressing various fluorescent proteins and calcium indicators. They can be directly imaged with the aid of an NIR laser, which is necessary for low damage to the live tissue and deep penetration. The NIR multi-photon microscope will be used for morphological as well as physiological studies in live tissues. Technical Specifications must include: A fixed stage upright microscope with upper and lower body built as 2 separate components allowing large working distance for specimens-up to 100mm; With ability to accommodate 75mm and 45mm focal distance optics; IR illumination built into the transmitted light path; Area and control for Dodt contrast slider integrated into transmitted light base for IR/VIS Dodt contrast technique 34-PMT Quasar (Spectral) GaAsP detector, consisting of calibrated & linearized 32-Element GaAsP PMT and two flanking single PMT detectors; Hardware must support simultaneous acquisition from 34 channels and 2 additional detectors for up to 36 simultaneous 16 bit inputs. Field upgradable to include one near infared laser (NIR) or one NIR laser + OPO or multiple NIR lasers; Must incorporate Primary Dichroioc Beam Splitter at 10 degree position; Point scanner must obtain at least 8 fps at 512x512 pixels and e.g. 250 fps at 512x16 pixels; Must have Digital Gain (noise free contrast enhancement prior to bit depth conversion), in addition to standard mater gain pedestal Freely rotatable scan field (0-360 degree in 0.1° steps) with user definable size and shape due to independent galvanometer-driven mirrors; Parallel image acquisition and analysis; Multiple (up to 99) freely definable real regions of interest (rROIs); Different scan speeds for bleach ROIs and image frame; Variable scan field size (hardware-zoom) from 0.6 to 40 in 0.1 steps for magnifications; a scan field of 20 mm diagonally in the intermediate image plane; System must offer at least 14 different scan speed levels (28 levels including bi-directional scans) for the wide variety of specimens and application; Multi-tracking for the prevention of cross-talk by linewise or framewise switching of the laser excitation line; Automatic brightness compensation during the acquisition of z stacks by stepped incrementation of the excitation (laser intensity) and/or the detection gain (detector sensitivity). Must support at least a 5 point incrementation; Advanced time-series module with automatic bleach and direct trigger-control to design the entire experimental protocol. All scan strategies can be applied (line, spline, frame, z-line, z-spline, z-stack, rROIs, z-rROIs) in the time series; Absolute linear scanner movement to ensure equal pixel dwell-times as a prerequisite for any quantitative studies; Optics designed for high transmission and aberration free imaging for 350 - 1100nm, optimized for both single and multi-photon confocal imaging; Optics must include LD 40X/NA 1.1, c-Apochromat; 20X/NA 1.0 W Plan-Apo, 63X/NA 1.4, 20X/NA 0.8, 10X/NA 0.3; Must include a suitable airtable for the system 2. Confocal microscope with high speed and sensitivity This microscope will be used for fixed tissues on slides. The dissected brain tissues from the animal can be fixed, followed by immunohistochemistry analysis using fluorescent labeled antibodies. A confocal microscope with high speed and sensitivity will be used to collect images of optical sections from these fixed samples. Technical Specifications must include: Spectral data collection down to 1nm resolution; Emission Fingerprinting - reliable separation of different fluorescent dyes; Variable Secondary Dichroic beam splitter (VSD) Optical continuous spectral element; Low noise electronic design allowing Calibrated Linear Gain boost; Software/Hardware Flexibility which allows management of data online and simultaneous data analysis while images are being collected, in support of parallel collection and analysis of data System must include the following laser lines: 488, 561,633 Line by line switch multi-tracking of 3 channels must be possible with the system Primary Dichroics must provide 6-7 OD blocking of laser - High laser suppression for near-reflection free imaging with low angle (~15°) dichroics; Freely rotatable scan field of 360 degrees with all lasers and freely definable center offset for the scan field; with full 360 degree rotation; Line selection and attenuation per ROI of at least 99 ROI's; High temporal color resolution for 3 colors in support of line switch to 2 detector channels; 0.6 to 40x Zoom capability; Absolute linear scan movement to insure equal pixel dwell times; Optics must be compatible with lens obtained for multi-photon imaging system; Must include a suitable airtable for the system. 3. Fluorescent stereo scope This stereo scope will be used for both the larva and the adult in whole animals. A fluorescent stereo scope will be used to image, sort and dissect these animals labeled with fluorescent markers. Technical Specifications must include: Must have a total zoom range of 20x Must have the maximum resolution up to 1000 line pair/mm resolution; Uses X-Cite 120 Illumination with long lifetime lamps (1500-2000 hours) with no alignment; Must have High Quality Filters (HE Filters) for GFP, RFP Must have large field of view, up to 23mm; Must have the Deep 3-D imaging capability with wide base and maximal possible aperture of the front lens; Photo Ergo Tube with variable angle and integrated documentation port; Must have fully motorized zoom optics with automatic calculations of Depth of Field, Magnification, Field of View and Resolution; Human Interface Panel (HIP) with autonomous control of motorized components, reproducible parameters and easy focus and/or zoom adjustments via a progressive wheel, 2 memory functions and real time display of magnification and Field. Interested parties should submit a tailored capability statement, which clearly defines the ability to provide this equipment, limited to 15 pages in length, including attachments, as an Adobe PDF or Microsoft Office file to: tjugumha@mail.nih.gov prior to the closing date in this announcement. Responses must include the respondents' DUNS, organization name and address, point of contact, and size and type of business pursuant to the North American Industry Classification System (NAICS) code 334516 with size standard of 500 employees. Disclaimer and Important News: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization's qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a pre-solicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).
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