SOLICITATION NOTICE
R -- Ultra-high throughput deep sequencing analysis of RNA and ChIP samples from immunosenescence and inflammation associated with aging
- Notice Date
- 8/12/2014
- Notice Type
- Combined Synopsis/Solicitation
- NAICS
- 541990
— All Other Professional, Scientific, and Technical Services
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-CSS-14-533
- Archive Date
- 9/2/2014
- Point of Contact
- Hunter A. Tjugum, Phone: 301 435 8780, ,
- E-Mail Address
-
hunter.tjugum@nih.gov, NIDASSSAPurchaseRequ@mail.nih.gov
(hunter.tjugum@nih.gov, NIDASSSAPurchaseRequ@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- This acquisition is being conducted under Federal Acquisition Regulations (FAR) Part 13 - Simplified Acquisition Procedures, and the resultant contract award will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2005-76, dated 25 JULY 2014. The solicitation number is HHS-NIH-NIDA-SSSA-CSS-14-533 and the solicitation is issued as a Request For Quotation (RFQ). The associated NAICS code is 541990 All Other Professional, Scientific, and Technical Services with associated small business size standard $14.0 million. This requirement is released on an unrestricted basis. PROJECT DESCRIPTION Background: The National Institute on Aging (NIA), one of the 27 Institutes and Centers of NIH, leads a broad scientific effort to understand the nature of aging and to extend the healthy, active years of life. The Laboratory of Molecular Biology and Immunology (LMBI) has as its primary goal to elucidate the molecular mechanisms of processes that contribute to age-associated changes in physiology, including diseases whose prevalence increases with age. LMBI research can be viewed in terms of three interconnected themes: 1) Molecular mechanisms of basic cellular processes, 2) Immune cell function, and 3) translating basic mechanisms to clinical research. The Baltimore Longitudinal Study of Aging (BLSA) at the NIA, Intramural Research Program (IRP), studies age-related changes in medical, physiological, cognitive and physical functioning across the adult life span. BLSA participants come to the BLSA on a regular schedule for testing designed to predict normal as well as pathological aging. The Laboratory of Behavioral Neuroscience (LBN) studies age-related changes, including development of a variety of medical conditions and the effects of these comorbidities on physical and psychological health and resilience. Data from the BLSA is used by many of the NIA laboratories in the preparation of analyses, presentations and manuscripts. Purpose and Objectives: The contractor shall provide all resources necessary to accomplish the tasks and deliverables described in this Statement of Work (SOW). The LMBI has a requirement for contractor support to provide ultra-high deep sequencing of RNA and DNA samples including chromatin immunoprecipiptation (ChIP) and input DNA obtained from primary human peripheral blood cell subsets. The objective is to analyze deep sequencing techniques from a powerful ultra-high throughput deep sequencing system to identify low-abundance inter-genic non-coding RNA's in primary human peripheral blood cell subsets. CONTRACTOR REQUIREMENTS: Independently and not as an agent of the Government, the Contractor shall be required to furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government, as needed to perform the tasks as specified herein. Specifically, the contractor shall provide or perform the following: Ultra-high throughput sequencing on a system that can pool samples to be run in a single read cycle sequencing lane to achieve a large number of reads per sample. Barcode library preparation from samples of RNA, DNA, and chromatin immunoprecipiptation (ChIP) and input DNA. The essential government feature is the analysis of samples in a powerful ultra-high throughput sequencing system that enables large-scale genomics plus a broad range of other applications and study sizes, and to deliver RNA and ChIP library preparation as described. Project requirements will include examining age-associated changes in gene expression and epigenetic state of 10 cell subsets purified from human peripheral blood. Cytapheresis packs will be collected by a combination of magnetic bead purification and flow cytometry. These cells will be used to isolate RNA and implement out ChiP. GOVERNMENT RESPONSIBILITIES: The Contracting Officer's Representative (COR) will be responsible for: (1) monitoring the Contractor's technical progress, including the surveillance and assessment of performance and recommending to the Contracting Officer changes in requirements; (2) interpreting the statement of work and any other technical performance requirements; (3) performing technical evaluation as required; (4) performing technical inspections and acceptances required by this contract; and (5) assisting in the resolution of technical problems encountered during performance. INSTRUCTIONS TO OFFERORS Offerors may respond with a proposal indicating their ability to provide the technical requirements stated in this solicitation. Pricing proposals shall indicate the unit price analyze samples described in this announcement on a powerful ultra-high throughput deep sequencing system capable of achieving the scale described in this announcement. Responses from Offerors will be evaluated on showing an Understanding of the Requirements. The Offeror's technical approach shall indicate compliance with Contractor Requirements, and describe an essential understanding of the requirements involved in accomplishing the work. Offerors will be evaluated on their ability to meet the essential government requirements listed in this solicitation. Offerors will be evaluated on past performance for work on government and commercial contracts for similar requirements in the past two (2) years. Responses to this RFQ must not exceed 15 pages in length and be submitted in Microsoft Word or searchable PDF format in a font size of 11 or 12 point and font type of Arial or Times New Romans. Margins must be 1-inch wide (top, bottom, left, and right). Responses must references this solicitation number and include the name of the contractor. Pages in excess of the specified page limit will not be considered or evaluated. The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. In addition the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All Offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." All responses must be received no later than the response deadline indicated on August 18, 2014, 12:00 pm Eastern time. Late responses will not be accepted. All responses must reference solicitation number HHS-NIH-NIDA-SSSA-CSS-14-533. Responses may be submitted electronically to Hunter.Tjugum@nih.gov and to NIDASSSAPurchaseRequ@mail.nih.gov. Fax responses will be accepted at (301) 480-1358. For information regarding this solicitation, contact Hunter Tjugum by email at hunter.tjugum@nih.gov or by phone at (301) 435-8780.
- Web Link
-
FBO.gov Permalink
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- Record
- SN03461602-W 20140814/140813022141-90df29f187b712fe2e69cee364b92734 (fbodaily.com)
- Source
-
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