MODIFICATION
66 -- HiSeq 2500 Sequencing System
- Notice Date
- 5/20/2013
- Notice Type
- Modification/Amendment
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NIH-NHLBI-CSB-(HG)-2013-114-DLM
- Archive Date
- 6/22/2013
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS PRE-SOLICITATION IS BEING AMENDED TO REVISE THE LAST PARAGRAPH, ADDITIONAL INFORMATION AND THE CLOSING DATE OF THE SOLICITATION. INTRODUCTION : THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR A PROPOSAL. A SOLICITATION DOCUMENT WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) on behalf of the National Human Genome Research Institute, (NHGRI), intends to negotiate and award a purchase order on a noncompetitive sole source basis to Illumina Inc., 9885 Towne Centre Drive, San Diego, California 92121-1975. Procurement : 1. HiSeq 2500, Catalog Number: SY-401-2501, Quantity: One (1). 2. Shipping and Insurance. BACKGROUND : The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency conducting and supporting medical discoveries that improve people's health and save lives. The National Institute of Health (NIH), National Human Genome Research Institute (NHGRI), NIH Intramural Sequencing Center (NISC) is a high-throughput sequencing center supporting all institutes within the NIH. NISC has served the NIH community for over 15 years as a source for high-quality large-scale sequencing and analysis. As next generation sequencing applications expand, so does the demand for NISC services. NISC currently has three (3) MiSeq Systems, three (3) Illumina's Genome Analyzer (GA) IIx, and three (3) HiSeq 2000 Systems which are proven platforms for genetic analysis and functional genomics. This instrument's parallel sequencing technology leverages clonal cluster formation and proprietary reversible terminator chemistry to give it the speed and reduce the cost of large-scale sequencing to generate billions of bases of high-quality DNA sequence per run. The HiSeq 2500 is compatible with the existing MiSeq, Cbot Cluster, Genome Analyzer IIx and the HiSeq 2000 that are currently being used in the Sequencing Center. Maintaining compatibility is a must and essential. The HiSeq 2500 System provides ultra-high output and speed. In a single run one is able to sequence more than 5 human genomes at ~30x coverage. The HiSeq 2500 system is able to process a diverse set of samples within one flow cell, and with the highest data quality currently available. Another key factor in this selection is that this instrument is able to run in an alternate rapid mode which would allow the researchers to decrease turnaround time by many weeks. This is a critical consideration for projects impacting patient diagnosis. The HiSeq 2500 can be switched between a high throughput mode and rapid turnaround run mode. In high throughput run mode, similar to the HiSeq 2000, the HiSeq 2500 enables sequencing of a large number of genomes, exomes, or transcriptomes in a single run, allowing large studies to be completed with the fewest number of runs. In rapid run mode, the HiSeq 2500 is capable of sequencing a 30x genome in a day or completing fast multiplexed applications such as exome resequencing or RNA-Seq. The rapid run time includes automated, on-instrument cluster generation, and paired-end sequencing, with no user intervention needed throughout the entire process. The HiSeq 2500 also allows researchers to process samples by a single method that is compatible with HiSeq 2000 and HiSeq 2500. This simplifies the NISC lab processes, minimizing labor and equipment costs, and gives researchers the flexibility to route samples to either machine type depending on the needs of the project and machine availability. The high speed of the HiSeq 2500, means that reagents sit out for a shorter period of time and decay to a lesser degree during the run. The result is longer, more accurate reads (300 vs 200 bases). This allows NISC to expand the sequencing applications supported on this platform, as some supplications require longer reads. PERIOD OF PERFORMANCE : Delivery of HiSeq 2500 will be delivered within 6-weeks of receiving the approved contract award. PROJECT DESCRIPTION: The NIH Intramural Sequencing Center (NISC), established in 1997, is a multi-disciplinary genomics facility that emphasizes the generation and analysis of DNA sequence. In addition to providing investigators at the NIH access to large-scale DNA sequencing, NISC plays a major role in several efforts related to the Human Genome Project including a Comparative Vertebrate Sequencing Initiative, the ENCODE Project, and the Mammalian Gene Collection Program. NISC is also an active participant in the International Sequencing Consortium. While it is currently feasible to sequence an entire genome it is quite expensive and unwieldy. When an investigator wishes to study only selected genes or genomic regions it is more cost-effective and efficient to perform targeted sequencing. Contractor Requirements : • Must be able to run the same chemistry as HiSeq 2000, MiSeq, and Genome Analyzer IIx Systems. • Run time for 200 base run must be under 30 hours. • Data should be able to be processed through NISC's data analysis software. • Preparation time for samples to load on the sequencer should take about a day. • Read length at least 150 bp. • Produce paired-end reads of 60 million per run. • Reagents are in convenient volumes and are ready-to-load. • Oil is not required for the optics. • Straightforward protocols that require no beads or emulsions. • System should be able to perform amplification, sequencing and data analysis in one unit. Government Responsibilities : Not applicable Reporting Requirements and Deliverables : Not Applicable Program Management and Control Requirements : Not applicable Inspection and Acceptance Requirements : • Upon arrival of equipment to destination, the package must be undamaged. • In the installation of the equipment, all parts including computer must be functional and running in proper order. REGULATORY AUTHORITY" This acquisition is conducted under the authority of 41 U.S.C. 253(c) as set forth in FAR Part 6.302-1, only one responsible source and no other supplies or services will satisfy agency requirements. ADDITIONAL INFORMATION: Industry Classification (NAICS) Code is 334516, Analytical Laboratory Instrument Manufacturing and the Small Business Size Standard is 500 Employees. The resultant Contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-66 (February 28, 2013). The determination by the Government to award a purchase order on a sole source on non-competitive basis for the procurement of a HiSeq 2500 Sequencing System to Illumina, Inc. is based upon Market Research conducted: no response was received on the sources sought synopsis published in the FedBizOpps for 15-days seeking small businesses under [HHS-NIH-NHLBI-CSB-2013-025-DLM, February 4, 2013, HiSeq 2500 (Brand Name: Illumina)]. An SBA Dynamic search was conducted and received no results for small businesses. A sole source letter was received from Illumina, Inc., stating: "Illumina has exclusive rights, US Patent # US 7,589,315 and foreign equivalents for the HiSeq 2500 including trade secret and other proprietary rights. Responses to this notice shall contain sufficient documentation to establish the interested parties' bona-fide capabilities for fulfilling the requirement. All responsible sources may submit a capability statement, proposal, or quotation, which shall be considered by the agency. This notice of intent is not a request for competitive proposals. Inquiries to this announcement, referencing synopsis number NHLBI-CSB-(HG)-2013-114-DLM, may be submitted to the National Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. Note: In order to receive an award from NIH/NHLBI, contractors must be registered and have a valid CCR and ORCA on-line at https://www.sam.gov/portal/public/SAM/.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NIH-NHLBI-CSB-(HG)-2013-114-DLM/listing.html)
- Place of Performance
- Address: NIH/NISC Intramural Sequencing Center, 5625 Fishers Lane, Rockville, Maryland, 20852, United States
- Zip Code: 20852
- Zip Code: 20852
- Record
- SN03066452-W 20130522/130520234416-8073d1f42a4138af35542989f226320d (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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