SOLICITATION NOTICE
R -- Role of the BDNF Receptor TrkB.T1 in Mammalian Cardiac Function
- Notice Date
- 9/4/2014
- Notice Type
- Presolicitation
- NAICS
- 541712
— Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Rockville, Maryland, 20852, United States
- ZIP Code
- 20852
- Solicitation Number
- N02RC42653-24
- Archive Date
- 10/2/2014
- Point of Contact
- Kimesha Leake, Phone: 2402765669, Terry Galloway, Phone: 240-276-5384
- E-Mail Address
-
kimesha.leake@nih.gov, gallowaytl@mail.nih.gov
(kimesha.leake@nih.gov, gallowaytl@mail.nih.gov)
- Small Business Set-Aside
- N/A
- Description
- The National Cancer Institute (NCI), Center for Cancer Research (CCR), Mouse Cancer Genetics Program (MCGP), plans to procure on a sole source basis services to dissect TrkB.T1 signaling in the heart and to determine by electrophysiological and imaging means if cardiomyocytes are the only cell type responsible for the development of a cardiac phenotype from Gianluca Fulgenzi, Polo Murri, Torrette di Ancona, Via Tronto 10, 60020 Ancona Italy. This acquisition will be processed in accordance with simplified acquisition procedures as stated in FAR Part 13.106-1(b)(1) and is exempt from the requirements of FAR Part 6. The North American Industry Classification System code is 541712 and the business size standard is 500 employees. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. The period of performance is twelve (12) months from date of award. It has been determined there are no opportunities to acquire green products or services for this procurement. The Mouse Cancer Genetics Program (MCGP) was established by Dr. Neal Copeland in 1999 and is currently directed by Dr. Lino Tessarollo. Dr. Shyam Sharan is Deputy Director. Members of the MCGP use molecular mouse genetics as a primary tool to better understand the fundamental processes underlying mammalian development and disease. Currently, the MCGP consists of 11 investigators. Major areas of focus include developmental mechanisms and pathways usurped in cancer, stem cell and cancer-propagating cell function, genome integrity control, tumor suppressor function, transcriptional and epigenetic regulation, hematopoiesis, neurotrophin-activated signaling pathways, angiogenesis and the role of selenium in cancer prevention. The neurotrophin receptor TrkB is essential for normal development and function of the mammalian brain. It is expressed in two major splice variants. A full-length TrkB receptor (TrkB.FL) with an intracellular tyrosine kinase domain that is believed to mediate the crucial effects of brain derived neurotrophic factor (BDNF); and a truncated receptor (TrkB.Tl) lacking tyrosine kinase activity that have only recently been reported to elicit intracellular signaling. While the function of the TrkB.FL has been widely investigated the biological significance of TrkB.T1 is still completely unknown. At the NCI, Dr. Tessarollo's laboratory has found that TrkB.Tl receptors are expressed in cardiomyocytes without the kinase active receptors providing a suitable system to test the functionality of TrkB.Tl in vivo. The Tessarollo lab has also found that systemic TrkB.T1 deletion causes cardiac defects. The overall purpose of the project is to dissect TrkB.T1 signaling in the heart and to determine by electrophysiological and imaging means if cardiomyocytes are the only cell type responsible for the development of a cardiac phenotype. The proposed contractor provides a unique set of skills that are not available through any other source. The contractor is an expert in electrophysiology and in addition, has extensive experience with the analysis of the mdx mouse model, another mutant with cardiac defects. Moreover, the contractor has knowledge in cardiomyocytes isolation and biochemistry. This plethora of expertise will be key to the success of this project. For the completion of the second phase of the project the contractor will be repairing and activating a unique instrument that he has previously built incorporating a photodiode with computer driven shutters and mirrors to measure calcium flux within single cells. The contractor will also be writing and compiling software applications to collect and analyze data acquired through this photodiode system. In addition, the contractor's expertise in the patch clamp and single cell recording apparatus is invaluable. The technical expertise required to use this instrumentation can only be acquired with years of use and experience. The patch clamp system is extremely sensitive and an inexperienced user could easily misinterpret and misconstrue results. This notice is not a request for competitive quotation. However, if any interested party, especially small businesses, believes it can meet the above requirement, it may submit a capability statement, proposal, or quotation, which shall be considered by the agency. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Responses must be received in the contracting office by 12:00 PM EST, on September 17, 2014. All responses and questions must be in writing and faxed 240-276-5401 or emailed to Kimesha Leake, Contract Specialist via electronic mail at Kimesha.leake@nih.gov. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the System for Award Management (SAM) through sam.gov. Reference: N02RC42653-24 on all correspondence.
- Web Link
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- Record
- SN03496715-W 20140906/140905000356-7939bd2b575f707dc159f8dc2203fbbf (fbodaily.com)
- Source
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