SOLICITATION NOTICE
70 -- Cloning and purification of IDH2 mutants software
- Notice Date
- 5/29/2015
- Notice Type
- Presolicitation
- NAICS
- 541511
— Custom Computer Programming Services
- Contracting Office
- Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support/Simplified Acquisitions, 31 Center Drive, Room 1B59, Bethesda, Maryland, 20892
- ZIP Code
- 20892
- Solicitation Number
- HHS-NIH-NIDA-SSSA-NOI-15-225
- Archive Date
- 6/24/2015
- Point of Contact
- Andrea McGee, Phone: 3014358718
- E-Mail Address
-
amcgee@nida.nih.gov
(amcgee@nida.nih.gov)
- Small Business Set-Aside
- Total Small Business
- Description
- SOLICITATION NUMBER: HHS-NIH-NIDA-SSSA-NOI-15-225 TITLE: Cloning and purification of IDH2 mutants software INTRODUCTION This is a pre-solicitation non-competitive (notice of intent) synopsis to award a contract without providing for full or open competition (including brand-name). THIS IS A NOTICE OF INTENT, NOT A REQUEST FOR PROPOSAL. A SOLICITATION WILL NOT BE ISSUED AND PROPOSALS WILL NOT BE REQUESTED. The National Institute on Drug Abuse (NIDA), Consolidated Station Support and Simplified Acquisitions (CSS/SA) Branch intends to negotiate and award a purchase order to BERYLLIUM CORP for small molecules without providing for full and open competition (including brand-name). NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE This acquisition is unrestricted. The associated NAICS code 325414 with a size standard of 500 employees. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through the through Federal Acquisition Circular (FAC) 2005-80 effective April 10, 2015. This acquisition is for a commercial item or service and is conducted under the procedures as prescribed in FAR subpart 13-Simplified Acquisition Procedures. This acquisition is NOT expected to exceed the simplified acquisition threshold. STATUTORY AUTHORITY This acquisition is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13-Simplified Acquisition Procedures, Subpart 13.106-1. DESCRIPTION OF REQUIREMENT Purpose and Objectives: The purpose of this requisition is to acquire cloning and purification of IDH2 mutants. Project Description: NCATS is a translational science center that prides itself on its tremendously productive pipeline and is innovative in a number of ways. First, our quantitative high throughput screening (qHTS) paradigm, which tests all compounds at multiple concentrations in the primary screen, is unique. However, more and more academic and pharmaceutical groups are now adopting it in their own operations due to the enhanced data generation that enable compound bioactivity assessment and probe production efficiency. This efficiency derives not only from savings in post-screen cherry-picking, reconfirmation, dose-response testing, and initial SAR assessment, but also from the ability to perform reliable "virtual counter screens," thus allowing us to make more informed decisions on which scaffolds to pursue for probe development. Second, the NCATS is distinctive in its focus on collaboration with outside investigators to produce high-quality chemical probes to define new biology. This focus is reflected in our unprecedented Outreach program, which reaches hundreds of potential new investigators every year, and accounts for much of the new science being done at the NCATS. Third, the NCATS is uniquely focused on new technologies and paradigms to improve the process of probe development, typified by qHTS, our informatics platform, our utilization of structural biology to accelerate probe development, development of new synthetic and analytical chemistry paradigms. Discoveries about the molecular basis of disease provide unprecedented opportunities to translate research findings into new medicines. However, developing a brand-new drug takes an enormous amount of time, money and effort, mainly because of bottlenecks in the therapeutic development process. Delays and barriers can mean that translation of a promising molecule into an approved drug often takes more than 14 years. It is crucial to advance strategies to reduce this time frame, decrease costs and improve success rates. As such, NCATS is in need milligram quantities of IDH2 mutantsR172K and R140Q. This acquisition requires cloning and scale up and purification of the two mutants, the detailed steps will be described in contractor requirements. This project has been a long term NCATS project, which was initiated through the Molecular Libraries and has been accepted into the NCI CBC and hence is tied to strict deadlines. Specifically, NCATS requires the following: The services needed, requires the vendor to perform the following tasks: Cloning and scale-up: •The vendor shall create IDH2(40-452-His6/R172K) and IDH2(40-452-His6/R140Q) using Site Directed Mutagenesis on VCID 10487 (wild-type IDH2; 40-452). •The vendor shall transform these vectors into a baculovirus expression system. •The vendor shall prepare a 10L culture of both IDH2 R140Q and IDH2 R172K for purification. Purification: •The vendor shall purify both IDH2-R172K and IDH2-R140Q from a 10L culture following the protocol that had been developed for the wild-type protein. •The vendor shall deliver the yield of purified protein up to 10 milligrams (minimum of 3 mg) from each protein prep to NCATS in the buffer and concentration of their choosing. •The vendor shall deliver each protein with accompanying QC results including SDS-PAGE and Experion purity analysis. GOVERNMENT RESPONSIBILITIES NCATS will specify the buffer in which proteins shall be delivered in upon the completion of cloning and scale up. DELIVERY OR DELIVERABLES Vendor is responsible for delivering between 3 mg and 10 mg of IDH2-R172K and IDH2-R140Q 20 weeks upon award is made. Each mutant delivered shall provide QC results, including SDS-PAGE and Experion purity analysis. CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME) DETERMINATION The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted. Specifically, BERYLLIUM CORP is the only vendor in the current market place that can provide the unique services required by the of National Center for Advancing Translational Sciences. In accordance with FAR part 10, extensive market research was conducted to reach this determination. Specifically, a small business sources sought notice was posted to FedBizOpps referencing the above detailed requirements and no responses were received. Therefore, only BERYLLIUM CORP is capable of meeting the needs of this requirement. The intended source is: Beryllium Corp 3 Preston Ct Bedford, MA 01730 CLOSING STATEMENT THIS SYNOPSIS IS NOT A REQUEST FOR COMPETITIVE PROPOSALS. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: unit price, list price, shipping and handling costs, the delivery period after contract award, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov." A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses shall be submitted no later than June 9, 2015 at 4:30pm EST. Responses may be submitted electronically to amcgee@nih.gov or by U.S. mail to the National Institute of Drug Abuse (NIDA), Station Support / Simplified Acquisition Branch (SS/SA), Attention: Andrea McGee. Fax responses will not be accepted.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/spg/HHS/NIH/NIDA-2/HHS-NIH-NIDA-SSSA-NOI-15-225/listing.html)
- Place of Performance
- Address: 9800 Medical Center Driver, Rockville, Maryland, 20850, United States
- Zip Code: 20850
- Zip Code: 20850
- Record
- SN03747239-W 20150531/150529235314-7fd0a7b9b3a43a9a073d7658237edf63 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
(may not be valid after Archive Date)
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