SOLICITATION NOTICE
J -- Maintenance Agreement for the PE Enspire Multimodal Plate Reader
- Notice Date
- 5/19/2014
- Notice Type
- Presolicitation
- NAICS
- 811219
— Other Electronic and Precision Equipment Repair and Maintenance
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Rockville, Maryland, 20852, United States
- ZIP Code
- 20852
- Solicitation Number
- N02RC42534-83
- Archive Date
- 6/18/2014
- Point of Contact
- Gwennifer K. Epps, Phone: 240-276-5445, Terry Galloway, Phone: 240-276-5384
- E-Mail Address
-
eppsg@mail.nih.gov, gallowaytl@mail.nih.gov
(eppsg@mail.nih.gov, gallowaytl@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Urologic Oncology Branch (UOB), plans to procure on a sole source basis a service contract for a PE Enspire Multimodal Plate Reader, from PerkinElmer Health Sciences Inc.,710 Bridgeport Avenue, Shelton, CT 06484-4794, United States. This acquisition will be processed in accordance with simplified acquisitions procedures as stated in FAR Part 13 13.106-1(b)(1) and is exempt for the requirements of FAR Part 6. The North American Industry Classification System code is 811219, and the business size standard is $19.0 Million. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. It has been determined there are no opportunities to acquire green products or services for this procurement. The period of performance shall be for twelve months from the date delivery and installment. The Urologic Oncology Branch, (UOB) conducts clinical and basic research designed to develop better methods for detection, prevention, and therapy of patients with genitourinary malignancies. The primary focus of the Urologic Oncology Branch is the study of the genes associated with initiation and progression of kidney and prostate cancers, and to do translational research via clinical trials. UOB has a clinical surgery tumor sample bank, tumor cell lines, patient blood or other samples in a branch repository. To reach its objective, NCI/UOB has started a promising collaboration with National Center for Advancing Translational Sciences (NCATS) to screen small molecules inhibitors and performed combination matrix screens against several types of kidney cancers as well as bladder cancer. A sole source to PerkinElmer Health Sciences Inc. is intended for this order; based on the fact t he PE Enspire Multimodal Plate Reader utilizes technology that as unique technology measures the depth (optical pathlength) of samples in a microplate, which, when used with Enspire Software, can automatically normalize the well absorbance to a cuvette equivalent pathlength of 1 cm. This technology is only to PE Devices. These hardware and software capabilities are unique characteristics of the instrument and are not available elsewhere. In addition, the EnSpire is the only plate reader that has a dedicated field-based team (Service, Application Scientists) to support the PerkinElmer Technologies including Label-Free, Luminescence, Alpha, LANCE, and DELFIA. Also, to ensure the function of this equipment, Warranty information must be supplied, and shipping must be quoted. The acquisition of PE Enspire Multimodal Plate Reader will allow UOB scientists to perform a broad range of assays, such as low throughput screens or functional assays, necessary to validate the data obtained from NCI's collaboration with NCATS. Thus the acquisition of this machine is critical to identify in a timely manner to validate lead therapeutic compounds for clinical trials. This will be the first analyzer acquired by NCI's/UOB and used as high throughput for the cancer metabolism initiative and small molecular targeting at Genitourinary Cancers. Note: The Enspire Multimodal Plate Reader must be able to perform the following tasks: (1) protein estimation analysis; (2) enzyme kinetic assays; (3) Competition binding experiments; (4) Viability cell death dual florescent assays; (5) Cytotoxicity assays; (6) Drug screening/ Drug binding assays/FRET; (7) NADPH/NADP and NADH/NAD detection assays; (8) Metabolic assays (glucose uptake, lactate secretion, ATP assays; (9) Colorimetric ROS measurement assays; (10) ELISAs/EIAs, multiple caspase activation assays; (11) Immunoassays, functional Kinase assay (Ca release, cAMP and cGMP measurement, B-arrestine assays); (12) Drug dissolution profiles, and (13) Enzyme kinetics ( e.g., Ki, Km, etc.). In addition: (14) The system must be able to rapidly analyze multiple plates at a time, accurately incubate plates at either ambient temperatures or at temperatures ranging from 4C to 45C. The system would need to have both UV and visible spectrum capabilities and to be able to easily switch between 96- and 384-well formats. The system must be equipped a full size touch screen software interface. (15) The system must be equipped with Quad Monochromators including bar coded excitation and emission, 2 nd order blocking filters for fully automatic identification for positive ID of location and measurement configuration. (16) The system must be equipped with a built in PC to prevent potential theft of desktop and / or laptop PC (17) The system must be equipped with a high performance Quad Monochromator Technology with 5nm band with on both excitation and emission functions, adjustable in 0.1nm increments, thus allowing for maximum precision. (18) The system must include the ability to perform IC50/EC50, linear regression, spline, 4PL/5PL curve fitting capabilities for curve fitting, concentration calculations and flexible report formatting and automatic data export. (19) The system must include shaker with three modes: linear, orbital and double orbital. (20) The system must have plate height sensor, positive identification for filters and mirrors. (21) The system must have plate ID Barcode readers: all four sides of the plate. (22) The system must include a measurement chamber with temperature control plates which enable the system to maintain ambient temperature, minimizing the inconsistency of results caused by temperature difference and evaporation. Temperature Control must be flexible and adjustable from ambient up to 65 degrees Celsius. (23) The system must be capable of being field-upgradeable for all Measurement Technologies post sale except for Temperature Control and Top/Bottom Reading. Temperature Control and Top/Bottom Reading must be specified at point of sale. Additional Salient Characteristics include: a. The system must be easily integrated into a full robotic system and easily adaptable to fit sample processing requirements. b.The system must be able to measure the depth (optical pathlength) of samples in a microplate. c. The sytem's software must automatically normalize the well absorbance to a cuvette equivalent pathlength of 1 cm, equivalent to having 96 or 384 cuvettes. d. The system must allow for the elimination of standard curves, using a known extinction coefficient to calculate directly from absorbance. e. The system must be able to account for pipetting error. f. The system must be able to accommodate 50-300 μL of sample or standard into each well of a 96-well plate (20 μL to 100 μL in a 384-well plate) and read. g. The system must correct for the volume differences in all 96 or 384 wells and report 1 cm absorbance values, and to be able to extend the dynamic range to 6+ OD. h. The system must also be able to quickly and easily test multi-channel dispensers and pipettors by measuring the depth of the liquid in the well in a matter of minutes. Essentially, all UV/VIS spectrophotometric assays that are read in a conventional spectrophotometer or microplate reader. Preventative Maintenance: The contractor shall perform two (2) preventive maintenance inspections during the contract period. Technically qualified factory-trained personnel shall perform Service. Service shall consist of a thorough cleaning, calibration, adjusting, inspection, lubrication and testing of all equipment in accordance with the manufacturer's latest established service procedures. All equipment shall be operationally tested through at least one (1) complete operating cycle at the end of the preventive maintenance inspection to assure optimum and efficient performance. Emergency Service: Emergency repair services shall be provided on an unlimited basis during the term of this contract at no additional cost to the government. Unlimited emergency service shall be provided during normal working hours, Monday through Friday excluding PerkinElmer holidays. Upon receipt of notice that any part of the equipment is not functioning properly, the PE service shall respond to the request within twenty-four (24) hours, furnish a qualified factory-trained service representative to inspect the equipment and perform all repairs and adjustments necessary to restore the equipment to normal and efficient operating condition. Emergency service calls shall not replace the necessity for scheduled PMIs. With unlimited telephone support for instruments, reagents and applications will be provided at no additional charge. This is not a solicitation for competitive quotations. However, if any interested party especially small business, believes they can meet the above requirement, they may submit a statement of capabilities. All information furnished must be in writing and must contain sufficient detail to allow the NCI to determine if it can meet the above unique specifications described herein. An original and one copy of the capability statement must be received in the NCI Office of Acquisitions on or before 11:00 AM EST on, June 3, 2014. No electronic capability statements will be accepted (i.e. email or fax); an original and one copy must be sent to the NCI Office of Acquisitions at the address stated above. All questions must be in writing and can be faxed (240) 276-5401 or emailed to Gwennifer Epps, Contracting Officer at eppsg@mail.nih.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. In order to receive an award, contractors must have valid registration and certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA), through sam.gov. No collect calls will be accepted. Please reference solicitation number N02RC42534-83 on all correspondence.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/N02RC42534-83/listing.html)
- Place of Performance
- Address: Urologic Oncology Branch, Building 10, NIH/National Cancer Institute, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03369560-W 20140521/140519234155-176b9941308a58d413ca69a01d09e10f (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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