SOLICITATION NOTICE
B -- Follow-on Requirement for Whole Exome Sequencing
- Notice Date
- 12/17/2013
- Notice Type
- Presolicitation
- NAICS
- 541711
— Research and Development in Biotechnology
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- NIH-HHS-NHLBI-CSB-(HG)-2014-038-DLM
- Archive Date
- 1/4/2014
- Point of Contact
- Dorothy Maxwell, Phone: 301-435-0352
- E-Mail Address
-
maxwelld@mail.nih.gov
(maxwelld@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION: The National Institute of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI) Office of Acquisition (OA) on behalf of the National Human Genome Research Institute, (NHGRI), intends to negotiate and award a purchase order on a noncompetitive sole source basis to Axeq Technologies, 9700 Great Seneca Highway, Rockville, Maryland 20850 to procure the following: Procurement: This is a Follow-On Requirement. 1. Exome Sequencing of 100 Samples, 75-fold sequence coverage at the raw data level in pursuit of 30-fold (30X) on target coverage, with 100-bp paired-end sequencing method. 2. Raw data (Fastq) will be provided in hard disk drive. BACKGROUND : The National Institutes of Health (NIH) is the nation's leading medical research agency and the primary Federal agency conducting and supporting medical discoveries that improve people's health and save lives. The National Human Genome Research Institute (NHGRI), National Institutes of Health Undiagnosed Disease Program (UDP) is a trans-National Institute of Health (NIH) initiative that focuses on the most puzzling medical cases referred to the NIH Clinical Center in Bethesda, MD. It was organized by the National Human Genome Research Institute (NHGRI), the NIH Office of Rare Diseases Research (ORDR) and the NIH Clinical Center. Many medical specialties from other NIH research centers and institutes contribute the expertise needed to conduct the program, including endocrinology, immunology, oncology, dermatology, dentistry, cardiology and genetics, among the dozens of participating senior attending physicians. A longstanding medical condition that eludes diagnosis by a referring physician can be considered undiagnosed and may be of interest to this clinical research program. identified new and rare diseases. Objectives: The purpose of this particular sequencing project is to perform clinical grade quality whole exome sequencing on patient and familial DNA. All data must be produced using the same instrumentation and methodology that the UDP has used thus far in order to allow integration of this data with current databases and analysis using in-house software programs. Genomic DNA will be isolated from all admitted Undiagnosed Diseases patients and family members. Depending on the phenotype of the patient, these DNA samples will be sent for exome sequencing as the first step to obtain variant information that may determine the mechanism of the patients' disease. It is necessary for the Undiagnosed Diseases Laboratory to perform analyses on the exome variant data in a timely manner to facilitate genetic studies when combined with SNP analysis. This information will then be used to perform research experiments to discern the genetic and molecular basis of the unique disease of the patient Period of Performance : The contractor will provide 100 DNA samples for Exome sequencing. Sequencing data will be provided on a hard disk drive to the NIH Undiagnosed Diseases Program in Fastq and BAM forms within 6-8 weeks. JUSTIFICATION: Axeq Technologies provides exome sequencing data using the Illumina HiSeq 2000 or 2500 Sequencer and offer a minimum of 30X on target coverage with 100 bp paired-end sequencing method. Axeq will continue to provide raw data in the form of Fastq and BAM for the DIR-NHGRI-Undiagnosed Diseases program (UDP) on a hard disk drive within 8 weeks of submission. The sequencing instrumentation parameters, chemistry, and report data format must remain consistent with the results previously obtained in order to perform WES filtering and analyses with SNP data using programs developed in-house and to allow for direct comparison for ongoing research. Contractor Requirements : • Exome sequencing of 100 purified genomic DNA samples from the NIH Undiagnosed Diseases Program, without the need for phenol/chloroform extraction • Exome sequencing must be performed using the Illumina HiSeq 2000 or 2500 and related instrumentation and software • Enrichment must be performed using the Illumina TruSeq Enrichment Kit or the Nextra 62 Mb Extended Enrichment kit. • Samples must be sequenced to a least 30-fold (30X) on target coverage with 100 bp paired-end sequencing method • Turnaround time must be 8 weeks or less including sample QC • Raw data in the form of Fastq and BAM will be provided in a hard disk drive to the NIH UDP • The contractor must pay for shipment of the samples to the sequencing laboratory • The contractor must provide real time access to the status of the project, download reports and data immediately after they are available • The contractor must provide managerial support for the project 24/7 Government Responsibilities : a. The government will not furnish any government property to the vendor. b. The government will provide 100 purified genomic DNA samples to the vendor. c. The government will not furnish any facilities to be used by the vendor. Reporting Requirements and Deliverables : 1. The Government will receive real time access to the status of the project, download reports and data immediately after they are available. 2. The Government will receive at least 75-fold average sequencing coverage at the raw data level with 100 bp paired-end sequencing method. 3. The Government will receive raw sequencing data in the form of Fastq and BAM on a hard disk drive within 8 weeks of DNA submission. Inspection and Acceptance Requirements : 1. The Government will inspect Clinical grade quality sequencing data with 75-fold average coverage at the raw data level with 100bp paired-end sequencing method will be in the form of Fastq and BAM files located on a hard disk drive will be accepted from the Contractor. The Contractor shall be contacted via phone or email upon delivery, if any problems are discovered. 2. The Contractor shall provide Clinical grade quality sequencing data on a hard disk drive to the NIH Undiagnosed Diseases Program in Fastq and BAM forms within 8 weeks of submission. 3. A Government Official will inspect and accept requirements. REGULATORY AUTHORITY: This acquisition is conducted under the authority of the Federal Acquisition Regulations (FAR) Subpart 13.106-1(b) Soliciting from a single source, only one responsible source and no other supplies or services will satisfy agency requirements. ADDITIONAL INFORMATION: Industry Classification (NAICS) Code is 541711, Research and Development in Biotechnology, with a business size standard of 500. The acquisition is being conducted under FAR Part 13, simplified acquisition procedures, therefore the requirements of FAR Part 6 B Competitive Requirements are not applicable (FAR Part 6.001). The resultant Contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 05-69 (September 3, 2013). This requirement is under the SAT of $150,000.00. This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received by December 20, 2013 and must reference synopsis number HHS-NIH- NHLBI-CSB-(HG)-2014-038-DLM, may be submitted to the National, Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6149, Bethesda, Maryland 20892-7902, Attention: Dorothy Maxwell. Response may be submitted electronically to maxwelld@mail.nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. "All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/NIH-HHS-NHLBI-CSB-(HG)-2014-038-DLM/listing.html)
- Place of Performance
- Address: Vendor's Location, United States
- Record
- SN03253668-W 20131219/131217235627-b66f566f4b7bd8a2db1343da9f810bf4 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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