SOLICITATION NOTICE
65 -- Synopses - Notice of Intent to Award Sole Source
- Notice Date
- 8/22/2013
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA, 820 Chandler Street, Frederick, MD 21702-5014
- ZIP Code
- 21702-5014
- Solicitation Number
- 0010363549
- Response Due
- 9/3/2013
- Archive Date
- 10/21/2013
- Point of Contact
- Abraham Pottenger, 301-619-2294
- E-Mail Address
-
US Army Medical Research Acquisition Activity
(abraham.pottenger.civ@mail.mil)
- Small Business Set-Aside
- N/A
- Description
- Synopses - Notice of Intent to Award Sole Source to Qiagen Date: 22 August 2013 Classification Code - 334516 - Analytical Laboratory Instrument Manufacturing (size standard 500) Contracting Office Address - USA Medical Research Acquisition Activity, 820 Chandler Street, Fort Detrick, MD 21702-5014 Subject: Notice of Intent to Sole Source a Contract to Qiagen Inc. to support established protocols dictate the use of Qiagen sample prep and PCR products for comparability with the existing data sets and for accurate translation of previous and future data sets. Closing Response Date: Notice of Intent - 3 September 2013 Contact Point: Abraham Pottenger at abraham.pottenger.civ@mail.mil or 301-619-2294. The United States Army Medical Research Acquisition Activity (USAMRAA), Fort Detrick, MD intends to issue a sole source to Qiagen Inc. 27220 Turnberry Ln, Valencia, CA, 91355-1018 The purchase of the required supplies from Qiagen is vital to continuing ongoing research work at USAMRIID. Qiagen reagents and instrumentation is at the core of sample preparation for DSD and other supported divisions at USAMRIID. Many protocols, SOPs, and collaborators dictate the use of Qiagen reagents for documented and approved standards. Additionally, kits for automated systems are proprietary and do not have alternative for use in Qiagen instrumentation. Qiagen brand reagents and supplies are being purchased per the manufacturer's recommendation, and will be used to maintain the systems in a condition consistent with the terms of the manufacturer's warranty. The use of any other reagents and supplies is not recommended by the manufacturer and would void the manufacturer's warranty of the systems. Finally, previously established protocols dictate the use of Qiagen sample prep and PCR products for comparability with the existing data sets and for accurate translation of previous and future data sets. Other manufacturers' supplies do not include the proprietary technology that is required to optimize the performance of the Qiagen Systems or division standards. Qiagen is the sole manufacturer and provider of the required consumables and reagents. The use of any other supply would result in unacceptable delays and duplication of costs. Diagnostics Systems Division is tasked with supporting sample preparation and PCR analysis of numerous sample types in accordance with standards, protocols, and instrumentation used both internally and by external collaborators. DSD is in need of a wide breadth of sample prep kits which are comparable within the same manufacturing standards/buffers and for use in complimenting automated systems. The following are the Minimal Essential Characteristics for the reagents to be procured for DSD. a.HotStar DNA Polymerase (250U) - Must support highly specific PCR and tolerate room-temperature handling. Must include complimenting PCR buffers and Q-Solution for GC rich DNAs. Must be consistent with established and standardized MassCode PCR protocols. b.dNTP Mix, PCR Grade (200uL) - Must function with PCR and RT-PCR reactions and be of high purity. Must be provided at 100mM concentration. Must be consistent with established and standardized MassCode PCR protocols. c.Multiplex PCR Kit (100 RXNs) - Must be highly specific and sensitive and require no optimization. Must have built-in hot start. Must include Q-Solution. Must be consistent with established and standardized MassCode PCR protocols. d.MinElute PCR Purification Kit (250 RXN) - Must be capable of purifying DNA greater than 70 basepairs using silica-based columns. DNA binding must be at least 5 ug, and the elution volume must be 10-15 ul. Eluted DNA must be compatible for sequencing, ligation reactions, restriction digest, microarray, and PCR and standard tabletop microcentrifuges. e.Rneasy MinElute Cleanup Kit (50) - Must concentrate RNA to 10uL and clean up RNA that has been purified by different methods. Must be able to effectively clean up enzymatic reactions. f.EZ1 Virus Mini Kit v2.0 (48 RXN) - Must be capable of purifying both DNA and RNA from virus using the EZ1 automated extraction robot. System must be compatible with serum, plasma, urine, and other bodily fluids. g.QIAamp DNA Mini Kit (250 RXN) - Must be capable of purifying DNA up to 50 kb from multiple sample types including viral, bacterial, genomic, parasitic samples from tissue samples using a silica-based column. Purified DNA must be compatable with downstream molecular assays including PCR. h.QIAamp Viral RNA Mini Kit (250 RXN) - Must be capable of purifying viral RNA from biological samples including serum and plasma using silica-based columns. Samples must be directly compatible with RT-PCR and standard tabletop microcentrifuges. i.EZ1 DNA Tissue Kit (48 RXN) - Must be capable of purifying genomic DNA from tissue and other samples using the EZ1 automated extraction robot. j.Genomic DNA Buffer Set - Required for use with the Genomic Tip 500/G columns. Buffers required include buffers to lyse bacteria, yeast, blood, tissue, and cells. k.Proteinase K (10 ml at greater than 600 mAU/ml) - Required for use with the Genomic Tip 500/G columns. Reagent must have high activity for protein digestion during DNA and RNA purification. l.Genomic Tip 100/G (25 column) - Required for purifying large, intact DNA up to 150 kb in size for whole genome, next generation sequencing. Tips must be gravity flow and purify high-quality genomic DNA. m.EZ1 DSP Virus Kit (48 RXNs) - Must be compatible with Qiagen EZ1 automated system. Must have high sensitivity for low titer viruses. Must enable standardized processing. n.EZ1 Advanced DSP Virus Card - Must be compatible with Qiagen EZ1 automated system and provide capability to run DSP virus kit protocol. o.QIAquick Gel Extraction Kit (250 RXNs) - Must be capable of purifying DNA from agarose gels using silica-based columns. Columns must bind DNA up to 10 kb and exclude fragments less than 70 base pairs. System must have a high recovery rate. Eluted DNA must be compatible for sequencing, ligation reactions, restriction digest, and PCR and standard tabletop microcentrifuges. p.miRNeasy Mini Kit (50 RXN) - Must purify miRNA and total RNA. Must provide enrichment of miRNA and RNAs less than 200 nucleotides. Must provide high-purity RNA for numerous subsequent applications. q.REPLI-g Midi Kit (100 RXN) - This kit must amplify whole genome DNA from small amounts of starting material ( greater than 10 ng DNA) using Phi29 polymerase. Amplified DNA must be compatible with PCR, next-generation sequencing, and microarray. This announcement constitutes the only notice by the government; a written solicitation will not be issued. All sources that believe they are eligible to provide these items must respond in writing. Responses must be supported with clear and convincing evidence to clearly articulate the ability to provide the items outlined above. A request for documentation will not be considered an affirmative response. This Special Notice of Intent is not a request for competitive quotes. Information received will be considered solely for the purpose of determining whether a competitive procurement is in the best interest of the Government. If no written responses are received by the date listed below, which reflects fifteen (15) days after the publication of this notice, the purchase will be awarded without further remark. All questions and responses concerning this notice shall be emailed to Abraham Pottenger at abraham.pottenger.civ@mail.mil Interested concerns must identify their capability in writing to the above e-mail address or by mail to the address listed below no later than 11:00 am EST, 3 September 2013. Contracting Office Address: US Army Medical Research Acquisition Activity, ATTN: MCMR-AAA-MA 820 Chandler Street, Frederick, MD 21702-5014
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/USA/USAMRAA/DAMD17/0010363549/listing.html)
- Place of Performance
- Address: US Army Medical Research Acquisition Activity ATTN: MCMR-AAA, 820 Chandler Street Frederick MD
- Zip Code: 21702-5014
- Zip Code: 21702-5014
- Record
- SN03158313-W 20130824/130822235409-9925b6abb967a15dec252a96caa6b11a (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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