SPECIAL NOTICE
A -- TECHNOLOGY/BUSINESS OPPORTUNITY FAST PCR INSTRUMENT
- Notice Date
- 8/18/2014
- Notice Type
- Special Notice
- NAICS
- 238990
— All Other Specialty Trade Contractors
- Contracting Office
- Department of Energy, Lawrence Livermore National Laboratory (DOE Contractor), Industrial Partnerships & Commercialization, 7000 East Avenue, L-795, Livermore, California, 94550
- ZIP Code
- 94550
- Solicitation Number
- FBO290-14
- Archive Date
- 9/19/2014
- Point of Contact
- Connie L Pitcock, Phone: 925-422-1072
- E-Mail Address
-
pitcock1@llnl.gov
(pitcock1@llnl.gov)
- Small Business Set-Aside
- N/A
- Description
- TECHNOLOGY/BUSINESS OPPORTUNITY FAST PCR INSTRUMENT Opportunity : Lawrence Livermore National Laboratory (LLNL), operated by the Lawrence Livermore National Security (LLNS), LLC under contract with the U.S. Department of Energy (DOE), is offering the opportunity to license intellectual property for a new Fast PCR Instrument technology for commercialization. Background : RT-PCR is the "gold standard" for fast and efficient nucleic acid analysis and detection. Current benchtop and robotic-based PCR systems are limited in their ability to become the next generation of handheld devices due to their size, speed, complexity and reliance on heating technologies with much less precision and accuracy. This system has the potential to compete on performance, speed, portability, cost and simplicity. Description : The invention is a microfluidic PCR thermal cycling method capable of extremely fast cycles, and a resulting extremely fast detection time. The technology utilizes a robust, stable and precise temperature control system to cycle the DNA microchip at heating and cooling rates greater than 45 ° C per second without overshoot or undershoot. The architecture is both compact, and allows for optical interrogation, resulting in a field-portable, extremely fast real-time PCR system. The technology is described in the publication, " Under-three minute PCR: Probing the limits of fast amplification " (The Analyst, 2011, 136, 3707-3712). It is estimated that with this method, target nucleic acid templates approaching 1 Kb in length can be amplified under five minutes. These technologies are currently being prototyped at LLNL. Advantages : Reducing the time required for pathogen characterization and detection is critical for clinical diagnoses. There is an urgent need for fieldable, high speed, high throughput, pathogen-specific detection. Some advantages include: revolutionary processing speed, simplified control system architecture, low required power, light weight assembly, and significant multiplexing capability across the pathogen spectrum. • Complete integration on a microfluidic platform • Fast thermocycling • Chip can operate in continuous flow, for the highest throughput possible • Potential for portability and miniaturization • The system could be paired with other technologies such as rapid sample preparation, as well as post-PCR analyses such as: capillary gel electrophoresis, mass spectrometry, or sequencing Potential Applications : This technology allows extremely rapid thermal cycling, which will enable PCR analysis times to decrease from approximately 1 hour to sub-5 minutes. The change will enable whole new assays and applications. The simplicity of the heat transfer arrangement provides precise and accurate thermal control superior to current technologies, and eliminates the non-uniform temperature distributions described in the literature for commercial thermal cyclers. Development Status: U.S. Patent 8,720,209, entitled Solid-State Distributed Node-Based Rapid Thermal Cycler for Extremely Fast Nucleic Acid Amplification U.S. Patent Application 12/270,348, entitled Rapid Microfluidic Thermal Cycler for Nucleic Acid Amplification U.S. Patent Application 13/267,767, entitled Methods and compositions for Rapid Thermal Cycling U.S. Patent Application 14/313,790, entitled Porous Media Enhanced Heat Transfer for Injection Molding and Methods for Manufacturing Same Wheeler, E.K., et al., 2011, "Under-three minute PCR: Probing the limits of fast amplification" Analyst, 136, 3707-3712. Majoob, S; et al., 2008, "Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification" International Journal of Heat and Mass Transfer, 51 (9-10), 2109-2122. LLNL is seeking industry partners with a demonstrated ability to bring such inventions to the market. Moving critical technology beyond the Laboratory to the commercial world helps our licensees gain a competitive edge in the marketplace. All licensing activities are conducted under policies relating to the strict nondisclosure of company proprietary information. Please visit the IPO website at https://ipo.llnl.gov/?q=resources-industry-working_with_us for more information on working with LLNL and the industrial partnering and technology transfer process. Note: THIS IS NOT A PROCUREMENT. Companies interested in commercializing LLNL's Fast PCR Instrument technology should provide a written statement of interest, which includes the following: 1. Company Name and address. 2. The name, address, and telephone number of a point of contact. •3. A description of corporate expertise and facilities relevant to commercializing this technology. Written responses should be directed to: Lawrence Livermore National Laboratory Industrial Partnerships Office P.O. Box 808, L-795 Livermore, CA 94551-0808 Attention: FBO 290-14 Please provide your written statement within thirty (30) days from the date this announcement is published to ensure consideration of your interest in LLNL's Fast PCR Instrument technology.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/DOE/LLNL/LL/FBO290-14/listing.html)
- Record
- SN03469125-W 20140820/140818235549-886e322703265d26a88092fb9123be1c (fbodaily.com)
- Source
-
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