SPECIAL NOTICE
99 -- Bacterial Artificial Chromosome Library
- Notice Date
- 6/16/2015
- Notice Type
- Special Notice
- NAICS
- 334516
— Analytical Laboratory Instrument Manufacturing
- Contracting Office
- Agricultural Research Service - Pacific West Area
- ZIP Code
- 00000
- Solicitation Number
- 593-5320-016-0005
- Archive Date
- 7/7/2015
- Point of Contact
- Carlee Ulibas, Phone: 808-932-2120
- E-Mail Address
-
carlee.ulibas@ars.usda.gov
(carlee.ulibas@ars.usda.gov)
- Small Business Set-Aside
- N/A
- Description
- THIS IS NOT A REQUEST FOR QUOTE: This is a special notice for a commercial item. The North American Industry Classification System (NAICS) Code for this acquisition is 334516 and the small business size standard is 500 employees or less. The USDA, Agriculture Research Service (ARS), Daniel K. Inouye, Pacific Basin Agricultural Research Center in Hilo, Hawaii intends to sole source a Bacterial Artificial Chromosome (BAC) Library to Intact Genomics, Inc. This company has been found to use a unique method to create bacterial artificial chromosomes (BAC) Libraries by preparing the target organism DNA by searing rather than by enzyme digestion. Since enzyme restriction sites are not distributed evenly across any given genome, the resulting libraries do not contain representation of the entire genome- there are missing pieces with the enzyme restriction method used by other companies. With the random shear method utilized by this company, the coverage of the genome is unbiased and more likely statistically to contain the entire genome withing the number of clones (184,320 with estimated 100 kb insert) provided by this company. The company also uses a vector for cloning that has a unique property that selects against empty vectors, ensuring a better chance of achieving each of the 184,320 clones to contain inserts. We need a genomic library that is comprehensive in order to identify and mine genomic sequences for our research. The USDA, Agriculture Research Service will be acquiring the following: 001: Creation of an unbiased (Bacterial Artificial Chromosome or BAC) genomic library of Anthurium species consisting of 184,320 clones, a 3.6 x genome coverage based on an average insert size of 100 kb. The above service includes making High Molecular Weight DNA from customer supplied plant tissue, use of proprietary methods to generate randomly sheared DNA, ligate fragments into a BAC cloning vector and transformed into E. Coli Strain DH10B cells. 002: Plate out the BAC library and array positive colones in 384 well format plates. The above service includes the tittering of each positive BAC clone into bar coded 384 well plates and stored at 80 degrees C. 003: Shipping of library on dry ice. All interested parties are encouraged to provide information on their capabilities to meet this requirement. In order to be considered a potential source, interested parties must submit clear and convincing documentation demonstrating the capabilities to satisfy the requirement specified above and advantages of the Government to consider another source. The documentation shall include prices and descriptive literature containing sufficient technical documentation to establish a bona fide capability to meet this requirement. Only written responses will be considered. All responses from responsible sources will be fully considered. As a result of analyzing responses to this synopsis, the contracting officer may determine that a solicitation will be issued. If a solicitation is issued, no additional synopsis will be published. Any such solicitation will be issued to the intended schedule vendor and all firms that respond to this synopsis. If no affirmative written response is received by Thursday, June 22, 2015, an award will be pursued with the company mentioned above. Delivery is to the USDA, Agriculture Research Service, DKI-PBARC 64 Nowelo St Hilo, HI 96720 The provision at 52.212-3, Offeror Representations and Certifications-Commercial Items, applies to this acquisition. The clause at 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. The clause at 52.212-5, Contract Terms and Conditions Required To Implement Statutes or Executive Orders-Commercial Items applies to this acquisition, with these additional FAR clauses cited in the clause applicable to the acquisition: 52.204-7 System for Award Management; 52.209-6, Protecting the Government's Interest When Subcontracting with Contractors Debarred, Suspended, or Proposed for Debarment; 52.209-10, Prohibition on Contracting with Inverted Domestic Corporations; 52.222-21, Prohibition of Segregated Facilities; 52.222-26, Equal Opportunity; 52.222-35, Equal Opportunity for Veterans; 52.222-36, Affirmative Action for Workers with Disabilities; 52.222-37, Employment Reports on Veterans; 52.223-18, Contractor Policies to Ban Text Messaging While Driving; 52.225-3, Buy American Act-Free Trade Agreements-Israeli Trade Act, Alt II; 52.225-13, Restrictions on Certain Foreign Purchases; 52.232-33, Payment by Electronic Funds Transfer-System for Award Management; 52.232-36, Payment by Third Party. The full text of all clauses and provisions are available at www.acqnet.gov and http://www.dm.usda.gov/procurement/policy/agar_x/agarbase/subchaph.html#22871 All businesses wishing to conduct business with the US. Federal Government are required to obtain a DUNS number and register in SAMS (System for Award Management). The System for Award Management (SAM) is a Web-enabled government wide application that collects, validates, stores and disseminates business information about the federal government's trading partners in support of the contract award, grants and the electronic payment processes. All businesses must be registered and have an active registration, when submitting their quote. Please note your DUNS# on your quote and your payment preference.
- Web Link
-
FBO.gov Permalink
(https://www.fbo.gov/notices/8343b36d10db59973d3502ed74c83411)
- Place of Performance
- Address: USDA-ARS-DKI PBARC, 64 Nowelo St, Hilo, Hawaii, 96720, United States
- Zip Code: 96720
- Zip Code: 96720
- Record
- SN03765415-W 20150618/150616234513-8343b36d10db59973d3502ed74c83411 (fbodaily.com)
- Source
-
FedBizOpps Link to This Notice
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