SOLICITATION NOTICE
Q -- Analysis of genes using Drosophila model systems
- Notice Date
- 7/2/2013
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541712
— Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHS-NIH-NHLBI-CSB-(HG)-2013-200-DLM
- Archive Date
- 7/24/2013
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- i. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. ii. The solicitation number is NHLBI-CSB-(HG)-2013-200-DLM and the solicitation is being posted as a request for proposal (RFP). Offerors shall submit their proposals no later than 7:30 a.m. Eastern Standard Time (EST) on July 9, 2013. iii. A statement that the solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-68, June 26, 2013. iv. The associated North American Industry Classification System (NAICS) code for this is 541712, Research and Development in the Physical, Engineering, and Life Sciences (except biotechnology) and small business size standard is 500. A. Background : The National Institute of Health (NIH) is the nation's leading medical research agency and the primary Federal agency conducting, supporting and making medical discoveries that improve people's health and save lives. National Institutes of Health Undiagnosed Disease Program (UDP) is a trans-National Institute of Health (NIH) initiative that focuses on the most puzzling medical cases referred to the NIH Clinical Center in Bethesda, MD. It was organized by the National Human Genome Research Institute (NHGRI), the NIH Office of Rare Diseases Research (ORDR) and the NIH Clinical Center. Many medical specialties from other NIH research centers and institutes contribute the expertise needed to conduct the program, including endocrinology, immunology, oncology, dermatology, dentistry, cardiology and genetics, among the dozens of participating senior attending physicians. A longstanding medical condition that eludes diagnosis by a referring physician can be considered undiagnosed and may be of interest to this clinical research program. B. Purpose and Objectives : The purpose of this requirement is to provide a cutting genetic and functional analysis of the role of mutant genes in the development and maintenance of the nervous system using Drosophila models. The analysis shall use a robust methodology for characterizing the neurological disease genes using Drosophila. C. Scope of Work : 1. General Requirements : A. A functional screening of sixteen (16) candidate genes identified in the Undiagnosed Disease Program will be carried out using Drosophila model systems to establish and analyze the loss-of-function phenotypes of the candidate genes identified in the National Human Genome Research Institute (NHGRI), Undiagnosed Disease Program (UDP) program. Each candidate gene will be functionally analyzed in Drosophila for the role of the encoded proteins in neurodevelopment and neurodegeneration. Description of Work Deliverables : 1. Obtain and establish loss-of-function mutant alleles of the Drosophila homologues of candidate genes (genes 1-8); Deliverables: Genomic information of Drosophila homologues Alleles information (1 to 2 RNAi lines per gene) (genes 1-10); Timeline: 1-13 Weeks. 2. Characterize gross and neurological phenotypes of the loss-of-function fly strains (genes 1-8); Deliverables: Data of phenotypic analyses (sample size: 2,000 to 4,000 flies) (genes 1-10); Timeline: 14-26 Weeks. 3. Obtain and establish loss-of-function mutant alleles of the Drosophila homologues of candidate genes (genes 9-16); Deliverables: Genomic information of Drosophila homologues, Alleles information (1 to 2 RNAi lines per gene) (genes 11-20); Timeline: 27-39 Weeks. 4. Characterize gross and neurological phenotypes of the loss-of-function fly strains; Deliverables: Data of phenotypic analyses (sample size: 2,000 to 4,000 flies) (genes 11-20)l; Timeline: 40-52 Weeks. B. Rescue analysis of candidate genes with known neurological phenotypes identified in UDP. Description of Work Deliverables : 1. Establish transgenic lines carrying human genes, wild type (1) and mutant alleles (1 to 2); Deliverables: Information on transgenic lines (2 transgenic lines per allele, 4 to 6 lines per gene); Timeline: 1-10 Weeks. 2. Recombine transgenic chromosome with RNAi chromosome to establish double transgenic lines (three-generation genetic crosses to establish 4 to 6 lines per gene; Deliverables: Information on double transgenic lines. Genotype: (1) UAS-WT;UAS-RNAi, (2/3) UAS-mut1/2;UAS-RNAi, (4) UAS-ctrl;UAS-RNAi); Timeline: 11-26 Weeks. 3. Establish global and neuronal specific loss-of-function fly strains; Deliverables: Information on loss-of-function alleles. (10 crosses will be set up per allele, 50 to 70 crosses per gene); Timeline: 27-39 Weeks. 4. Characterize gross and neurological phenotypes of the loss-of-function fly strains; Deliverables: Data of phenotypic analyses (sample size: 500 to 700 flies per gene); Timeline: 40-52 Weeks. 2. Specific Requirements : (a) Identify and obtain transgenic RNAi mutant alleles for the Drosophila homologue of the candidate genes (1 to 2 RNAi lines per gene, 10 to 20 RNAi lines in total for a 10-gene batch) (b) Establish global loss-of-function fly strains (10 to 20 crosses will be set up per gene, 100 to 200 crosses for a 10-gene batch) (c) Analyze the gross and systemic phenotypes including survival and lifespan (100 to 200 flies will be analyzed per gene, 1,000 to 2,000 flies for a 10-gene batch) (d) Establish nervous system specific loss-of-function fly strains (10 to 20 crosses will be set up per gene, 100 to 200 crosses for a 10-gene batch)Analyze neurological and behavior phenotypes including motor coordination (100 to 200 flies will be analyzed per gene, 1,000 to 2,000 flies for a 10-gene batch). (e) The Contractor shall supply electronic copies of all reports appropriate for deposition in the UDP process management system and for other collaborations. 3. Desired Results/Functions/End Items : (a) Results with qualified interpretations will be returned in a timely way. (b) Research collaborations will be conducted via Videoconferences, email communications, shared professional presentations, posters and publications. Grand Round presentations at the Contractor's location and the NIH Clinical Center may also occur. 4. Technical Specifications/Standards/Methods : The Contractor shall supply results with qualified interpretations in formats appropriate to the process management systems and for facilitation of other collaborations. 5. Special Handling or Handling Requirements : The Contractor shall provide result reports and record methods and materials as appropriate to the highest scientific standards. These materials, methods and results will be shared with agreed upon UDP collaborators. 6. Government Responsibilities: (a) The Government will not furnish any government property to the vendor (b) The Government will provide the names of the genes of interest to the Contractor. (c) The Government will not furnish any facilities to be used by the vendor. (d) The Government will be responsible for reviewing and approving reports and similar matters generated. The report results will be provided (1) electronically to the UDP via secure email, the UDP ftp site or direct deposition into the UDP process management system; and (2) will also be shared with UDP collaborators as defined mutually. 7. Reporting Requirements and Deliverables : (a) The Government will receive all electronic results within 26 weeks of notification for each ten genes in question. (b) The Government will receive additional reports for any test added-on after the Contractor receives the original specimen. D. Anticipated Period of Performance : Twelve (12) months from the date of award. v. NIH/UDP is located at 9 Memorial Drive, Bethesda, Maryland 20892. vi. The FAR Provision 52.212-1, Instructions to Offerors -- Commercial, applies to this acquisition and is hereby incorporated by reference. All Federal Acquisition Regulations (FAR) clauses may be viewed at http://acquisition.gov/comp/far/index.html. vii. EVALUATION OF PROPOSALS: The Government will evaluate proposals that are determined technically acceptable in accordance with the Technical Evaluation factors. The Government intends to award a single contract from this solicitation. The Government reserves the right not to award a contract. Evaluation of Proposals : The Government reserves the right to make an award without discussions based solely upon initial proposals. EVALUATION FACTORS : 1. Have the ability to screen candidate genes for loss of function neurological phenotypes using Drosophila melanogaster. 2. Have the ability to screen candidate mutations for loss or gain of function neurological phenotypes using Drosophila melanogaster. 3. Have the ability to performed detail phenotypic and behavioral characterization of the mutant fruit flies. 4. Have the ability to provide reports in a timely fashion and record all experimental data in the UDP laboratory electronic notebook. A. Price (This will be Firm-Fixed Price): An evaluation of the offeror's price proposal will be made to determine if proposed prices are realistic for the work to be performed, reflect a clear understanding of the requirements, and are consistent with the technical proposal. Reasonableness determinations will be made by determining if competition exists, by comparing proposed prices with established commercial or GSA price schedules (if applicable), and/or by comparing proposed prices with the Independent Government Cost Estimate (IGCE). viii. In accordance with FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items, offerors must complete annual representations and certifications on-line at https://www.sam.gov/portal/public/SAM/. ix. FAR clause 52.212-4, Contract Terms and Conditions - Commercial Items applies to this Acquisition. x. FAR clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items applies to this Acquisition. xi. The Defense Priorities and Allocations System (DPAS) and assigned rating does not apply to this acquisition. Offerors shall submit their proposals no later than 7:30 a.m. Eastern Standard Time (EST) on July 9, 2013. The proposal must reference the RFP Number: NHLBI-CSB-(HG)-2013-200-DLM. All responsible offerors may submit a proposal, which if timely received, shall be considered by the agency. Proposals can be emailed to the Contracting Specialist, Dorothy Maxwell at maxwelld@nhlbi.nih.gov; Quotations shall not be deemed received by the Government until the quotation is entered into the e-mail address inbox set forth above. Faxed proposals will NOT be accepted. Offerors' proposals shall not be deemed received by the Government until the proposal is entered into the e-mail address inbox set forth above. All quotes shall contain the following: 1. Name of Contracting Organization; 2. Contract Number (for subcontracts, provide the prime contract number and the subcontract number); 3. Contract Type; 4. Total Contract Value and Discounts; 5. Description of Requirement; 6. Contracting Officer's Name and Telephone Number; 7. North American Industry Classification System (NAICS) Code; and 8. DUNS Number. Offerors must complete annual representations and certifications on-line at https://www.sam.gov/portal/public/SAM/.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/HHS-NIH-NHLBI-CSB-(HG)-2013-200-DLM/listing.html)
- Place of Performance
- Address: NIH, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03105809-W 20130704/130702235328-bcb74f9f901eceef41d3cfa615a27890 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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