SOLICITATION NOTICE
Q -- Talen mediated knock-in zebrafish models for Phenotyping
- Notice Date
- 7/15/2013
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541712
— Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHS-NIH-NHLBI-CSB-(HG)-2013-212-DLM
- Archive Date
- 8/2/2013
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- i. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. ii. The solicitation number is NHLBI-CSB-(HG)-2013-212-DLM and the solicitation is being posted as a request for proposal (RFP). Offerors shall submit their proposals no later than 7:30 a.m. Eastern Standard Time (EST) on July 18, 2013. iii. A statement that the solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-68, June 26, 2013. iv. The associated North American Industry Classification System (NAICS) code for this is 541712, Research and Development in the Physical, Engineering, and Life Sciences (except biotechnology) and small business size standard is 500. PURPOSE: The purpose of this requirement is for introducing mutations in zebrafish in order to characterize the potential causality of mutations identified in UDP patients. The Contractor shall provide TALEN mediated knock-in zebrafish models containing specific mutations in order to recapitulate disease phenotype as seen in UDP patients. v. Background : The National Human Genome Research Institute (NHGRI), Undiagnosed Disease Program (UDP) is a trans-National Institute of Health (NIH) initiative that focuses on the most puzzling medical cases referred to the NIH Clinical Center in Bethesda, Md. It was organized by the National Human Genome Research Institute (NHGRI), the NIH Office of Rare Diseases Research (ORDR) and the NIH Clinical Center. Many medical specialties from other NIH research centers and institutes contribute the expertise needed to conduct the program, including endocrinology, immunology, oncology, dermatology, dentistry, cardiology and genetics, among the dozens of participating senior attending physicians. A longstanding medical condition that eludes diagnosis by a referring physician can be considered undiagnosed and may be of interest to this clinical research program. The Undiagnosed Disease Program (UDP) offers patients the hope of a diagnosis and the possibility of therapeutic strategies. In return, patients provide UDP researchers the opportunity to gain new insights about genetic and biochemical mechanisms of disease and insights into normal cell biology, biochemistry and physiology. So far, UDP researchers have encountered patients with uncommon presentations of known disorders, multi-systemic complex disorders and new disorders that have never been diagnosed. UDP clinical researchers are using advances in DNA sequencing to detect defects in genes that point to known disorders. These tools offer the potential for discoveries about the role of molecular and biochemical events that can cause disease and, eventually, the development of diagnostic and therapeutic approaches for rare and common diseases. CONTRACTOR REQUIREMENTS : 1. The Contractor shall model missense mutations implicated in human disease in zebrafish by using a TALEN mediated homologous recombination technology to specifically knock-in mutations or the CRISPR/Cas9 method to facilitate homologous recombination. 2. The Contractor shall provide verification of germline transmission of mutation of interest and homologous combination by methods including both PCR and Southern blot analysis. 3. The Contractor shall provide phenotypic analysis of zebrafish for features of the human disease of interest. 4. The Contractor's turn-around-time for UDP to receive results will be 52 weeks or less per 10 genes of interest. 5. The Contractor shall supply real-time free and full sharing of all data with the UDP and its collaborators. 6. The Contractor shall supply electronic copies of all reports appropriate for deposition in the UDP process management system and for other collaborations. Desired Results/Functions/End Items: 1. Test results with qualified interpretations in a timely way given the requirements of the particular test. 2. Research collaborations will be conducted via Videoconferences, email communications, shared professional presentations, posters and publications. Grand Round presentations at the Contractor's location and the NIH Clinical Center may also occur. Technical Specifications/Standards/Methods : 1. The Contractor shall supply mutant fish sperm or mutant embryos to the UDP for phenotypic analysis. 2. The Contractor shall supply results with qualified interpretations in formats appropriate to the process management systems and for facilitation of other collaborations. Special Handling or Handling Requirements : 1. The Contractor shall provide result reports and record methods and materials as appropriate to the highest scientific standards. These materials, methods and results will be shared will agreed upon UDP collaborators. Government Responsibilities: 1. The Government will provide to the Contractor: 2. The Government will not furnish any government property to the vendor. 3. The Government will provide the names and mutations of the genes of interest to the Contractor. 4. The Government will not furnish any facilities to be used by the vendor. The Government will be responsible for reviewing and approving reports and similar matters generated: 1. Results will be provided electronically to the UDP via secure email, the UDP ftp site or direct deposition into the UDP process management system. 2. Results will also be shared with UDP collaborators as defined mutually. Reporting Requirements and Deliverables: 1. The Government will receive all electronic results and biological materials within 52 weeks of notification for each ten genes of interest. 2. The Government will receive additional reports for any test added-on after the Contractor receives the original specimen. OTHER CONSIDERATIONS: 1. A screen of missense mutations implicated in human diseases in zebrafish. This will be accomplished by using a TALEN mediated homologous recombination technology that allows knock-in of the specific mis-sense mutations or the CRISPR/Cas9 method to facilitate homologous recombination in zebrafish. 2. Germline transmission of the mutation of interest and proven homologous recombination by methods including both PCR and Southern blotting. 3. Proven quantitative phenotypic analyses of zebrafish for features of the human disease of interest. 4. A turn-around-time (TOT) of 52 weeks or less per 10 genes of interest. 5. Real time free and full sharing of all data with the UDP and its collaborators. Recording of data in the UDP laboratory notebook and database. 6. A proven track record of at least 20 years of genetic and transgenic analysis of zebrafish. 7. Co-publication of results with the NIH UDP. 8. The potential to extend the studies to define disease mechanisms and identify potential therapeutic interventions. Anticipated Period of Performance: Twelve (12) months from the date of award. vi. NIH/UDP is located at 9 Memorial Drive, Bethesda, Maryland 20892. vii. The FAR Provision 52.212-1, Instructions to Offerors -- Commercial, applies to this acquisition and is hereby incorporated by reference. All Federal Acquisition Regulations (FAR) clauses may be viewed at http://acquisition.gov/comp/far/index.html. viii. EVALUATION OF PROPOSALS The Government will evaluate proposals that are determined technically acceptable in accordance with the Technical Evaluation factors. The Government intends to award a single contract from this solicitation. The Government reserves the right not to award a contract. Evaluation of Proposals: The Government reserves the right to make an award without discussions based solely upon initial proposals. EVALUATION FACTORS : 1. Ability to provide a TALEN mediated homologous recombination technology to specifically knock-in mutations found in undiagnosed disease genes into the genome of zebrafish. 2. Ability to provide data using CRISPR/Cas9 method to facilitate homologous recombination in collaboration with UCLA and UDP. A. Price (This will be Firm-Fixed Price): An evaluation of the offeror's price proposal will be made to determine if proposed prices are realistic for the work to be performed, reflect a clear understanding of the requirements, and are consistent with the technical proposal. Reasonableness determinations will be made by determining if competition exists, by comparing proposed prices with established commercial or GSA price schedules (if applicable), and/or by comparing proposed prices with the Independent Government Cost Estimate (IGCE). ix. In accordance with FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items, offerors must complete annual representations and certifications on-line at https://www.sam.gov/portal/public/SAM/. x. FAR clause 52.212-4, Contract Terms and Conditions - Commercial Items applies to this Acquisition. xi. FAR clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items applies to this Acquisition. xii. The Defense Priorities and Allocations System (DPAS) and assigned rating does not apply to this acquisition. Offerors shall submit their proposals no later than 7:30 a.m. Eastern Standard Time (EST) on July 18, 2013. The proposal must reference the RFP Number: NHLBI-CSB-(HG)-2013-212-DLM. All responsible offerors may submit a proposal, which if timely received, shall be considered by the agency. Proposals can be emailed to the Contracting Specialist, Dorothy Maxwell at maxwelld@nhlbi.nih.gov; Quotations shall not be deemed received by the Government until the quotation is entered into the e-mail address inbox set forth above. Faxed proposals will NOT be accepted. Offerors' proposals shall not be deemed received by the Government until the proposal is entered into the e-mail address inbox set forth above. All quotes shall contain the following: 1. Name of Contracting Organization; 2. Contract Number (for subcontracts, provide the prime contract number and the subcontract number); 3. Contract Type; 4. Total Contract Value and Discounts; 5. Description of Requirement; 6. Contracting Officer's Name and Telephone Number; 7. North American Industry Classification System (NAICS) Code; and 8. DUNS Number. Offerors must complete annual representations and certifications on-line at https://www.sam.gov/portal/public/SAM/.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/HHS-NIH-NHLBI-CSB-(HG)-2013-212-DLM/listing.html)
- Place of Performance
- Address: NIH, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03116189-W 20130717/130715234500-3d11c6425af6341e4a26980d896577d2 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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