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FBO DAILY - FEDBIZOPPS ISSUE OF SEPTEMBER 12, 2014 FBO #4675
SOLICITATION NOTICE

66 -- Brand Name Azure Biosystems c500 Gel/blot Imaging System - (Draft)

Notice Date

9/10/2014
 

Notice Type

Combined Synopsis/Solicitation
 

NAICS

334516 — Analytical Laboratory Instrument Manufacturing
 

Contracting Office

Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E128, Rockville, Maryland, 20852, United States
 

ZIP Code

20852
 

Solicitation Number

N02RC42643-61
 

Archive Date

9/30/2014
 

Point of Contact

KATHY D. ELLIOTT, Phone: 240-276-5570, Seena Ninan, Phone: 240-276-5419
 

E-Mail Address

ELLIOTTK@MAIL.NIH.GOV, ninans@mail.nih.gov
(ELLIOTTK@MAIL.NIH.GOV, ninans@mail.nih.gov)
 

Small Business Set-Aside

Total Small Business
 

Description

SF-1449 The National Cancer Institute (NCI), Center for Cancer Research (CCR) and Urologic Oncology Branch (UOB) intends to procure the Brand Name Azure Biosystems c500 Gel/blot Imaging System. This is a combined synopsis/solicitation for commercial items, prepared in accordance with format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation: No. N02RC42643-61 includes all applicable provisions and clauses in effect through FAR FAC 2005-76 (August 2014) simplified procedures for commercial items. This solicitation is issued as a Request for Quotation in accordance with FAR 13.004. The North American Industry Classification System code is 334516 and the business size standard is 500 employees. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. I. Description of Requirement The Urologic Oncology Branch (UOB) propose the acquisition to support the mission of the NCI, UOB in performing basic and clinical research for the purposes of improving NCI's understanding of cancer, its diagnosis and treatment. UOB plans to acquire research equipment needed for rapid analysis of experimentally generated samples as well as samples from cancer patients participating in clinical trials at NCI and NCI-supported institutions. The equipment sought is an imaging system that improves the ability to detect and characterize critical differences between normal and tumor samples, as well as differences that expand NCI's understanding of cell and molecular biology. The new equipment will improve the productivity and results quality of ongoing work while reducing human labor and total program costs. The instrumentation requested will facilitate the molecular and genetic analysis of a variety of research and human tissue samples obtained in clinical trials in progress in the NCI's Urologic Oncology Branch (UOB) and at collaborating, NCI-supported institutions. NCI's efforts focus on oncogenic signaling pathways that contribute to the onset and progression of genitourinary (GU) malignancies, amongst which prostate, bladder and kidney cancers are the most prevalent and deadly. In the US alone, over 380,000 new GU cancer cases will be diagnosed in 2012, claiming more than 56,000 lives in the same period. The major goals of NCI's Urologic Oncology Branch (UOB) include the identification and characterization of new diagnostic and prognostic biomarkers of GU malignancies, as well as pharmacodynamic markers of treatment response. Acquisition of the proposed image analysis system will improve the accuracy and reproducibility or NCI's assays, while reducing human labor time and total program operating costs. The following Salient Characteristics must be provided: a. Supported Applications and System Space Requirements: The requested Azure Biosystems c500 imaging system will support the following applications: (1) blot detection applications (primarily immunodetection, but optionally other tagging modalities) in the infrared (IR) and near IR spectrum, (2) chemiluminescent detection, (3) IR and visible fluorescently labeled protein array detection, (4) DNA detection using ethidium bromide, and (5) visible light imaging. Because bench space in the NIH Clinical Research Center is so valuable, the footprint of the imaging system is an important consideration. The c500 system has compact dimensions (38cm X 55cm X 36cm), and is upgradeable to be capable of detecting other imaging dyes (e.g. CY5/Cy3/Cy2) in the visible light spectrum. b. Imaging Illumination Sources: The Azure Biosystems c500 imaging system has 2 sets of infrared, solid state laser diodes emitting at 660nm +/-3nm, and 785nm +/-3nm, respectively, for excitation and detection of IR dyes. Laser lifetime exceeds 40,000 hours of operation time before failure to minimize maintenance costs. Laser sources in this spectral range are far more powerful and efficient than LED sources, enabling lower limits of detection and lower system noise, thereby providing greater precision for discriminating small intensity differences, e.g., between spots on protein arrays. The c500 imaging system has a separate set of epi-illuminating blue LEDs (470nm) for excitation of DNA gels stained with safe (non-mutagenic) fluorescent dyes like "SYBR Safe" cyanine dye products. Trans-illumination systems in this spectral range have higher direct emission into the detector and unacceptably high noise to signal ratios. The c500 imaging system provides a slide-out, dual wavelength UV transilluminator light source at 302nm and 365nm for nucleic acid applications and various dyes, as well as a safety override enabling band excision from gels. When activated, the safety override switch will automatically turn off after 5 minutes to prevent damage to the system. The trans-illuminator is controlled by onboard image capture software and automatically turns off after each image capture to extend the life of the light source and minimize maintenance costs. The UV lamps for 302/365 nm illumination also have a lifetime exceeding 30,000 hrs. to reduce replacement costs. c. Imaging Detection System: The c500 imaging system uses an 8.3 megapixel scientific CCD sensor (3326 X 2504) with 16 bit analog to digital conversion. The CCD sensor uses regulated Peltier cooling (-50 ⁰C) to eliminate electronic noise in chemiluminescent imaging and has a motorized, 0.95 f-stop lens for light collection efficiency and short exposure times. Images are captured with a resolution range of 40um to 420um, enabling precision imaging of IR excited or chemiluminescent protein arrays. Fluorescent dyes are detected in the (710-730nm) to (815-830nm) ranges for the 700 and 800 channels, respectively, providing a high signal-to-noise ratio. IR dye detection is achieved at a minimum threshold of 700nm. The light sources for fluorescence detection provide field uniformity across the imaging area with CV values of +/- 5% as determined by measuring total intensity values across the entire illuminated imaging area. The system has a motorized 7 position filter wheel with 3 standard filters, and the choice of filter in the filter wheel is controlled by image capture software through the selection of an application or dye for ease-of-use. The 500c system has 420 DPI image output resolution to provide publication quality graphics. d. Field of View and Focusing: The maximum field of view of 20cm x 15cm enables high throughput imaging of multiple chemiluminescent and fluorescent blots and gels simultaneously. The system has automatic image focusing for ease-of-use without the need for users to define and set up protocols within the capture software. e. Image Capture, Analysis and Output Software: The 500c imaging system has 3 USB ports to accommodate information transfer to various devices like networks, printers and USB memory sticks. The system software (included at no additional cost) enables capture or display of single or multichannel images, while allowing viewing of up to 3 channel images superimposed or separated as individual channels. The capture software allows the user to see pixel intensity values by simply mousing over the image, providing immediate direct intensity comparisons. The capture software allows customizing imaging protocols and multiple binning modes (including 2X2, 3X3, 4X4, and 5X5) for enhanced chemiluminescence imaging and reduced exposure times. The c500 system comes standard with image analysis software with an unlimited number of installs, so users can have copies of the software on their desktop and laptop computers for further image processing or to separate image acquisition processing operations. II. Delivery: Contractor(s) shall deliver the item within 30 days after award to: NIH/NCI Bethesda, Maryland 20892. The Contractor that receives the award will be provided with the complete mailing address and point of contact. Upon delivery, contractor must notify the NCI Contracting Officer's Representative (COR) to schedule the installation date and time. III. Installation: To be carried out by the manufacturer and shall occur within approximately one week of delivery. IV. Payment: Payment shall be made after delivery, installation, and successful operation of equipment. Payment authorization requires submission and approval of invoice to the NCI COR and NIH Commercial Accounts. QUOTATIONS ARE DUE: September 15, 2014 at 4:00 pm EST. PROVISIONS AND CLAUSES: The following FAR provisions and clauses apply to this acquisition: FAR clause 52.212-1 INSTRUCTIONS TO OFFERORS-COMMERCIAL ITEMS (April 2014); FAR clause 52.212-3 OFFEROR REPRESENTATIONS AND CERTIFICATIONS-COMMERCIAL ITEMS- (May 2014) - WITH ADDENDA [REPRESENTATION BY CORPORATIONS REGARDING AN UNPAID DELINQUENT TAX LIABILITY OR A FELONY CONVICTION UNDER ANY FEDERAL LAW AND FAR 52.204-6, DATA UNIVERSAL NUMBERING SYSTEM (DUNS) NUMBER (July 2013)] CONTRACT TERMS AND CONDITIONS - COMMERCIAL ITEMS (May 2014) - WITH ADDENDA [STOP WORK ORDER, FAR 52.242-15 (August 1989) and YEAR 2000 WARRANTY - COMMERCIAL SUPPLY ITEMS (July 1997)]. FAR Clause 52.212-5: CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS-COMMERCIAL ITEMS (July 2014). The following FAR clauses cited in paragraph (b) of the clause at FAR 52.212-5 are also applicable to this acquisition; FAR Clause 52.203-6 Restrictions on Subcontractor Sales to the Government FAR Clause 52.219-4 Notice of Price Evaluation Preference for HUBZone Small Business Concerns. FAR Clause 52.219-6 Notice of Total Small Business Set-Aside FAR Clause 52.219-13, Notice of Set-Aside of Orders FAR Clause 52.219-28 Post Award Small Business Program Representation FAR Clause 52.222-3 Convict Labor FAR Clause 52.222-19 Child Labor-Cooperation with Authorities and Remedies FAR Clause 52.222-21 Prohibition of Segregated Facilities FAR Clause 52.222-26 Equal Opportunity FAR Clause 52.222-35 Equal Opportunity for Veterans FAR Clause 52.222-36 Equal Opportunity for Workers with Disabilities FAR Clause 52.222-37 Employment Reports on Veterans FAR Clause 52.223-18, Encouraging Contractor Policies to Ban Text Messaging While Driving FAR Clause 52.225-13 Restrictions on Certain Foreign Purchases FAR Clause 52.232-29, Terms for Financing of Purchases of Commercial Items Far Clause 52.232-33 Payment by Electronic Funds Transfer-System for Award Management FAR Clause 52.247-64 Preference for Privately Owned U.S.-Flag Commercial Vessels Full text copies of the representations and certifications for other cited provisions and clauses may be obtained on line at the NCI website at http://ncioa.cancer.gov/oa-internet/reference.jsp or from Kathy Elliott, Contract Specialist at Elliottk@mail.nih.gov. OFFERORS: Offers must be submitted on an SF-1449 with a completed 52.212-3 Offeror Representations and Certifications-Commercial Items-with DUNS Number Addendum, signed by an authorized representative of the offeror OR provide a copy of the valid certification registrations of the offeror's Central Contractor Registration (CCR) and Online Representations and Certifications Applications (ORCA) through sam.gov. Quotations must be received in the NCI-OA contracting office by 4:00 p.m. EST on September 15, 2014. Written questions are due by 12:00 pm EST on September 12, 2014. Please refer to solicitation number N02RC42643-61 on all correspondence. No collect calls will be accepted. Faxed or electronic mail quotations will be accepted. All questions shall be in writing and may be addressed to the aforementioned individual noted above. No collect calls will be accepted. In order to receive an award, contractors must be registered and have valid certification in the Central Contractor Registration (CCR) and the Online Representations and Certifications Applications (ORCA) through sam.gov.
 

Web Link

FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/RCB/N02RC42643-61/listing.html)
 

Place of Performance

Address: NIH NCI, Bethesda, Maryland, 20892, United States

Zip Code: 20892
 

Record

SN03504374-W 20140912/140910235212-7191882c455cb89d6a075a67299b5abb (fbodaily.com)
 

Source

FedBizOpps Link to This Notice
(may not be valid after Archive Date)

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