SOLICITATION NOTICE
66 -- Seahorse XFe96 Extracellular Flux Analyzer.
- Notice Date
- 8/28/2013
- Notice Type
- Presolicitation
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, 6001 Executive Boulevard, Room 4211 - MSC 9559, Bethesda, Maryland, 20892, United States
- ZIP Code
- 20892
- Solicitation Number
- SS3021071
- Point of Contact
- Mario D. Gray, Phone: 3014352238
- E-Mail Address
-
Mario.Gray@nih.hhs.gov
(Mario.Gray@nih.hhs.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT WITHOUT PROVIDING FOR FULL AND OPEN COMPETITION. The National Institute on Drug Abuse (NIDA), Station Support Contracts and Simplified Acquisitions Branch (SS/SA) on behalf of the NIDA intends to negotiate and award a contract without providing for full and open competition to Seahorse Bioscience Incorporated for the Seahorse XFe96 Extracellular Flux Analyzer. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE: The intended procurement is classified under NAICS Code: 334516, with a Size Standard: 500 Employees. REGULATORY AUTHORITY: The resultant contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 2005-69 effective August 1, 2013. This acquisition is conducted under the procedures as prescribed in the FAR Subpart 13.5-Test Program for Certain Commercial Items and the FAR Subpart 12-Acquisition of Commercial Items at an amount exceeding the simplified acquisition threshold ($150,000) and not exceeding $6.5 million. STATUTORY AUTHORITY: This acquisition is conducted under the authority of 41 U.S.C. 253(c)(1) under provisions of the statutory authority of the FAR Subpart 6.302-1 Only one responsible source and no other supplies or services will satisfy agency requirements. DESCRIPTION OF REQUIREMENT: Seahorse XFe96 Extracellular Flux Analyzer: XF Technology: Simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of living cells. Convenient, easy-to-use XFe Stress Test Kits and reagents simplify the study of cellular metabolism by providing pre-calibrated, pre-tested reagents for measuring mitochondrial respiration, glycolysis and fatty acid oxidation in cells. Uses disposable cell culture microplates. Non-invasive measurement requires no addition of dyes, labels or reporters. Cells/microplate remain viable following XF measurement and may be used for another assay. OCR and ECAR rate are calculated in 2-5 minutes using data acquired every 14 seconds. Measurements may be repeated indefinitely to measure kinetic responses. Typically requires only 30,000 to 80,000 cells per 24-well, 12,000 to 30,000 per 96-well assay. No cleaning required. All parts that contact cells, media or drugs are disposable. Measures adherent cells without requiring trypsinization. Suspended cells may be measured using Cell-Tak® cell and tissue attachment medium. Up to four test compounds may be added automatically to each well during the assay. Measurements may be performed before and after each compound is added. Measurement technology is covered by patent # 7,276,351. XFe Analyzer: Compact bench-top instrument 41cm W x 61cm D x 43cm H. High resolution, 19" touch screen controller for high productivity. Temperature controlled measurement chamber is maintained at 37C +/- 5°C. XFe data display shows OCR and ECAR data simultaneously. XFe Wave software provides integrated data analysis, and data is easily exported into MS Excel for transfer into other statistical analysis programs. XFe Wave software is provided with an unlimited-use license for one institution. XFe Assay Kits and Reagents: Standardized reagent kits and protocols designed for use with the XFe Analyzer. Convenient, quality controlled kits provide standardized metabolic measurements in an easy-to-use format. XFe sensor cartridges employ robust, low cost optical sensor technology. Background: The Cellular and Molecular Neurosciences Section (CMNS) of the Laboratory of Neurosciences (LNS) mission is to investigate the cellular and molecular processes underlying neuronal aging, as well as neurodegenerative disorders such as Alzheimer's, Parkinson's, and Huntington's diseases, in order to ameliorate/develop therapeutic strategies against it. Some of the potential therapeutic strategies investigated are dietary manipulations (i.e. caloric restriction, ketogenic diet, every other day feeding, phytochemicals) and exercise. Purpose and Objectives: The Seahorse XFe96 analyzer will allow to determine the impact of the dietary and exercise regiments on brain bioenergetics at the molecular level. Combined with the use of genetically engineered cells and animals we will have the opportunity to elucidate signaling pathways that can be used as therapeutic targets. Several other IRP investigators could also benefit from the acquisition of the Seahorse XFe96 analyzer to further their studies. Period of Performance/Delivery and Contract Type: The delivery will occur within 30 days. The contract type is Firm-Fixed-Price. Project Description: The Seahorse XFe96 analyzer will allow to determine the impact of the dietary and exercise regiments on brain bioenergetics at the molecular level. Combined with the use of genetically engineered cells and animals we will have the opportunity to elucidate signaling pathways that can be used as therapeutic targets. Several other IRP investigators could also benefit from the acquisition of the Seahorse XFe96 analyzer to further their studies. Other Important Considerations: Not Applicable. CONTRACTING WITHOUT PROVIDING FOR FULL AND OPEN COMPETITION: The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted as prescribed in FAR Part 10-Market Research, specifically the review and evaluation of the responses to the sources sought notice published in the FedBizOpps. Only one responsible source exists to sell the needed equipment which will satisfy the Government's requirements. Thus, the needed equipment can perform functions, which cannot be found in any other commercially available equipment. The essential functions of the needed equipment is as follows: Acquisition is for one Seahorse XFe96 analyzer (cat 101991-100) and 5 packages of the consumables (XFe96 FluxPak, cat 102416-100)) required to perform the required assays from Seahorse Bioscience. In order to investigate the impact of dietary manipulations (i.e. caloric restriction, ketogenic diet, every other day feeding, phytochemicals) and exercise on brain bioenergetics in vitro, we require an instrument able to simultaneously measure the mayor metabolic pathways of cells, glycolysis and mitochondrial respiration. The NIH/NIA experimental plans also include using living model organism such as Caenorhabditis elegans which are globally used as a model for physiological and pathological aging research. Given the unique features of the main cell type (neurons) and living organisms will be utilized in our projects, the measurements must be performed in a non invasive way without the need of labeling dyes or other invasive tools (i.e, reporter plasmid transfection, electrodes and so on). The NIH/NIA also need to be able to utilize the samples, either cells or organisms, after the measurements for other biochemical or molecular techniques to dissect the molecular events leading to a specific metabolic response under physiological conditions and in experimental models of age related neurodegenerative disorders. Based on our searches the Seahorse XFe96 analyzer (cat 101991-100) allows to simultaneously measure oxygen consumption rate (OCR-mitochondrial respiration) and extracellular acidification rate (ECAR- glycolysis) of living cells and organisms, in a non invasive way without the need of dyes or reporters, as per our requirements. The instrument also allows the NIH/NIA to reuse the sample after the measurements and, as an added bonus, to perform the tests in a 96 wells format thus simultaneously reducing the number of cells/organism needed for assay and increasing the number of experimental variables can be tested at a given time. CLOSING STATEMENT: This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must reference the identifier code as follows: SS3021071. All responses must be submitted electronically to the Contract Specialist, Mario Gray, using the e-mail address as follows: Mario.Gray@nih.hhs.gov, and, by the closing date and time of this notice. Fax responses will not be accepted.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-01/SS3021071/listing.html)
- Place of Performance
- Address: Baltimore, Maryland, 21224, United States
- Zip Code: 21224
- Zip Code: 21224
- Record
- SN03166497-W 20130830/130829000632-510b2d9fed9e8a150eaa4ec3ecdacdb6 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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