SPECIAL NOTICE
66 -- HPM100 High Pressure Freezer
- Notice Date
- 6/23/2014
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, Office of Research Facilities, 9000 Rockville Pike, Bldg. 13, Rm. 2E47, MSC 5738, Bethesda, Maryland, 20892-5738, United States
- ZIP Code
- 20892-5738
- Solicitation Number
- NIH-OLAO-OD3-NOI3409798
- Archive Date
- 7/17/2014
- Point of Contact
- Shirlene M. Smith, Phone: 3014437979
- E-Mail Address
-
ssmith@od.nih.gov
(ssmith@od.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This is a synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 and FAR Part 13, Simplified Acquisitions Procedures. This announcement constitutes the only solicitation; quotations are not being requested and a written solicitation will not be issued. This is a Notice of Intent. The announcement number for this acquisition is NIH-OLAO-OD3-NOI3409798 and is being issued under the authority of FAR Subpart 6.302-1, 10 U.S.C. 2304(c)(1). Only one responsible source and no other supplies or services will satisfy agency requirements. The anticipated award date July 7, 2014. The incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-72 dated January 30, 2014. This acquisition will be processed under Simplified Acquisition Procedures (SAP) and is not a small business set-aside. The associated North American Industry Classification System (NAICS) Code is 334516, which has a size standard of 500 employees. The National Institutes of Health, Office of the Director, Office of Logistics and Acquisitions Operations, on the behalf of the National Institutes Deafness Communication Disorders, intends to procure on a sole source basis from Leica Microsystems Inc., a Leica EM HPM100 High Pressure Freezing Machine 115VAC/60Hz and LN2 Connection Tube L= 1.0m. The EM HPM100 High Pressure Freezing Machine must have the following capabilities: 1)High pressure liquid nitrogen system for excellent preservation of ultra structure; 2)No intracellular anti-freezing agents required; 3)No Leidenfrost phenomenon; 4)p/T correlation system is used to prevent pre-cooling and pressure artefacts; 5)The thermally insulated process chamber has cooling rates upt o 80'000K/s at specimen surface; 6)Universal thin walled specimen carriers are used for easy handling and improved cooling rates throughout the specimen; 7)Application specific specimen carriers are perfect for freezing specimens up to 5 mm in diameter; 8)Fully automated process with specimen transfer into LN2 guarantees reproducible and reliable freezing; 9)Easy one button operation; 10)Space saving integrated workstation includes a stereo microscope with LED; 11)Display of p/T curves and complete data storage on USB stick helps to evaluate sample quality before and post processing; 12)Integrated Liquid Nitrogen Dewar with drain for low LN2 consumption; 13)Oil-free air pressure intensifier; 14)State of the art, compact and mobile low noise design with integrated air compressor; 15)Single phase universal mains; 16)Tool set included; 17)Accessory Kit which included; 18)1x USB Stick; 19)1x middle plate for 3mm sample carriers; 20)2x cartridge cylinders for 3mm sample carriers; 21)10x 3mm sample carriers Type A; 22)10x 3mm sample carriers Type B; 23)1x Cryo forceps with straight tip; 24)1x Forceps with insulation coating; 25)1x 20cm forceps; 26)1x 4 liter Dewar; 27)1x ladle for the sample release Dewar; 28)1x sample carrier release device with 3mm insert and sample holder with six positions; 29)Operating Manual. The LN2 Connection Tube must be able to connect the external dewar to the EM HPM100/EM BAF060 storage dewar. The Laboratory of Cell Structure and Dynamics intends to use the state of art electron microscopy of biological tissue which has been moving into using cryogenically frozen samples. Cryo-samples provide many benefits important for understanding cell/structural biology. The samples have far less processing artifacts found in conventional room temperature preparation that occur from fixation of the samples with aldehydes, dehydration, and embedding in resin. High pressure freezing solves this thickness issure, allowing samples of up to 300um thick to be frozen without ice crystal formation. Freezing auditory tissue requires that the entire depth of the tissue be vitreous ice so that the entire structure can be determined without artifacts. The Leica HPM100 is the state of the art high pressure freezer. Most other high pressure freezers are still essentially based on the original Balzer HPM-010 developed in the 80's. The Leica HPM100 is a true second-generation high pressure freezer with many advancements not available in any other high pressure freezer or by any other freezing technique. Pressure generation and freezing occur simultaneously, which can freeze a sample up to 6mm wide. Freezing of entire nematode worms has even been demonstrated. The HPM100 allows for freezing of samples using a variety of holders including holders designed for freeze substitution, direct cryosectioning, correlative light/electron microscopy, and freeze fracture/etch. Our lab can utilize nearly all of the functions of the HPM100. For the first time, it will be possible to freeze entire organ of Corti and utricle samples from mice and rats. The HPM100 will allow many novel experiments to be performed. This is a fixed-price contract. This notice of intent is not a request for competitive quotations; however, the Government will consider responses submitted. Responses must be submitted no later than 10:00 AM Eastern Standard Time (EST), July 2, 2014 to Ms. Shirlene Smith via e-mail to ssmith@od.nih.gov. Emailed submission ONLY will be accepted. All information furnished must contain sufficient detail to allow the government to determine if it can meet the above specifications described herein. A determination by the Government not to compete the proposed acquisition based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Please reference the announcement number NIH-OLAO-OD3-NOI3409798 on all correspondence. Requests for information concerning this requirement must be submitted in writing can be E-mailed to ssmith@od.nih.gov. It is the vendor's responsibility to confirm receipt of all quotations and/or questions by the closing date of this announcement via e-mail. Calls will not be accepted.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/OLAO/NIH-OLAO-OD3-NOI3409798/listing.html)
- Place of Performance
- Address: National Institute of Health/National Institute Deafness and Other Communication Disorders/, Laboratory of Cell Structure and Dynamics, Porter Neuroscience Research Center, 35 Convent Drive, Building 35A, Room 3D 908A, Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03403165-W 20140625/140623234727-195bb41d61b8bb456def4231bd7fa22e (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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