SOLICITATION NOTICE
88 -- Custom Bred Mice
- Notice Date
- 11/6/2014
- Notice Type
- Presolicitation
- NAICS
- 112990
— All Other Animal Production
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions, 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, Maryland, 20892-7902
- ZIP Code
- 20892-7902
- Solicitation Number
- HHS-NIH-NHLBI-CSB-HI-2015-027-CDB
- Archive Date
- 11/28/2014
- Point of Contact
- Chris Bocus, Phone: 3014027888
- E-Mail Address
-
chris.bocus@nih.gov
(chris.bocus@nih.gov)
- Small Business Set-Aside
- N/A
- Description
- INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI), Office of Acquisitions (OA), on intends to negotiate and award a purchase order on a sole source noncompetitive basis to The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, to provide custom bred mice needed to test the use of multi-photon microscopy in tissue observation for the Systems Biology Center (SBC) of the Division of Intramural Research (DIR). PROCUREMENT: The contractor shall rederive two strains, Flk1GFP and Flt1-tdsRed, into their maximum barrier breeding facility using C57BL/6J oocyte donors. Breeding colonies will be established as Heterozygous x Wildtype or reciprocal matings. Once the colonies are established, there will be weekly shipments of 3 Het Flt1-tdsRed mice and 2 Het Flk1GFP mice at 8 weeks of age over the period of 97 weeks. Prior to shipment, the contractor will genotype the mice by performing tail snips and running PCR tests prior to ensure the proper strain is shipped. The first shipment of 3 Het Flt1-tdsRed mice and 2 Het Flk1GFP mice should occur approximately 9 months after the delivery of donor males to contractor's facilities. The colonies will be removed one month after the final shipment. BACKGROUND: The mission of the NHLBI Division of Intramural Research (DIR) is to perform robust scientific and clinical research leading to a better understanding of biology and clinical pathology. To attain this goal, we have built a strong basic science foundation and coupled it closely with innovative technology development and outstanding clinical research both at the NIH Clinical Center and in partnership with local hospitals. The purview of our research is broad, encompassing investigations into the basic principles of molecular, cellular, and organ-level biology and their relationship to disease. Some current areas of fundamental interest include single molecule structure; protein assembly; molecular and cell biology; cell signaling and motility; membrane trafficking; physiology; systems biology; engineering and technology development. Insights into disease mechanisms derived from basic studies form the basis for translational research into new diagnostic and therapeutic approaches. DIR investigators also conduct concept-based clinical studies in the areas of interventional and surgical cardiology; pulmonary medicine; sickle cell anemia; bone marrow transplant; and hematologic disorders. The Systems Biology Center (SBC) of the Division of Intramural Research of the National Heart Lung and Blood Institute is involved in the study of the structure/function relationships of different mouse tissues. Detailed examine of tissue morphology (i.e., the basic layout of a tissue's structural components), coupled with monitoring of cellular metabolism, will allow the lab to understand how different cell types respond to perturbation. With this information, the lab can apply its findings to disease states, where changes in overall structure might drastically impact a tissue's physiological function. SBC plans to investigate the morphological and functional characteristics of mouse skeletal muscle, kidney, and brain. Concurrent with these physiological measurements will be the continued development of the multi-photon microscopy motion tracking technique, which enables scientific staff to follow real-time metabolic changes in the living mouse. Multi-photon microscopy is a relatively new tool that uses pulsed infrared light to image metabolism deep within intact tissues in living animals to levels of resolution that no other technique can approach. In particular, this study will examine fluorescent characteristics in tissues of live, anesthetized mice and on tissues harvested from mice to determine the optical and experimental parameters necessary to use this technique on live animals in more complicated experiments in the future. Continued development of this technique is important for future studies of many different biological processes applicable to human disease. Fluorescently-labeled protein can give us structural information about the small functional components of cells within tissue, in vivo. In this study, the SBC will specifically emphasize procedures for imaging many aspects of muscle, kidney, and brain. Though SBC's staff has gained experience in imaging skeletal muscle and has improved their motion tracking software immensely, they have yet to examine physiological perturbations to the muscle (i.e., exercise stimulation) and have just begun studies on the kidney and brain. Therefore, the overall goal of this protocol is broad-spectrum in vivo imaging of mouse tissues, with a specific emphasis on tracking metabolic changes in response to insult. The breeding project is for a mouse that expresses channelrhodopsin in the cell membranes of heart and skeletal muscle. Channelrhodopsin is a light gated channel that opens when exposed to blue light. Opening this channel in the membrane of heart and skeletal muscle causes muscle contraction to occur. Under the microscope, the user can control when and where the muscle is exposed to blue light thus allowing the user to spatially and temporally control muscle contraction in groups of fibers or even single fibers. This technique will allow SBC's staff to study mitochondrial energy metabolism without the difficulties associated with motion and swelling caused by whole muscle stimulation. Additionally, lab staff will be able to evaluate the blood flow response to contraction of selected regions of muscle. PURPOSE AND OBJECTIVE: This acquisition is for the purpose of procuring custom bred mice needed to test the use of multi-photon microscopy in tissue observation for the Systems Biology Center (SBC) of the Division of Intramural Research (DIR). ANTICIPATED PERIOD OF PERFORMANCE: To be negotiated upon award. JUSTIFICATION: The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted as prescribed in FAR Part 10-Market Research. The Jackson Laboratory is the only source capable of fulfilling the government's requirement. The SBC has used mice rederived by The Jackson Laboratory for its past experiments and must continue to obtain mice from The Jackson Laboratory in order to maintain consistency in research. The use of other sources would invariably cause variation in this ongoing research and even the slightest variation in the breeding process could skew the lab's data. The success of this research is contingent upon maintaining consistency in the breeding process. REGULATORY AUTHORITY: This acquisition is conducted under the authority of 41 U.S.C. 253(c) as set forth in FAR Part 13.106-1 (b) (1), Soliciting from a single source not exceeding the simplified acquisition threshold, and is exempt from the requirements of FAR Part 6, Competition Requirements. ADDITIONAL INFORMATION: Industry Classification (NAICS) Code is 112990, All Other Animal Production and the Small Business Size Standard is $0.75 Million. This acquisition is conducted under the procedures as prescribed in FAR subpart 12-Acquisition of Commercial Items and FAR subpart 13-Simplified Acquisition Procedures. The solicitation document, the incorporated provisions and clauses are those in the Federal Acquisition Circular 2005-77, effective October 14, 2014. The determination by the Government to award a purchase order on a sole source noncompetitive basis to provide custom bred mice needed to test the use of multi-photon microscopy in tissue observation for the Systems Biology Center (SBC) of the Division of Intramural Research (DIR) to The Jackson Laboratory, 600 Main Street, Bar Harbor, ME, 04609-1500, is based upon the market research conducted in accordance with one or more of the techniques specified in FAR Part 10-Market Research, Subpart 10.002(b)(2). This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses must be received by November 13, 2014 at 12:00 p.m. EST. Responses must reference synopsis number HHS-NIH-NHLBI-CSB-HI-2015-027-CDB, may be submitted to the National, Heart, Lung and Blood Institute, Office of Acquisition, COAC Services Branch, 6701 Rockledge Drive, Suite 6121A, Bethesda, Maryland 20892-7902, Attention: Chris Bocus. Response may be submitted electronically to chris.bocus@ nih.gov. Faxes will not be accepted. Responses will only be accepted if dated and signed by an authorized company representative. "All responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency."
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NHLBI/HHS-NIH-NHLBI-CSB-HI-2015-027-CDB/listing.html)
- Place of Performance
- Address: Bethesda, Maryland, 20892, United States
- Zip Code: 20892
- Zip Code: 20892
- Record
- SN03568494-W 20141108/141106234826-4fee43e647ca77ee548cc4fab5f622ac (fbodaily.com)
- Source
-
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